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Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng .

التفاصيل البيبلوغرافية
العنوان: Metabolomes and transcriptomes revealed the saponin distribution in root tissues of Panax quinquefolius and Panax notoginseng .
المؤلفون: Wei G; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China., Yang F; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China., Wei F; Wenshan Miaoxiang Notoginseng Technology, Co., Ltd., Wenshan, China., Zhang L; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China., Gao Y; Institute of Desertification Studies, Chinese Academy of Forestry, Beijing, China., Qian J; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China., Chen Z; Institute of Sanqi Research, Wenshan University, Wenshan, China., Jia Z; Waters Corporation Shanghai Science & Technology Co Ltd, Shanghai, China., Wang Y; Institute of Sanqi Research, Wenshan University, Wenshan, China., Su H; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China., Dong L; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China., Xu J; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China., Chen S; Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
المصدر: Journal of ginseng research [J Ginseng Res] 2020 Nov; Vol. 44 (6), pp. 757-769. Date of Electronic Publication: 2019 May 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Korean Society of Ginseng Country of Publication: Korea (South) NLM ID: 100890690 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1226-8453 (Print) Linking ISSN: 12268453 NLM ISO Abbreviation: J Ginseng Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Sŏul : Korean Society of Ginseng, 1998-
مستخلص: Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants.
Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng , and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles.
Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng . Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents ( R  > 0.6, P  < 0.05) in the root tissues of P. quinquefolius and P. notoginseng , respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents of Rg1, Re, Rc, Rb2, and Rd in P. quinquefolius . UGT255 was markedly related to the content of R1; CYP74, CYP89, CYP100, CYP103, CYP109, and UGT190 were markedly correlated with the Rd content in P. notoginseng .
Conclusions: These results provided the visual and quantitative profiles of and confirmed the pivotal transcripts of CYPs and UGTs regulating the saponin distribution in the root tissues of P. quinquefolius and P. notoginseng .
(© 2019 The Korean Society of Ginseng. Publishing services by Elsevier B.V.)
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فهرسة مساهمة: Keywords: AACT, Acetoacetyl-CoA acyltransferase; DS, Dammarenediol-II synthase; DXPR, 1-deoxy-o-xylulose 5-phosphate reductoisomerase; DXPS, 1-deoxy-o-xylulose 5-phosphate synthase; FDR, False discovery rate; FPP, Farnesyl diphosphate; FPS, Farnesyl pyrophosphate synthase; GDPS, Gerenyl diphosphatesynthase; GO, Gene Ontology; HDS, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphatesynthase; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; HPLC-UV, High-performance liquid chromatography-ultraviolet detection; IPP, Isoprenyl diphosphate; IPPI, Isopentenyl pyrophosphate isomerase; ISPD, 2-C-methylerythritol 4-phosphatecytidyl transferase; ISPE, 4-(cytidine-5′-diphospho)-2-C-methylerythritol kinase; ISPH, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase; MALDI-MS, Matrix-assisted laser desorption/ionization–mass spectrometry; MECPS, 2-C-methylerythritol-2,4-cyclophosphate synthase; MEP, 2-C-methyl-D-erythritol-4-phosphate; MVA, Mevalonate acid; MVD, Mevalonate diphosphate decarboxylase; MVK, Mevalonate kinase; Metabolome; NCBI Nr, NCBI Non-redundant protein; OPLS-DA, Orthogonal partial least squares-discriminant analysis; ORF, Open read frame; P450, P450-monooxygenase; PMK, Phosphomevalonate kinase; Panax plants; Root tissues; SE, Squalene epoxidase; SS, Squalene synthase; Saponin distribution; Transcriptome; UGTs, UDP-glycosyltransferases; UPLC-MS, Ultrahigh-performance liquid chromatography quadrupole time of flight-mass spectrometry; WGCNA, Weighted gene coexpression network analysis
تواريخ الأحداث: Date Created: 20201116 Latest Revision: 20220418
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7655499
DOI: 10.1016/j.jgr.2019.05.009
PMID: 33192118
قاعدة البيانات: MEDLINE
الوصف
تدمد:1226-8453
DOI:10.1016/j.jgr.2019.05.009