دورية أكاديمية
Bark extract of Cassia sieberiana DC. (Caesalpiniaceae) displayed good antibacterial activity against MDR gram-negative phenotypes in the presence of phenylalanine-arginine β-naphthylamide.
| العنوان: | Bark extract of Cassia sieberiana DC. (Caesalpiniaceae) displayed good antibacterial activity against MDR gram-negative phenotypes in the presence of phenylalanine-arginine β-naphthylamide. |
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| المؤلفون: | Ambadiang MMM; Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.; Department of Biochemistry, University of Yaounde 1, P.O. Box 812, Cameroun, Yaounde, Cameroon., Atontsa BCK; Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon., Tankeo SB; Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon., Nayim P; Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon., Wamba BEN; Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon., Bitchagno GTM; Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon.; Institute of Organic Chemistry, University of Mainz, Duesbergweg 10-14, D-55128, Mainz, Germany., Mpetga JDS; Department of Chemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon., Penlap VB; Department of Biochemistry, University of Yaounde 1, P.O. Box 812, Cameroun, Yaounde, Cameroon., Kuete V; Department of Biochemistry, University of Dschang, P.O. Box 67, Dschang, Cameroon. kuetevictor@yahoo.fr. |
| المصدر: | BMC complementary medicine and therapies [BMC Complement Med Ther] 2020 Nov 12; Vol. 20 (1), pp. 342. Date of Electronic Publication: 2020 Nov 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101761232 Publication Model: Electronic Cited Medium: Internet ISSN: 2662-7671 (Electronic) Linking ISSN: 26627671 NLM ISO Abbreviation: BMC Complement Med Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2020]- |
| مواضيع طبية MeSH: | Anti-Bacterial Agents/*pharmacology , Cassia/*chemistry , Dipeptides/*pharmacology , Gram-Negative Bacteria/*drug effects , Plant Extracts/*pharmacology, Anti-Bacterial Agents/chemistry ; Anti-Bacterial Agents/metabolism ; Bacterial Proteins/antagonists & inhibitors ; Bacterial Proteins/metabolism ; Drug Resistance, Multiple, Bacterial ; Gram-Negative Bacteria/growth & development ; Microbial Sensitivity Tests ; Plant Bark/chemistry ; Plant Extracts/chemistry ; Proton-Translocating ATPases/antagonists & inhibitors ; Proton-Translocating ATPases/metabolism |
| مستخلص: | Background: Multidrug-resistant (MDR) bacteria remain a major cause of morbidity and mortality globally. The present study was designed to investigate the in vitro antibacterial activities of crude methanol extract and constituents isolated by Column Chromatography (CC) from Cassia sieberiana bark (CSB) against ten MDR Gram-negative bacteria, as well as the mechanisms of action of the most active sample. Methods: The antibacterial activity of the tested samples (extract, the fractions and their compounds isolated by CC and the structures obtained by exploiting 1 H and 13 C Nuclear magnetic resonance (NMR) spectra) in the presence and absence of an efflux pumps inhibitor, phenylalanine-arginine β-naphthylamide (PAβN), was evaluated using the micro-dilution method. The effects of the most active sample were evaluated on the cell growth kinetic and on the bacterial H + -ATPase proton pumps. Results: Phytochemical composition of the crude extract showed a rather selective distribution of secondary metabolites (presence of polyphenols, tannins, steroids, triterpenes, flavonoids, alkaloids, saponins and absence of anthocyanins, anthraquinones). The tested samples displayed different antibacterial activities with minimal inhibitory concentrations (MICs) ranging from 64 to 512 μg/mL. Crude extract (CS) and fraction CSc showed the highest inhibitory spectra, both inhibiting all of the studied bacteria except Enterobacter aerogenes EA27 strain. Fraction CSc exerted bactericidal effects on most bacteria meanwhile, crude extract (CS) and sub-fraction CSc2 exerted bacteriostatic effects. Compounds 1 (spectaline) and 2 (iso-6-cassine) inhibited the growth of 70% (Escherichia coli ATCC8739 and AG102, Klebsiella pneumoniae ATCC11296, Enterobacter aerogenes ATCC13048 and EA27, Providencia stuartii ATCC29916, Pseudomonas aeruginosa PA01) and 60% (Escherichia coli ATCC8739, Klebsiella pneumoniae ATCC11296 and KP55, Providencia stuartii ATCC29916, Pseudomonas aeruginosa PA01 and PA124) of bacteria respectively with MICs ranging from 128 to 512 μg/mL. In the presence of PAβN, the activities of crude extract CS, fraction CAc and sub-fraction CSc2 strongly increased on most bacteria strains as their MICs significantly decreased. Sub-fraction CSc2 inhibited the H + -ATPase proton pumps and altered growth kinetic of Escherichia coli ATCC8739. Conclusion: The overall results justify the traditional use of C. sieberiana for the treatment of bacterial infections. |
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| فهرسة مساهمة: | Keywords: Cassia sieberiana; Efflux pumps; Gram negative bacteria; Infectious diseases; Multidrug resistance |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Bacterial Proteins) 0 (Dipeptides) 0 (Plant Extracts) 0 (phenylalanine arginine beta-naphthylamide) EC 3.6.3.14 (Proton-Translocating ATPases) |
| تواريخ الأحداث: | Date Created: 20201113 Date Completed: 20210721 Latest Revision: 20210721 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7664092 |
| DOI: | 10.1186/s12906-020-03148-3 |
| PMID: | 33183278 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2662-7671 |
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| DOI: | 10.1186/s12906-020-03148-3 |