دورية أكاديمية
أعرض في EDS

Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection.

التفاصيل البيبلوغرافية
العنوان: Rapid bioprinting of conjunctival stem cell micro-constructs for subconjunctival ocular injection.
المؤلفون: Zhong Z; Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA., Deng X; Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA., Wang P; Materials Science and Engineering Program, University of California San Diego, La Jolla, CA, 92093, USA., Yu C; Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA., Kiratitanaporn W; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA., Wu X; Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA., Schimelman J; Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA., Tang M; Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA., Balayan A; School of Medicine, University of California San Diego, La Jolla, CA, 92093, USA., Yao E; Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA., Tian J; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA., Chen L; Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA., Zhang K; Department of Ophthalmology, University of California San Diego, La Jolla, CA, 92093, USA. Electronic address: kangzhang@gmail.com., Chen S; Department of NanoEngineering, University of California San Diego, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California San Diego, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA. Electronic address: chen168@eng.ucsd.edu.
المصدر: Biomaterials [Biomaterials] 2021 Jan; Vol. 267, pp. 120462. Date of Electronic Publication: 2020 Oct 23.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8100316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5905 (Electronic) Linking ISSN: 01429612 NLM ISO Abbreviation: Biomaterials Subsets: MEDLINE
أسماء مطبوعة: Publication: <1995-> : Amsterdam : Elsevier Science
Original Publication: [Guilford, England] : IPC Science and Technology Press, 1980-
مواضيع طبية MeSH: Bioprinting*, Animals ; Cell Differentiation ; Hydrogels ; Printing, Three-Dimensional ; Quality of Life ; Rabbits ; Stem Cells ; Tissue Engineering
مستخلص: Ocular surface diseases including conjunctival disorders are multifactorial progressive conditions that can severely affect vision and quality of life. In recent years, stem cell therapies based on conjunctival stem cells (CjSCs) have become a potential solution for treating ocular surface diseases. However, neither an efficient culture of CjSCs nor the development of a minimally invasive ocular surface CjSC transplantation therapy has been reported. Here, we developed a robust in vitro expansion method for primary rabbit-derived CjSCs and applied digital light processing (DLP)-based bioprinting to produce CjSC-loaded hydrogel micro-constructs for injectable delivery. Expansion medium containing small molecule cocktail generated fast dividing and highly homogenous CjSCs for more than 10 passages in feeder-free culture. Bioprinted hydrogel micro-constructs with tunable mechanical properties enabled the 3D culture of CjSCs while supporting viability, stem cell phenotype, and differentiation potency into conjunctival goblet cells. These hydrogel micro-constructs were well-suited for scalable dynamic suspension culture of CjSCs and were successfully delivered to the bulbar conjunctival epithelium via minimally invasive subconjunctival injection. This work integrates novel cell culture strategies with bioprinting to develop a clinically relevant injectable-delivery approach for CjSCs towards the stem cell therapies for the treatment of ocular surface diseases.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
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معلومات مُعتمدة: P30 NS047101 United States NS NINDS NIH HHS; R01 EB002185 United States EB NIBIB NIH HHS; P30 CA023100 United States CA NCI NIH HHS; R01 EB021857 United States EB NIBIB NIH HHS; R21 EY031122 United States EY NEI NIH HHS
فهرسة مساهمة: Keywords: 3D culture; Bioprinting; Conjunctival stem cell; Injectable delivery; Ocular surface regeneration; Stem cell therapy
المشرفين على المادة: 0 (Hydrogels)
تواريخ الأحداث: Date Created: 20201031 Date Completed: 20210524 Latest Revision: 20240330
رمز التحديث: 20240330
مُعرف محوري في PubMed: PMC7719077
DOI: 10.1016/j.biomaterials.2020.120462
PMID: 33129190
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-5905
DOI:10.1016/j.biomaterials.2020.120462