دورية أكاديمية
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First-time application of a PCR-based clonality assay in a large cohort of non-domestic felines.

التفاصيل البيبلوغرافية
العنوان: First-time application of a PCR-based clonality assay in a large cohort of non-domestic felines.
المؤلفون: Unterkreuter S; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria., Posautz A; Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Sciences, University of Veterinary Medicine Vienna, Austria., Rütgen BC; Clinical Pathology Unit, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria., Groiss S; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria., Kübber-Heiss A; Research Institute of Wildlife Ecology, Department of Interdisciplinary Life Sciences, University of Veterinary Medicine Vienna, Austria., Hammer SE; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Austria. Electronic address: sabine.hammer@vetmeduni.ac.at.
المصدر: Research in veterinary science [Res Vet Sci] 2021 Mar; Vol. 135, pp. 511-516. Date of Electronic Publication: 2020 Nov 19.
نوع المنشور: Journal Article; Validation Study
اللغة: English
بيانات الدورية: Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: London : British Veterinary Association
Original Publication: Oxford.
مواضيع طبية MeSH: Felidae*, Lymphoma/*veterinary , Polymerase Chain Reaction/*veterinary, Animals ; Cohort Studies ; Female ; Gene Rearrangement ; Lymphocytes ; Lymphoma/diagnosis ; Lymphoma/genetics ; Male ; Receptors, Antigen, T-Cell, gamma-delta ; Sensitivity and Specificity
مستخلص: Feline lymphoma, one of the most important malignant tumors in domestic cats, is also increasingly diagnosed in non-domestic felines, most notably, African lions (Panthera leo). The gold standard for the diagnosis of lymphoma is histopathological evaluation. As an additional tool, the PCR for antigen receptor gene rearrangement (PARR) has been established. To support the diagnosis on a molecular level, the PCR-based clonality assay is designed to distinguish between reactive and neoplastic lymphocyte populations. In general, PARR primers are used to target complete immunoglobulin heavy chain V-D-J (IGH-VDJ) and T-cell receptor gamma V-J (TRG-VJ) chain gene rearrangements. In this study, we validated the primer sets used in routine diagnostics of domestic cats for the application in non-domestic felines. Clonality testing was used in 41 non-domestic feline species and the results were interpreted in the light of their clinical history and their pathology. In total, clonality could be detected in 8 non-domestic felines (19.4%), including 3 lymphoma cases confirmed by histopathology. These results confirmed the successful application of domestic feline-specific PARR primers in non-domestic feline species. Diagnostic sensitivity and specificity of the clonality assay were 100% and 88%, respectively. Finally, the overall diagnostic accuracy was 89%.
(Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
فهرسة مساهمة: Keywords: Antigen receptor gene rearrangement; Clonality testing; Feline; Immunoglobulin heavy chain; Lymphoma; PARR; T-cell receptor gamma chain
المشرفين على المادة: 0 (Receptors, Antigen, T-Cell, gamma-delta)
تواريخ الأحداث: Date Created: 20201127 Date Completed: 20210524 Latest Revision: 20210524
رمز التحديث: 20240628
DOI: 10.1016/j.rvsc.2020.11.011
PMID: 33243452
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-2661
DOI:10.1016/j.rvsc.2020.11.011