On the Mechanism of Self-Assembly by a Hydrogel-Forming Peptide.

التفاصيل البيبلوغرافية
العنوان:	On the Mechanism of Self-Assembly by a Hydrogel-Forming Peptide.
المؤلفون:	Braun GA; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.; Department of Biochemistry and Structural Biology, Centre for Molecular Protein Science, Lund University, Lund SE-22100, Sweden., Ary BE; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States., Dear AJ; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.; Paulson School of Engineering and Applied Science, Harvard University, Cambridge, Massachusetts 02138, United States., Rohn MCH; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States., Payson AM; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States., Lee DSM; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States., Parry RC; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States., Friedman C; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States., Knowles TPJ; Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, U.K.; Cavendish Laboratory, University of Cambridge, J J Thomson Avenue, Cambridge CB3 0HE, U.K., Linse S; Department of Biochemistry and Structural Biology, Centre for Molecular Protein Science, Lund University, Lund SE-22100, Sweden., Åkerfeldt KS; Department of Chemistry, Haverford College, Haverford, Pennsylvania 19041, United States.
المصدر:	Biomacromolecules [Biomacromolecules] 2020 Dec 14; Vol. 21 (12), pp. 4781-4794. Date of Electronic Publication: 2020 Nov 10.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't
اللغة:	English
بيانات الدورية:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 100892849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4602 (Electronic) Linking ISSN: 15257797 NLM ISO Abbreviation: Biomacromolecules Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Washington, DC : American Chemical Society, c2000-
مواضيع طبية MeSH:	Hydrogels* , Peptides*, Kinetics
مستخلص:	Self-assembling peptide-based hydrogels are a class of tunable soft materials that have been shown to be highly useful for a number of biomedical applications. The dynamic formation of the supramolecular fibrils that compose these materials has heretofore remained poorly characterized. A better understanding of this process would provide important insights into the behavior of these systems and could aid in the rational design of new peptide hydrogels. Here, we report the determination of the microscopic steps that underpin the self-assembly of a hydrogel-forming peptide, SgI 37-49 . Using theoretical models of linear polymerization to analyze the kinetic self-assembly data, we show that SgI 37-49 fibril formation is driven by fibril-catalyzed secondary nucleation and that all the microscopic processes involved in SgI 37-49 self-assembly display an enzyme-like saturation behavior. Moreover, this analysis allows us to quantify the rates of the underlying processes at different peptide concentrations and to calculate the time evolution of these reaction rates over the time course of self-assembly. We demonstrate here a new mechanistic approach for the study of self-assembling hydrogel-forming peptides, which is complementary to commonly used materials science characterization techniques.
المشرفين على المادة:	0 (Hydrogels) 0 (Peptides)
تواريخ الأحداث:	Date Created: 20201110 Date Completed: 20210618 Latest Revision: 20210618
رمز التحديث:	20231215
DOI:	10.1021/acs.biomac.0c00989
PMID:	33170649
قاعدة البيانات:	MEDLINE

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الوصف
تدمد:	1526-4602
DOI:	10.1021/acs.biomac.0c00989