دورية أكاديمية
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Construction of an artificial system for ambrein biosynthesis and investigation of some biological activities of ambrein.

التفاصيل البيبلوغرافية
العنوان: Construction of an artificial system for ambrein biosynthesis and investigation of some biological activities of ambrein.
المؤلفون: Yamabe Y; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan., Kawagoe Y; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan., Okuno K; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan., Inoue M; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan., Chikaoka K; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan., Ueda D; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan., Tajima Y; Department of Zoology, National Museum of Nature and Science, 4-1-1Amakubo, Tsukuba, Ibaraki, Japan., Yamada TK; Department of Zoology, National Museum of Nature and Science, 4-1-1Amakubo, Tsukuba, Ibaraki, Japan., Kakihara Y; Division of Dental Pharmacology, Department of Tissue Regeneration and Reconstruction, Faculty of Dentistry, Niigata University Graduate School of Medical and Dental Sciences, Chuo-ku, Niigata, 951-8514, Japan. kakihara@dent.niigata-u.ac.jp., Hara T; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan. harata@agr.niigata-u.ac.jp., Sato T; Department of Agriculture, Faculty of Agriculture, and Graduate School of Science and Technology, Niigata University, 8050, Ikarashi-2, Nishi-ku, Niigata, Japan. satot@agr.niigata-u.ac.jp.
المصدر: Scientific reports [Sci Rep] 2020 Nov 12; Vol. 10 (1), pp. 19643. Date of Electronic Publication: 2020 Nov 12.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Apoptosis*, Ambergris/*chemistry , Naphthols/*chemistry , Naphthols/*pharmacology , Osteoclasts/*cytology , Sperm Whale/*metabolism, Amyloid beta-Peptides/pharmacology ; Animals ; Cell Differentiation ; Cell Line ; Humans ; Osteoclasts/drug effects ; Osteoclasts/metabolism ; Triterpenes/chemistry ; Triterpenes/pharmacology ; Volatile Organic Compounds/chemistry ; Volatile Organic Compounds/pharmacology
مستخلص: Ambergris, a sperm whale metabolite, has long been used as a fragrance and traditional medication, but it is now rarely available. The odor components of ambergris result from the photooxidative degradation of the major component, ambrein. The pharmacological activities of ambergris have also been attributed to ambrein. However, efficient production of ambrein and odor compounds has not been achieved. Here, we constructed a system for the synthesis of ambrein and odor components. First, we created a new triterpene synthase, "ambrein synthase," for mass production of ambrein by redesigning a bacterial enzyme. The ambrein yields were approximately 20 times greater than those reported previously. Next, an efficient photooxidative conversion system from ambrein to a range of volatiles of ambergris was established. The yield of volatiles was 8-15%. Finally, two biological activities, promotion of osteoclast differentiation and prevention of amyloid β-induced apoptosis, were discovered using the synthesized ambrein.
References: Biochem Biophys Rep. 2015 Dec 30;5:253-258. (PMID: 28955831)
PLoS One. 2019 May 14;14(5):e0216729. (PMID: 31086389)
Chembiochem. 2017 Oct 5;18(19):1910-1913. (PMID: 28881085)
Biosci Biotechnol Biochem. 1999 Dec;63(12):2189-98. (PMID: 10664852)
J Nat Prod. 2007 Feb;70(2):147-53. (PMID: 17315955)
Org Lett. 2017 Feb 3;19(3):496-499. (PMID: 28102682)
Jpn J Pharmacol. 1992 Oct;60(2):67-71. (PMID: 1479744)
Nat Prod Res. 2019 Jul 30;:1-7. (PMID: 31359775)
Arch Int Pharmacodyn Ther. 1995 Mar-Apr;329(2):283-94. (PMID: 8540767)
Nat Prod Res. 2019 Nov;33(21):3134-3142. (PMID: 30470137)
J Am Chem Soc. 2011 Nov 9;133(44):17540-3. (PMID: 21981578)
J Am Chem Soc. 2013 Dec 11;135(49):18335-8. (PMID: 24274794)
J Bone Miner Res. 2015 May;30(5):837-54. (PMID: 25483983)
Biotechnol Lett. 2018 Feb;40(2):399-404. (PMID: 29204767)
Chembiochem. 2020 Oct 15;21(20):2931-2938. (PMID: 32495977)
Science. 2002 Jul 19;297(5580):353-6. (PMID: 12130773)
Sci Rep. 2017 Oct 2;7(1):12549. (PMID: 28970533)
Metab Eng Commun. 2018 Aug 16;7:e00077. (PMID: 30197866)
Yeast. 2020 Jan;37(1):163-172. (PMID: 31606910)
المشرفين على المادة: 0 (Ambergris)
0 (Amyloid beta-Peptides)
0 (Naphthols)
0 (Triterpenes)
0 (Volatile Organic Compounds)
1N9JB373FJ (ambrein)
تواريخ الأحداث: Date Created: 20201113 Date Completed: 20210325 Latest Revision: 20210325
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7661701
DOI: 10.1038/s41598-020-76624-y
PMID: 33184314
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-020-76624-y