دورية أكاديمية
miR‑18a‑5p promotes melanoma cell proliferation and inhibits apoptosis and autophagy by targeting EPHA7 signaling.
العنوان: | miR‑18a‑5p promotes melanoma cell proliferation and inhibits apoptosis and autophagy by targeting EPHA7 signaling. |
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المؤلفون: | Guo Y; Department of Dermatology, School of Medicine, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China., Shi W; Department of Dermatology, School of Medicine, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China., Fang R; Department of Dermatology, School of Medicine, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, Guangdong 510180, P.R. China. |
المصدر: | Molecular medicine reports [Mol Med Rep] 2021 Jan; Vol. 23 (1). Date of Electronic Publication: 2020 Nov 25. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: D. A. Spandidos Country of Publication: Greece NLM ID: 101475259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-3004 (Electronic) Linking ISSN: 17912997 NLM ISO Abbreviation: Mol Med Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Athens, Greece : D. A. Spandidos |
مواضيع طبية MeSH: | Apoptosis* , Autophagy* , Cell Proliferation* , Signal Transduction*, Melanoma/*metabolism , MicroRNAs/*metabolism , Neoplasm Proteins/*metabolism , RNA, Neoplasm/*metabolism , Receptor, EphA7/*metabolism , Skin Neoplasms/*metabolism, Cell Line, Tumor ; Female ; Humans ; Male ; Melanoma/genetics ; Melanoma/pathology ; MicroRNAs/genetics ; Neoplasm Proteins/genetics ; RNA, Neoplasm/genetics ; Receptor, EphA7/genetics ; Skin Neoplasms/genetics ; Skin Neoplasms/pathology |
مستخلص: | Micro (mi)RNAs serve crucial roles in cancer development although little is known about their cellular mechanisms in the pathogenesis of melanoma. The present study explored the regulatory roles of miR‑18a‑5p in melanoma cell proliferation, apoptosis and autophagy, in addition to its target gene in melanoma cells. miRNA and ephrin receptor A7 (EPHA7) mRNA were analyzed by reverse transcription‑quantitative PCR. Cell Counting Kit‑8 and colony formation assays were performed to examine the cell proliferation rate. Hoechst staining and flow cytometry were performed to investigate cell apoptosis. Western blotting was used to estimate the abundance of proteins. Dual-luciferase reporter assay verified the binding of miRNA with target gene sequences. Melanoma tissues and cell lines exhibited markedly elevated miR‑18a‑5p expression. miR‑18a‑5p inhibitor inhibited proliferation rates, and triggered apoptosis and autophagy marker protein expression in WM266‑4 and A375 cells. It also negatively regulated EPHA7 expression in WM266‑4 and A375 cells by directly binding at the 3'‑untranslated region of EPHA7. miR‑18a‑5p mimics reversed the EPHA7 overexpression‑induced suppression of proliferation, and the EPHA7 overexpression‑induced promotion of apoptosis and autophagy. miR‑18a‑5p triggered proliferation of melanoma cells and inhibited apoptosis and autophagy by directly targeting and inhibiting EPHA7 expression. Thus, the present study aided our understanding of miRNA‑mediated melanoma pathogenesis. |
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فهرسة مساهمة: | Keywords: miR‑18a‑5p; ephrin receptor A7; melanoma; proliferation; apoptosis; autophagy |
المشرفين على المادة: | 0 (MIRN18A microRNA, human) 0 (MicroRNAs) 0 (Neoplasm Proteins) 0 (RNA, Neoplasm) EC 2.7.10.1 (Receptor, EphA7) |
تواريخ الأحداث: | Date Created: 20201125 Date Completed: 20210505 Latest Revision: 20210630 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC7716404 |
DOI: | 10.3892/mmr.2020.11717 |
PMID: | 33236144 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1791-3004 |
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DOI: | 10.3892/mmr.2020.11717 |