دورية أكاديمية
On the road of dried blood spot sampling for antidoping tests: Detection of GHRP-2 abuse.
| العنوان: | On the road of dried blood spot sampling for antidoping tests: Detection of GHRP-2 abuse. |
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| المؤلفون: | Reverter-Branchat G; Integrative Pharmacology and Systems Neuroscience Research Group, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.; Department of Diabetes, Endocrinology, Nutritional Medicine and Metabolism, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland., Segura J; Integrative Pharmacology and Systems Neuroscience Research Group, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain.; Catalonian Antidoping Laboratory, Doping Control Research Group, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain., Pozo OJ; Integrative Pharmacology and Systems Neuroscience Research Group, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain. |
| المصدر: | Drug testing and analysis [Drug Test Anal] 2021 Mar; Vol. 13 (3), pp. 510-522. Date of Electronic Publication: 2020 Nov 25. |
| نوع المنشور: | Journal Article; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: England NLM ID: 101483449 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1942-7611 (Electronic) Linking ISSN: 19427603 NLM ISO Abbreviation: Drug Test Anal Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Chichester, UK : John Wiley & Sons |
| مواضيع طبية MeSH: | Doping in Sports/*prevention & control , Dried Blood Spot Testing/*methods , Oligopeptides/*analysis , Substance Abuse Detection/*methods, Administration, Intravenous ; Chromatography, Liquid/methods ; Humans ; Limit of Detection ; Oligopeptides/administration & dosage ; Oligopeptides/blood ; Tandem Mass Spectrometry/methods |
| مستخلص: | Dried blood spots (DBSs) sampling is gaining support by the antidoping community because of simplicity and cost-effective characteristics, especially in collection, transport, and storage. Nevertheless, DBS applicability demands specific studies for each of the analytes proposed for testing. Here, GHRP-2 has been selected as a representing member of the growth hormone-releasing peptides (GHRPs) family to provide further evidence of DBS suitability for GHRPs abuse detection in sport testing. An analytical procedure to extract GHRP-2 and its main metabolite (AA-3) from DBS and to detect them by liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed. The method has been validated for the detection of GHRP-2. Specificity and identification capabilities have been assessed in agreement with antidoping guidelines. The low AA-3 levels found in DBS samples prevented its effective application for the determination of this metabolite. The limit of detection (LoD) for GHRP-2 has been established at 50 pg/ml. Long-term stability (>2 years) has been confirmed. The procedure has been successfully applied to actual DBS samples from an administration study with a single intravenous dose of GHRP-2 (100 μg) being detected up to 4 h after drug injection. GHRP-2 concentrations have been higher in venous blood DBS than in capillary blood DBS. Despite the observed differences, a similar detection window has been achieved independently of the type of blood used. In summary, this study provides specific evidence supporting DBS usefulness to detect GHRP-2, and potentially other GHRPs family members, for antidoping tests. (© 2020 John Wiley & Sons Ltd.) |
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| فهرسة مساهمة: | Keywords: DBS; GHRP-2; doping; mass spectrometry |
| المشرفين على المادة: | 0 (Oligopeptides) E6S6E1F19M (growth hormone-releasing peptide-2) |
| تواريخ الأحداث: | Date Created: 20201116 Date Completed: 20211028 Latest Revision: 20211028 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/dta.2975 |
| PMID: | 33197153 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1942-7611 |
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| DOI: | 10.1002/dta.2975 |