| المؤلفون: |
Petrović K; Department of Agronomy, Horticulture, and Plant Science, South Dakota State University, Brookings, SD 57007, U.S.A.; Department of Soybean, Institute of Field and Vegetable Crops, Novi Sad 21000, Serbia., Skaltsas D; Mycology and Nematology Genetic Diversity and Biology Laboratory, U.S. Department of Agriculture Agricultural Research Service, Beltsville, MD 20705, U.S.A., Castlebury LA; Mycology and Nematology Genetic Diversity and Biology Laboratory, U.S. Department of Agriculture Agricultural Research Service, Beltsville, MD 20705, U.S.A., Kontz B; Department of Agronomy, Horticulture, and Plant Science, South Dakota State University, Brookings, SD 57007, U.S.A., Allen TW; Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776, U.S.A., Chilvers MI; Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824, U.S.A., Gregory N; Department of Plant and Soil Sciences, University of Delaware, Newark, DE 19716, U.S.A., Kelly HM; Department of Entomology and Plant Pathology, University of Tennessee, Knoxville, TN 37996, U.S.A., Koehler AM; Department of Plant and Soil Sciences, University of Delaware, Georgetown, DE 19947, U.S.A., Kleczewski NM; Department of Crop Sciences, University of Illinois, Urbana-Champaign, IL 61820, U.S.A., Mueller DS; Department of Plant Pathology and Microbiology, Iowa State University, Ames, IA 50011, U.S.A., Price PP 3rd; Department of Plant Pathology and Crop Physiology, Louisiana State University AgCenter, Winnsboro, LA 71295, U.S.A., Smith DL; Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, U.S.A., Mathew FM; Department of Agronomy, Horticulture, and Plant Science, South Dakota State University, Brookings, SD 57007, U.S.A. |
| مستخلص: |
Diaporthe seed decay can compromise seed quality in soybean [ Glycine max (L.) Merr.] in the warm and humid production areas of the United States during crop maturation. In the current study, 45 isolates of Diaporthe were recovered from seed sampled from soybean fields affected by Diaporthe -associated diseases in eight U.S. states in 2017. The isolates obtained belonged to 10 species of Diaporthe based on morphology and phylogenetic analyses of the internal transcribed spacer, partial translation elongation factor 1-α, and β-tubulin gene sequences. The associated species included D. aspalathi , D. caulivora , D. kongii , D. longicolla , D. sojae , D. ueckerae , D. unshiuensis , and three novel fungi, D. bacilloides , D. flavescens , and D. insulistroma . One isolate each of the 10 species was examined for pathogenicity on seed of cultivar Sava under controlled conditions. Seven days postinoculation, significant differences in the percentages of decayed seeds and seedling necrosis were observed among the isolates and the noninoculated control ( P < 0.0001). While the isolates of D. bacilloides , D. longicolla , and D. ueckerae caused a significantly greater percentage of decayed seeds ( P < 0.0001), the isolate of D. aspalathi caused the greatest seedling necrosis ( P < 0.0001). The observation of new fungi causing Diaporthe seed decay suggests the need for a more comprehensive survey in U.S. soybean producing areas since members of the genus Diaporthe appear to form a complex that causes seed decay. |
