دورية أكاديمية
أعرض في EDS

Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability.

التفاصيل البيبلوغرافية
العنوان: Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability.
المؤلفون: Booth JA; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway. james.booth@rr-research.no., Špírek M; Department of Biology, Masaryk University, Kamenice 5/A7, 625 00, Brno, Czech Republic.; International Clinical Research Center, Center for Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Pekarska 53, 656 91, Brno, Czech Republic., Lobie TA; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway., Skarstad K; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway., Krejci L; Department of Biology, Masaryk University, Kamenice 5/A7, 625 00, Brno, Czech Republic.; National Centre for Biomolecular Research, Masaryk University, Kamenice 5/A4, 625 00, Brno, Czech Republic.; International Clinical Research Center, Center for Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Pekarska 53, 656 91, Brno, Czech Republic., Bjørås M; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway. magnar.bjoras@rr-research.no.; Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway. magnar.bjoras@rr-research.no.
المصدر: Scientific reports [Sci Rep] 2020 Nov 10; Vol. 10 (1), pp. 19422. Date of Electronic Publication: 2020 Nov 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Anti-Bacterial Agents/*pharmacology , DNA Damage/*drug effects , DNA Damage/*genetics , Escherichia coli/*drug effects , Escherichia coli/*genetics , Genomic Instability/*drug effects , Genomic Instability/*genetics, DNA Damage/radiation effects ; Electrophoretic Mobility Shift Assay ; Escherichia coli/radiation effects ; Flow Cytometry ; Genomic Instability/radiation effects ; Hydrogen-Ion Concentration ; Microbial Viability/drug effects ; Microbial Viability/radiation effects ; Nalidixic Acid/pharmacology ; Propidium/pharmacology ; Rifampin/pharmacology ; Ultraviolet Rays
مستخلص: Extracellular pH has been assumed to play little if any role in how bacteria respond to antibiotics and antibiotic resistance development. Here, we show that the intracellular pH of Escherichia coli equilibrates to the environmental pH following treatment with the DNA damaging antibiotic nalidixic acid. We demonstrate that this allows the environmental pH to influence the transcription of various DNA damage response genes and physiological processes such as filamentation. Using purified RecA and a known pH-sensitive mutant variant RecA K250R we show how pH can affect the biochemical activity of a protein central to control of the bacterial DNA damage response system. Finally, two different mutagenesis assays indicate that environmental pH affects antibiotic resistance development. Specifically, at environmental pH's greater than six we find that mutagenesis plays a significant role in producing antibiotic resistant mutants. At pH's less than or equal to 6 the genome appears more stable but extensive filamentation is observed, a phenomenon that has previously been linked to increased survival in the presence of macrophages.
References: PLoS Biol. 2005 Jul;3(7):e255. (PMID: 16000023)
Curr Protoc Mol Biol. 2007 Jul;Chapter 1:Unit 1.17. (PMID: 18265391)
Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):2885-90. (PMID: 9501185)
Mol Syst Biol. 2006;2:2006.0008. (PMID: 16738554)
