دورية أكاديمية
أعرض في EDS

Synthetic peptides against Trichophyton mentagrophytes and T. rubrum: Mechanisms of action and efficiency compared to griseofulvin and itraconazole.

التفاصيل البيبلوغرافية
العنوان: Synthetic peptides against Trichophyton mentagrophytes and T. rubrum: Mechanisms of action and efficiency compared to griseofulvin and itraconazole.
المؤلفون: Lima PG; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil., Souza PFN; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil. Electronic address: pedrofilhobio@gmail.com., Freitas CDT; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil., Bezerra LP; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil., Neto NAS; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil., Silva AFB; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil., Oliveira JTA; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil., Sousa DOB; Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, Ceará CEP 60.440-554, Brazil. Electronic address: daniele.sousa@ufc.br.
المصدر: Life sciences [Life Sci] 2021 Jan 15; Vol. 265, pp. 118803. Date of Electronic Publication: 2020 Nov 23.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Amsterdam : Elsevier
Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press.
مواضيع طبية MeSH: Antifungal Agents/*pharmacology , Arthrodermataceae/*drug effects , Griseofulvin/*pharmacology , Itraconazole/*pharmacology , Peptide Fragments/*pharmacology, Antifungal Agents/chemical synthesis ; Arthrodermataceae/physiology ; Chemistry Techniques, Synthetic ; Griseofulvin/chemical synthesis ; Humans ; Itraconazole/chemical synthesis ; Peptide Fragments/chemical synthesis
مستخلص: Aims: According to the WHO, 20-25% of people worldwide are affected by skin infections caused by dermatophytes, such as those of the Trichophyton genus. Additionally, several dermatophytes have developed resistance to drugs such as griseofulvin and itraconazole. This study tested 2S albumins-derived antimicrobial peptides (AMPs) as alternative antidermatophytic molecules.
Main Methods: Membrane pore formation assays, tests to detect overproduction of ROS, scanning electron microscopy (SEM) and fluorescence microscopy (FM) were carried out to provide insight into the mechanisms of antidermatophytic action.
Key Findings: All AMPs (at 50 μg mL -1 ) tested reduced the mycelial growth of T. mentagrophytes and T. rubrum by up to 95%. In contrast, using a concentration 20-fold higher, griseofulvin only inhibited T. mentagrophytes by 35%, while itraconazole was not active against both dermatophytes. Scanning electron and fluorescence microscopies revealed that the six AMPs caused severe damage to hyphal morphology by inducing cell wall rupture, hyphal content leakage, and death. Peptides also induced membrane pore formation and oxidative stress by overproduction of ROS. Based on the stronger activity of peptides than the commercial drugs and the mechanism of action, all six peptides have the potential to be either employed as models to develop new antidermatophytic drugs or as adjuvants to existing ones.
Significance: The synthetic peptides are more efficient than conventional drug to treat infection caused by dermatophytes being potential molecules to develop new drugs.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Dermatophytes; Griseofulvin; Itraconazole; Synthetic antifungal peptides; Synthetic peptides
المشرفين على المادة: 0 (Antifungal Agents)
0 (Peptide Fragments)
304NUG5GF4 (Itraconazole)
32HRV3E3D5 (Griseofulvin)
SCR Organism: Trichophyton mentagrophytes; Trichophyton rubrum
تواريخ الأحداث: Date Created: 20201125 Date Completed: 20210113 Latest Revision: 20210113
رمز التحديث: 20221213
DOI: 10.1016/j.lfs.2020.118803
PMID: 33238167
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0631
DOI:10.1016/j.lfs.2020.118803