دورية أكاديمية
أعرض في EDS

miR‑18a‑5p promotes melanoma cell proliferation and inhibits apoptosis and autophagy by targeting EPHA7 signaling.

التفاصيل البيبلوغرافية
العنوان: miR‑18a‑5p promotes melanoma cell proliferation and inhibits apoptosis and autophagy by targeting EPHA7 signaling.
المؤلفون: Guo Y; Department of Dermatology, School of Medicine, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China., Shi W; Department of Dermatology, School of Medicine, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China., Fang R; Department of Dermatology, School of Medicine, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China.
المصدر: Molecular medicine reports [Mol Med Rep] 2021 Jan; Vol. 23 (1). Date of Electronic Publication: 2020 Nov 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Athens, Greece : D. A. Spandidos
مواضيع طبية MeSH: Apoptosis* , Autophagy* , Cell Proliferation* , Signal Transduction*, Melanoma/*metabolism , MicroRNAs/*metabolism , Neoplasm Proteins/*metabolism , RNA, Neoplasm/*metabolism , Receptor, EphA7/*metabolism , Skin Neoplasms/*metabolism, Cell Line, Tumor ; Female ; Humans ; Male ; Melanoma/genetics ; Melanoma/pathology ; MicroRNAs/genetics ; Neoplasm Proteins/genetics ; RNA, Neoplasm/genetics ; Receptor, EphA7/genetics ; Skin Neoplasms/genetics ; Skin Neoplasms/pathology
مستخلص: Micro (mi)RNAs serve crucial roles in cancer development although little is known about their cellular mechanisms in the pathogenesis of melanoma. The present study explored the regulatory roles of miR‑18a‑5p in melanoma cell proliferation, apoptosis and autophagy, in addition to its target gene in melanoma cells. miRNA and ephrin receptor A7 (EPHA7) mRNA were analyzed by reverse transcription‑quantitative PCR. Cell Counting Kit‑8 and colony formation assays were performed to examine the cell proliferation rate. Hoechst staining and flow cytometry were performed to investigate cell apoptosis. Western blotting was used to estimate the abundance of proteins. Dual-luciferase reporter assay verified the binding of miRNA with target gene sequences. Melanoma tissues and cell lines exhibited markedly elevated miR‑18a‑5p expression. miR‑18a‑5p inhibitor inhibited proliferation rates, and triggered apoptosis and autophagy marker protein expression in WM266‑4 and A375 cells. It also negatively regulated EPHA7 expression in WM266‑4 and A375 cells by directly binding at the 3'‑untranslated region of EPHA7. miR‑18a‑5p mimics reversed the EPHA7 overexpression‑induced suppression of proliferation, and the EPHA7 overexpression‑induced promotion of apoptosis and autophagy. miR‑18a‑5p triggered proliferation of melanoma cells and inhibited apoptosis and autophagy by directly targeting and inhibiting EPHA7 expression. Thus, the present study aided our understanding of miRNA‑mediated melanoma pathogenesis.
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فهرسة مساهمة: Keywords: miR‑18a‑5p; ephrin receptor A7; melanoma; proliferation; apoptosis; autophagy
المشرفين على المادة: 0 (MIRN18A microRNA, human)
0 (MicroRNAs)
0 (Neoplasm Proteins)
0 (RNA, Neoplasm)
EC 2.7.10.1 (Receptor, EphA7)
تواريخ الأحداث: Date Created: 20201125 Date Completed: 20210505 Latest Revision: 20210630
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7716404
DOI: 10.3892/mmr.2020.11717
PMID: 33236144
قاعدة البيانات: MEDLINE
الوصف
تدمد:1791-3004
DOI:10.3892/mmr.2020.11717