دورية أكاديمية
Cytotoxicity of botanicals and isolated phytochemicals from Araliopsis soyauxii Engl. (Rutaceae) towards a panel of human cancer cells.
| العنوان: | Cytotoxicity of botanicals and isolated phytochemicals from Araliopsis soyauxii Engl. (Rutaceae) towards a panel of human cancer cells. |
|---|---|
| المؤلفون: | Mbaveng AT; Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: armbatsa@yahoo.fr., Noulala CGT; Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon. Electronic address: cedric.noulala@hotmail.fr., Samba ARM; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon; Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon. Electronic address: annecesarine@gmail.com., Tankeo SB; Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany; Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon. Electronic address: simplicetankeo@yahoo.fr., Fotso GW; Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon. Electronic address: ghis152001@gmail.com., Happi EN; Department of Chemistry, Faculty of Science, University of Douala, Douala, Cameroon. Electronic address: ngeufa@yahoo.fr., Ngadjui BT; Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon. Electronic address: ngadjuibt@yahoo.fr., Beng VP; Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. Electronic address: v.penlap@yahoo.fr., Kuete V; Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, University of Mainz, Staudinger Weg 5, 55128, Mainz, Germany; Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon. Electronic address: kuetevictor@yahoo.fr., Efferth T; Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon. Electronic address: efferth@uni-mainz.de. |
| المصدر: | Journal of ethnopharmacology [J Ethnopharmacol] 2021 Mar 01; Vol. 267, pp. 113535. Date of Electronic Publication: 2020 Nov 06. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Sequoia Original Publication: Lausanne, Elsevier Sequoia. |
| مواضيع طبية MeSH: | Rutaceae*/chemistry, Antineoplastic Agents, Phytogenic/*pharmacology , Apoptosis/*drug effects , Neoplasms/*drug therapy , Phytochemicals/*pharmacology , Plant Extracts/*pharmacology, Antineoplastic Agents, Phytogenic/isolation & purification ; Apoptosis Regulatory Proteins/metabolism ; Benzoxepins/pharmacology ; Cell Cycle Checkpoints/drug effects ; Dose-Response Relationship, Drug ; HCT116 Cells ; Hep G2 Cells ; Humans ; Inhibitory Concentration 50 ; Limonins/pharmacology ; Membrane Potential, Mitochondrial/drug effects ; Mitochondria/drug effects ; Mitochondria/metabolism ; Mitochondria/pathology ; Neoplasms/pathology ; Oxidative Stress/drug effects ; Phytochemicals/isolation & purification ; Plant Extracts/isolation & purification ; Reactive Oxygen Species/metabolism ; Signal Transduction |
| مستخلص: | Ethnopharmacological Relevance: Araliopsis soyauxii Engl. (Rutaceae) is a Cameroonian medicinal plant traditionally used to treat lung diseases, malaria, and gonorrhea. It has been demonstrated that infectious disease contribute to about 20% of all human tumours. Aims of the Study: (1) To perform a phytochemical investigation of the dichloromethane-methanol 1:1 extracts of the bark (ASB), roots (ASR), and leaves (ASL) from Araliopsis soyauxii; (2) to evaluate the cytotoxicity of extracts and isolated compounds; (3) to determine the mode of induction of apoptosis of ASB and kihadanin B (12). Materials and Methods: Fourteen constituents of the crude extracts were isolated by column chromatography, while spectroscopic techniques were used for structural elucidation. The resazurin reduction assay (RRA) was applied to determine the cytotoxicity of samples towards a panel of 9 cancer cell lines. For caspases activity, the Caspase-Glo assay was used; flow cytometry was applied to investigate the cell cycle distribution (PI staining), apoptosis (annexin V/PI staining), mitochondrial membrane potential (MMP; JC-1 staining), and the reactive oxygen species (ROS; H Results: Phytochemical investigations of botanicals (ASB, ASR, and ASL) led to the isolation of 14 compounds. Extract ASB, obacunone (11), kihadanin B (12) as well as doxorubicin (control drug) revealed cytotoxicity towards the 9 cancer cell lines tested. The IC Conclusion: This study demonstrated that Araliopsis soyauxii is a potential source of cytotoxic phytochemicals such as kihadanin B and that ASB and compound 12. Extract and compounds will be explored further to develop anticancer drugs. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Araliopsis soyauxii; Cytotoxicity; Kihadanin B; Multidrug resistance; Rutaceae |
| المشرفين على المادة: | 0 (Antineoplastic Agents, Phytogenic) 0 (Apoptosis Regulatory Proteins) 0 (Benzoxepins) 0 (Limonins) 0 (Phytochemicals) 0 (Plant Extracts) 0 (Reactive Oxygen Species) 751-03-1 (obacunone) |
| تواريخ الأحداث: | Date Created: 20201109 Date Completed: 20210302 Latest Revision: 20210302 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jep.2020.113535 |
| PMID: | 33166626 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7573 |
|---|---|
| DOI: | 10.1016/j.jep.2020.113535 |