دورية أكاديمية
أعرض في EDS

A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission.

التفاصيل البيبلوغرافية
العنوان: A combined transcriptomic approach to identify candidates for an anti-tick vaccine blocking B. afzelii transmission.
المؤلفون: Trentelman JJA; Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, Amsterdam UMC, Location Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. j.j.trentelman@amsterdamumc.nl., Sima R; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic., Krezdorn N; GenXPro GmbH, Frankfurt Innovation Center Biotechnology, Frankfurt am Main, Germany., Tomás-Cortázar J; CIC bioGUNE-Basque Research & Technology Alliance, 48160, Derio, Spain., Barriales D; CIC bioGUNE-Basque Research & Technology Alliance, 48160, Derio, Spain., Takumi K; National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands., Butler JM; Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, Amsterdam UMC, Location Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands., Sprong H; National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands., Klouwens MJ; Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, Amsterdam UMC, Location Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands., Urbanova V; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic., Mahmood S; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic.; Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic., Winter P; GenXPro GmbH, Frankfurt Innovation Center Biotechnology, Frankfurt am Main, Germany., Kopacek P; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic., Anguita J; CIC bioGUNE-Basque Research & Technology Alliance, 48160, Derio, Spain.; Ikerbasque, Basque Foundation for Science, 48012, Bilbao, Spain., Hajdusek O; Biology Centre, Institute of Parasitology, Czech Academy of Sciences, Ceske Budejovice, Czech Republic., Hovius JW; Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, Amsterdam UMC, Location Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
المصدر: Scientific reports [Sci Rep] 2020 Nov 18; Vol. 10 (1), pp. 20061. Date of Electronic Publication: 2020 Nov 18.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Transcriptome*, Arachnid Vectors/*microbiology , Arthropod Proteins/*genetics , Bacterial Vaccines/*administration & dosage , Lyme Disease/*genetics , Salivary Glands/*microbiology , Tick Infestations/*genetics, Animals ; Borrelia burgdorferi Group/drug effects ; Female ; Ixodes/drug effects ; Lyme Disease/microbiology ; Lyme Disease/prevention & control ; Lyme Disease/transmission ; Mice ; Tick Infestations/microbiology ; Tick Infestations/prevention & control ; Tick Infestations/transmission
مستخلص: Ixodes ricinus is the vector for Borrelia afzelii, the predominant cause of Lyme borreliosis in Europe, whereas Ixodes scapularis is the vector for Borrelia burgdorferi in the USA. Transcription of several I. scapularis genes changes in the presence of B. burgdorferi and contributes to successful infection. To what extend B. afzelii influences gene expression in I. ricinus salivary glands is largely unknown. Therefore, we measured expression of uninfected vs. infected tick salivary gland genes during tick feeding using Massive Analysis of cDNA Ends (MACE) and RNAseq, quantifying 26.179 unique transcripts. While tick feeding was the main differentiator, B. afzelii infection significantly affected expression of hundreds of transcripts, including 465 transcripts after 24 h of tick feeding. Validation of the top-20 B. afzelii-upregulated transcripts at 24 h of tick feeding in ten biological genetic distinct replicates showed that expression varied extensively. Three transcripts could be validated, a basic tail protein, a lipocalin and an ixodegrin, and might be involved in B. afzelii transmission. However, vaccination with recombinant forms of these proteins only marginally altered B. afzelii infection in I. ricinus-challenged mice for one of the proteins. Collectively, our data show that identification of tick salivary genes upregulated in the presence of pathogens could serve to identify potential pathogen-blocking vaccine candidates.
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المشرفين على المادة: 0 (Arthropod Proteins)
0 (Bacterial Vaccines)
تواريخ الأحداث: Date Created: 20201119 Date Completed: 20210330 Latest Revision: 20210330
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7674437
DOI: 10.1038/s41598-020-76268-y
PMID: 33208766
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-020-76268-y