دورية أكاديمية
Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes, Mi-flp1, Mi-flp12 , and Mi-flp18 Provides Resistance to Root-Knot Nematode, Meloidogyne incognita .
| العنوان: | Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes, Mi-flp1, Mi-flp12 , and Mi-flp18 Provides Resistance to Root-Knot Nematode, Meloidogyne incognita . |
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| المؤلفون: | Banakar P; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, India.; Department of Nematology and Centre for Bio-Nanotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, India., Hada A; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, India., Papolu PK; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, India., Rao U; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, India. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2020 Oct 23; Vol. 11, pp. 573916. Date of Electronic Publication: 2020 Oct 23 (Print Publication: 2020). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Root-knot nematode, Meloidogyne incognita , is a devastating sedentary endoparasite that causes considerable damage to agricultural crops worldwide. Modern approaches targeting the physiological processes have confirmed the potential of FMRFamide like peptide (FLPs) family of neuromotor genes for nematode management. Here, we assessed the knock down effect of Mi-flp1, Mi-flp12 , and Mi-flp18 of M. incognita and their combinatorial fusion cassette on infection and reproduction. Comparative developmental profiling revealed higher expression of all three FLPs in the infective 2nd stage juveniles (J2s). Further, Mi-flp1 expression in J2s could be localized in the ventral pharyngeal nerves near to metacarpal bulb of the central nervous system. In vitro RNAi silencing of three FLPs and their fusion cassette in M. incognita J2s showed that combinatorial silencing is the most effective and affected nematode host recognition followed by reduced penetration ability and subsequent infection into tomato and adzuki bean roots. Northern blot analysis of J2s soaked in fusion dsRNA revealed the presence of siRNA of all three target FLPs establishing successful processing of fusion gene dsRNA in the J2s. Further, evaluation of the fusion gene cassette is done through host-delivered RNAi in tobacco. Transgenic plants with fusion gene RNA-expressing vector were generated in which transgene integration was confirmed by PCR, qRT-PCR, and Southern blot analysis. Transcript accumulation of three FLPs constituting the fusion gene was reduced in the M. incognita females collected from the transgenic plants that provided additional evidence for successful gene silencing. Evaluation of positive T (Copyright © 2020 Banakar, Hada, Papolu and Rao.) |
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| فهرسة مساهمة: | Keywords: FMRFamide-like peptides; combinatorial RNAi; double-stranded RNA; neuromotor functions; root-knot nematodes; transgenic plants |
| تواريخ الأحداث: | Date Created: 20201116 Latest Revision: 20201117 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7644837 |
| DOI: | 10.3389/fmicb.2020.573916 |
| PMID: | 33193182 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2020.573916 |