دورية أكاديمية
Colonization of carbapenem-resistant Klebsiella pneumoniae in a sink-drain model biofilm system.
| العنوان: | Colonization of carbapenem-resistant Klebsiella pneumoniae in a sink-drain model biofilm system. |
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| المؤلفون: | Burgos-Garay M; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia., Ganim C; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia., de Man TJB; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia., Davy T; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia., Mathers AJ; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia., Kotay S; Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia Health System, Charlottesville, Virginia., Daniels J; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia., Perry KA; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia., Breaker E, Donlan RM; Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia. |
| المصدر: | Infection control and hospital epidemiology [Infect Control Hosp Epidemiol] 2021 Jun; Vol. 42 (6), pp. 722-730. Date of Electronic Publication: 2020 Nov 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cambridge University Press Country of Publication: United States NLM ID: 8804099 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-6834 (Electronic) Linking ISSN: 0899823X NLM ISO Abbreviation: Infect Control Hosp Epidemiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2015- : Cambridge : Cambridge University Press Original Publication: [Thorofare, N.J. ] : SLACK Inc., c1988- |
| مواضيع طبية MeSH: | Carbapenem-Resistant Enterobacteriaceae* , Klebsiella pneumoniae*/genetics, Biofilms ; Carbapenems/pharmacology ; Humans ; RNA, Ribosomal, 16S |
| مستخلص: | Background: Sink drains in healthcare facilities may provide an environment for antimicrobial-resistant microorganisms, including carbapenemase-producing Klebsiella pneumoniae (CPKP). Methods: We investigated the colonization of a biofilm consortia by CPKP in a model system simulating a sink-drain P-trap. Centers for Disease Control (CDC) biofilm reactors (CBRs) were inoculated with microbial consortia originally recovered from 2 P-traps collected from separate patient rooms (designated rooms A and B) in a hospital. Biofilms were grown on stainless steel (SS) or polyvinyl chloride (PVC) coupons in autoclaved municipal drinking water (ATW) for 7 or 28 days. Results: Microbial communities in model systems (designated CBR-A or CBR-B) were less diverse than communities in respective P-traps A and B, and they were primarily composed of β and γ Proteobacteria, as determined using 16S rRNA community analysis. Following biofilm development CBRs were inoculated with either K. pneumoniae ST45 (ie, strain CAV1016) or K. pneumoniae ST258 KPC+ (ie, strain 258), and samples were collected over 21 days. Under most conditions tested (CBR-A: SS, 7-day biofilm; CBR-A: PVC, 28-day biofilm; CBR-B: SS, 7-day and 28-day biofilm; CBR-B: PVC, 28-day biofilm) significantly higher numbers of CAV1016 were observed compared to 258. CAV1016 showed no significant difference in quantity or persistence based on biofilm age (7 days vs 28 days) or substratum type (SS vs PVC). However, counts of 258 were significantly higher on 28-day biofilms and on SS. Conclusions: These results suggest that CPKP persistence in P-trap biofilms may be strain specific or may be related to the type of P-trap material or age of the biofilm. |
| المشرفين على المادة: | 0 (Carbapenems) 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20201125 Date Completed: 20210906 Latest Revision: 20210906 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1017/ice.2020.1287 |
| PMID: | 33234179 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1559-6834 |
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| DOI: | 10.1017/ice.2020.1287 |