Mol Microbiol. 2008 Oct;70(1):258-70. (PMID: 18761622)
Gene. 1996;173(1 Spec No):33-8. (PMID: 8707053)
Urology. 2014 Sep;84(3):731.e1-7. (PMID: 25168568)
Mol Microbiol. 2005 Aug;57(4):1074-85. (PMID: 16091045)
Cell Mol Life Sci. 2009 Jan;66(1):82-93. (PMID: 18726173)
Anal Biochem. 2011 Jun 15;413(2):206-7. (PMID: 21362398)
J Biol Chem. 1990 Jul 15;265(20):11560-6. (PMID: 2142155)
Cell. 2007 Sep 7;130(5):797-810. (PMID: 17803904)
PLoS Biol. 2005 Jul;3(7):e238. (PMID: 15954802)
J Bacteriol. 2004 Aug;186(15):4838-43. (PMID: 15262914)
J Bacteriol. 2007 Aug;189(15):5601-7. (PMID: 17545292)
Crit Rev Biochem Mol Biol. 2010 Jun;45(3):171-84. (PMID: 20441441)
Nat Rev Microbiol. 2011 May;9(5):330-43. (PMID: 21464825)
Science. 2016 Dec 16;354(6318):. (PMID: 27980159)
Mol Cell. 2006 Jan 6;21(1):15-27. (PMID: 16387650)
Microbes Infect. 2011 May;13(5):426-37. (PMID: 21182979)
Cell. 2015 Jul 16;162(2):271-286. (PMID: 26186187)
Mol Microbiol. 1998 Apr;28(2):281-91. (PMID: 9622353)
J Biol Chem. 2008 Sep 5;283(36):24909-21. (PMID: 18603529)
BMC Biol. 2007 Mar 28;5:14. (PMID: 17391508)
PLoS Biol. 2010 Feb 23;8(2):e1000317. (PMID: 20186264)
J Mol Biol. 1993 Sep 5;233(1):59-66. (PMID: 8377192)
DNA Res. 2005;12(5):291-9. (PMID: 16769691)
Mol Biol Cell. 2014 Nov 1;25(21):3330-41. (PMID: 25165138)
J Mol Biol. 2008 Mar 14;377(1):148-61. (PMID: 18234215)
J Biol Chem. 1981 Aug 25;256(16):8835-44. (PMID: 7021553)
Genetics. 2001 May;158(1):41-64. (PMID: 11333217)
PLoS Genet. 2015 Dec 29;11(12):e1005757. (PMID: 26713761)
Science. 2004 Sep 10;305(5690):1629-31. (PMID: 15308764)
mBio. 2017 Feb 7;8(1):. (PMID: 28174313)
Proc Natl Acad Sci U S A. 2006 Dec 26;103(52):19884-9. (PMID: 17172451)
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. (PMID: 10829079)
Nat Rev Microbiol. 2016 Apr;14(5):320-30. (PMID: 27080241)
J Bacteriol. 1998 Aug;180(15):3946-53. (PMID: 9683493)
Nature. 2012 Nov 8;491(7423):274-8. (PMID: 23103864)
PLoS Biol. 2012;10(3):e1001281. (PMID: 22412352)
Genetics. 1991 Aug;128(4):695-701. (PMID: 1916241)
PLoS One. 2015 Jan 21;10(1):e0115611. (PMID: 25608006)
Gut. 2001 Apr;48(4):571-7. (PMID: 11247905)
Nat Rev Microbiol. 2008 Feb;6(2):162-8. (PMID: 18157153)
Nat Rev Microbiol. 2010 Mar;8(3):207-17. (PMID: 20157339)
J Biol Chem. 2009 Mar 20;284(12):7733-45. (PMID: 19129197)
Antimicrob Agents Chemother. 1975 Sep;8(3):251-61. (PMID: 1101818)
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6271-5. (PMID: 7031646)
Curr Top Med Chem. 2009;9(11):981-98. (PMID: 19747119)
Adv Microb Physiol. 2009;55:1-79, 317. (PMID: 19573695)
DNA Repair (Amst). 2016 Aug;44:42-50. (PMID: 27236212)
Mol Microbiol. 2000 Jul;37(2):410-23. (PMID: 10931335)
J Mol Biol. 1994 Aug 26;241(4):507-23. (PMID: 8057377)
Biochem Biophys Res Commun. 2003 May 2;304(2):279-84. (PMID: 12711311)
J Bacteriol. 2010 Sep;192(18):4694-700. (PMID: 20639336)
Nature. 1974 Jun 7;249(457):561-3. (PMID: 4599764)
PLoS One. 2011;6(7):e23126. (PMID: 21829590)
DNA Repair (Amst). 2011 Jun 10;10(6):567-76. (PMID: 21565563)
المشرفين على المادة: 0 (Anti-Bacterial Agents)
36015-30-2 (Propidium)
3B91HWA56M (Nalidixic Acid)
VJT6J7R4TR (Rifampin)
تواريخ الأحداث: Date Created: 20201111 Date Completed: 20210514 Latest Revision: 20210514
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7655802
DOI: 10.1038/s41598-020-76426-2
PMID: 33173044
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-020-76426-2