دورية أكاديمية
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Characterization of a Plasmodium falciparum PHISTc protein, PF3D7_0801000, in blood- stage malaria parasites.

التفاصيل البيبلوغرافية
العنوان: Characterization of a Plasmodium falciparum PHISTc protein, PF3D7_0801000, in blood- stage malaria parasites.
المؤلفون: Nagaoka H; Division of Malaria Research, Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan., Kanoi BN; Division of Malaria Research, Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan., Morita M; Division of Malaria Research, Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan., Nakata T; Division of Malaria Research, Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan., Palacpac NMQ; Department of Malaria Vaccine Development, Research Institute for Microbial Diseases, Osaka University, Suita 565-0871, Japan., Egwang TG; Med Biotech Laboratories, P.O. Box 9364, Kampala, Uganda., Horii T; Department of Malaria Vaccine Development, Research Institute for Microbial Diseases, Osaka University, Suita 565-0871, Japan., Tsuboi T; Division of Malaria Research, Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan., Takashima E; Division of Malaria Research, Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. Electronic address: takashima.eizo.mz@ehime-u.ac.jp.
المصدر: Parasitology international [Parasitol Int] 2021 Feb; Vol. 80, pp. 102240. Date of Electronic Publication: 2020 Nov 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9708549 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-0329 (Electronic) Linking ISSN: 13835769 NLM ISO Abbreviation: Parasitol Int Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam ; New York : Elsevier, c1997-
مواضيع طبية MeSH: Antibodies, Protozoan/*analysis , Merozoites/*chemistry , Plasmodium falciparum/*chemistry , Protozoan Proteins/*analysis, Malaria Vaccines/chemical synthesis ; Malaria, Falciparum/prevention & control
مستخلص: During intraerythrocytic development Plasmodium falciparum deploys numerous proteins to support erythrocyte invasion, intracellular growth and development, as well as host immune evasion. Since these proteins are key for parasite intraerythrocytic survival and propagation, they represent attractive targets for antimalarial vaccines. In this study we sought to characterize a member of the PHISTc family of proteins, PF3D7_0801000, as a potential vaccine target. Using the wheat germ cell-free system we expressed the N-terminal region of PF3D7_0801000 (G 93 -L 494 , PF3D7_0801000N) and generated specific immune sera. We observed that PF3D7_0801000 localizes in merozoites, and antibodies against PF3D7_0801000N modestly inhibit P. falciparum parasite growth in in vitro culture. Sliding window analysis of the coding sequence revealed that pf3d7_0801000n is relatively conserved among African parasite isolates. Antibody profiles in a malaria-exposed Ugandan population revealed that PF3D7_0801000N is strongly immunoreactive with antibody acquisition increasing with age. Taken together, these findings suggest the need for further evaluation of PF3D7_0801000 for its role in merozoite invasion and utility as an asexual blood-stage vaccine candidate antigen.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Blood-stage vaccine; Malaria; PF3D7_0801000; PHISTc; Plasmodium falciparum
المشرفين على المادة: 0 (Antibodies, Protozoan)
0 (Malaria Vaccines)
0 (Protozoan Proteins)
تواريخ الأحداث: Date Created: 20201104 Date Completed: 20210623 Latest Revision: 20210623
رمز التحديث: 20221213
DOI: 10.1016/j.parint.2020.102240
PMID: 33147497
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-0329
DOI:10.1016/j.parint.2020.102240