دورية أكاديمية
The role of IL-10 in immune responses against Pseudomonas aeruginosa during acute lung infection.
العنوان: | The role of IL-10 in immune responses against Pseudomonas aeruginosa during acute lung infection. |
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المؤلفون: | Belo VA; Departmento de Microbiologia E Imunologia, Universidade Federal de Alfenas, Alfenas, Brazil., Pereira JA; Departmento de Microbiologia E Imunologia, Universidade Federal de Alfenas, Alfenas, Brazil., Souza SFD; Departmento de Microbiologia E Imunologia, Universidade Federal de Alfenas, Alfenas, Brazil., Tana FL; Departmento de Microbiologia E Imunologia, Universidade Federal de Alfenas, Alfenas, Brazil., Pereira BP; Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Brazil., Lopes DO; Laboratório de Biologia Molecular, Universidade Federal de São João Del-Rei (CCO), Divinópolis, Brazil., Ceron CS; Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Alfenas, Brazil., Novaes RD; Departamento de Biologia Estrutural, Universidade Federal de Alfenas, Alfenas, Brazil., Corsetti PP; Laboratório de Imunologia das Doenças Infecciosas E Crônicas, Universidade José Do Rosário Vellano, Alfenas, Minas Gerais, Brazil., de Almeida LA; Departmento de Microbiologia E Imunologia, Universidade Federal de Alfenas, Alfenas, Brazil. leonardo.almeida@unifal-mg.edu.br. |
المصدر: | Cell and tissue research [Cell Tissue Res] 2021 Mar; Vol. 383 (3), pp. 1123-1133. Date of Electronic Publication: 2020 Nov 09. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417625 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0878 (Electronic) Linking ISSN: 0302766X NLM ISO Abbreviation: Cell Tissue Res Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin, New York, Springer-Verlag. |
مواضيع طبية MeSH: | Lung*/immunology , Lung*/pathology, Interleukin-10/*immunology , Pseudomonas Infections/*immunology , Pseudomonas aeruginosa/*immunology, Animals ; Immunity ; Matrix Metalloproteinase 9/immunology ; Mice ; Mice, Inbred C57BL ; Mice, Knockout |
مستخلص: | Pseudomonas aeruginosa is considered an opportunistic pathogen of great clinical importance. The clearance of this bacterium occurs through recognition of the pathogen by innate immune system receptors, leading to a lung inflammatory response. However, this response must be controlled via immunoregulatory pathways. In this study, we evaluate the role of endogenous murine IL-10 after acute infection with the virulent strain P. aeruginosa PA14. To assess the role of IL-10, intratracheal infection with the PA14 strain was performed in C57BL/6 or IL-10 KO mice. The PA14 strain was recovered in both types of animals, although IL-10 KO mice presented a higher number of viable bacteria in the lung when compared to the C57BL/6 group. Histopathological and stereological analyses showed that IL-10 KO mice had higher tissue damage and inflammatory infiltrate when compared to control animals. The activity of MMP-9 but not MMP-2, as well as IL-6 and TNF-α expression, were augmented in the lungs of infected animals and was much more evident in IL-10 KO animals when compared to the other analyzed groups. This work indicates that endogenous IL-10 control P. aeruginosa infection, the expression of pro-inflammatory genes, MMP-9 activity and histopathological processes of the infectious process in question. |
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فهرسة مساهمة: | Keywords: Immunoregulation; Inflammatory response; Interleukin-10; Lung infection; MMP-9; P. aeruginosa |
المشرفين على المادة: | 0 (IL10 protein, mouse) 130068-27-8 (Interleukin-10) EC 3.4.24.35 (Matrix Metalloproteinase 9) EC 3.4.24.35 (Mmp9 protein, mouse) |
تواريخ الأحداث: | Date Created: 20201109 Date Completed: 20211001 Latest Revision: 20211001 |
رمز التحديث: | 20221213 |
DOI: | 10.1007/s00441-020-03308-4 |
PMID: | 33165659 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1432-0878 |
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DOI: | 10.1007/s00441-020-03308-4 |