دورية أكاديمية
أعرض في EDS

Fabrication of Bio-Based Gelatin Sponge for Potential Use as A Functional Acellular Skin Substitute.

التفاصيل البيبلوغرافية
العنوان: Fabrication of Bio-Based Gelatin Sponge for Potential Use as A Functional Acellular Skin Substitute.
المؤلفون: Arif MMA; Department of Physiology, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia., Fauzi MB; Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Jalan Yaacob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia., Nordin A; Department of Physiology, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia., Hiraoka Y; Biomaterial Group, R&D Center, Nitta Gelatin Inc. 2-22, Futamata, Yao City, Osaka 581-0024, Japan., Tabata Y; Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku Kyoto 606-8507, Japan., Yunus MHM; Department of Physiology, UKM Medical Centre, Jalan Yaacob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia.; Centre for Tissue Engineering and Regenerative Medicine, Faculty of Medicine, Jalan Yaacob Latiff, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia.
المصدر: Polymers [Polymers (Basel)] 2020 Nov 13; Vol. 12 (11). Date of Electronic Publication: 2020 Nov 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101545357 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4360 (Electronic) Linking ISSN: 20734360 NLM ISO Abbreviation: Polymers (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مستخلص: Gelatin possesses biological properties that resemble native skin and can potentially be fabricated as a skin substitute for full-thickness wound treatment. The native property of gelatin, whereby it is easily melted and degraded at body temperature, could prevent its biofunctionality for various applications. This study aimed to fabricate and characterise buffalo gelatin (Infanca halal certified) crosslinked with chemical type crosslinker (genipin and genipin fortified with EDC) and physicaly crosslink using the dihydrothermal (DHT) method. A porous gelatin sponge (GS) was fabricated by a freeze-drying process followed by a complete crosslinking via chemical-natural and synthetic-or physical intervention using genipin (GNP), 1-ethyl-3-(3-dimethylaminopropyl) (EDC) and dihydrothermal (DHT) methods, respectively. The physicochemical, biomechanical, cellular biocompatibility and cell-biomaterial interaction of GS towards human epidermal keratinocytes (HEK) and dermal fibroblasts (HDF) were evaluated. Results showed that GS had a uniform porous structure with pore size ranging between 60 and 200 µm with high porosity (>78.6 ± 4.1%), high wettability (<72.2 ± 7.0°), high tensile strain (>13.65 ± 1.10%) and 14 h of degradation rate. An increase in the concentration and double-crosslinking approach demonstrated an increment in the crosslinking degree, enzymatic hydrolysis resistance, thermal stability, porosity, wettability and mechanical strength. The GS can be tuned differently from the control by approaching the GS via a different crosslinking strategy. However, a decreasing trend was observed in the pore size, water retention and water absorption ability. Crosslinking with DHT resulted in large pore sizes (85-300 µm) and low water retention (236.9 ± 18.7 g/m 2 ·day) and a comparable swelling ratio with the control (89.6 ± 7.1%). Moreover no changes in the chemical content and amorphous phase identification were observed. The HEK and HDF revealed slight toxicity with double crosslinking. HEK and HDF attachment and proliferation remain similar to each crosslinking approach. Immunogenicity was observed to be higher in the double-crosslinking compared to the single-crosslinking intervention. The fabricated GS demonstrated a dynamic potential to be tailored according to wound types by manipulating the crosslinking intervention.
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معلومات مُعتمدة: GUP-2017-117 Universiti Kebangsaan Malaysia
فهرسة مساهمة: Keywords: biocompatibility; carbodiimide; gelatin sponge; genipin
تواريخ الأحداث: Date Created: 20201118 Latest Revision: 20201201
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7697907
DOI: 10.3390/polym12112678
PMID: 33202700
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4360
DOI:10.3390/polym12112678