دورية أكاديمية
أعرض في EDS

Supranutritional selenium level minimizes high concentrate diet-induced epithelial injury by alleviating oxidative stress and apoptosis in colon of goat.

التفاصيل البيبلوغرافية
العنوان: Supranutritional selenium level minimizes high concentrate diet-induced epithelial injury by alleviating oxidative stress and apoptosis in colon of goat.
المؤلفون: Samo SP; Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060, Tando Jam, Pakistan., Malhi M; Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060, Tando Jam, Pakistan. mcmalhi@sau.edu.pk., Kachiwal AB; Department Veterinary Physiology and Biochemistry, Sindh Agricultural University, 70060, Tando Jam, Pakistan., Gadahi JA; Department of Veterinary Parasitology, Sindh Agricultural University, 70060, Tandojam, Pakistan., Parveen F; Department of Veterinary Pathology, Sindh Agricultural University, 70060, Tandojam, Pakistan., Kalhoro NH; Sindh Poultry Vaccine Centre, Animal Science Complex, Korangi , 74900, Karachi, Pakistan., Lei Y; Dairy Herd Improvement Center, Henan Animal Husbandry Bureau, 450046, Zhengzhou, China.
المصدر: BMC veterinary research [BMC Vet Res] 2020 Nov 27; Vol. 16 (1), pp. 462. Date of Electronic Publication: 2020 Nov 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Animal Feed/*adverse effects , Goats/*physiology , Selenium/*administration & dosage, Animal Feed/analysis ; Animals ; Apoptosis/drug effects ; Colon/drug effects ; Diet/veterinary ; Fatty Acids, Volatile/metabolism ; Female ; Intestinal Mucosa/drug effects ; Malondialdehyde/metabolism ; Oxidative Stress/drug effects
مستخلص: Background: High concentrate (HC) diet-induced oxidative stress causes gut epithelial damages associated with apoptosis. Selenium (Se) being an integral component of glutathione peroxidase (GSH-Px) plays an important role in antioxidant defense system. Therefore, increasing dietary Se level would alleviate HC diet-induced injuries in gut mucosa. The present study investigated eighteen cross-bred goats, randomly divided into three groups (n = 6/group) fed either low concentrate (LC, roughage: concentrate ratio 65:35), high concentrate (HC, 35:65) or HC plus Se (HC-SY) diets for 10 weeks. Se was supplemented at the dose rate of 0.5 mg Se kg - 1 diet in the form of selenium yeast. The background Se level in HC and LC diets were 0.15 and 0.035 mg.kg - 1 diet, respectively. The Se at the dose of 0.115 mg.kg - 1 diet was added in LC diet to make its concentration equivalent to HC diet and with the supplementation of 0.5 mg Se kg - 1 , the goats in group HC-SY received total Se by 0.65 mg.kg - 1 diet.
Results: The molar concentrations of individual and total short chain fatty acids (TSCFA) significantly increased (P < 0.05) with simultaneous decrease in pH of colonic fluid in goats of HC and HC-SY groups compared with LC goats. HC diet induced loss of epithelial integrity, inflammation and loss of goblet cells in colonic mucosa associated with higher lipopolysaccharide (LPS) concentrations in colonic fluid whereas, the addition of SY in HC diet alleviated such damaging changes. Compared with LC, the HC diet elevated malondialdehyde (MDA) level with concurrent decrease in GSH-Px and superoxide dismutase (SOD) activities, while SY supplementation attenuated these changes and improved antioxidant status in colonic epithelium. Moreover, epithelial injury and oxidative stress in colon of HC goats were associated with increased apoptosis as evidenced by downregulation of bcl2 and upregulation of bax, caspases 3 and 8 mRNA expressions compared with LC goats. On contrary, addition of SY in HC (HC-SY) diet alleviated these changes by modulating expression of apoptotic genes in colonic epithelium.
Conclusions: Our data suggest that supranutritional level of Se attenuates HC diet-induced oxidative stress and apoptosis and thereby minimizes the epithelial injury in colon of goats.
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فهرسة مساهمة: Keywords: apoptosis; goat colon; high concentrate diet; oxidative stress; selenium
المشرفين على المادة: 0 (Fatty Acids, Volatile)
4Y8F71G49Q (Malondialdehyde)
H6241UJ22B (Selenium)
تواريخ الأحداث: Date Created: 20201128 Date Completed: 20210528 Latest Revision: 20210528
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7694315
DOI: 10.1186/s12917-020-02653-4
PMID: 33246474
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-020-02653-4