دورية أكاديمية
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Utilization of low-molecular-weight organic compounds by the filterable fraction of a lotic microbiome.

التفاصيل البيبلوغرافية
العنوان: Utilization of low-molecular-weight organic compounds by the filterable fraction of a lotic microbiome.
المؤلفون: Ghuneim LJ; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK., Distaso MA; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.; Centre for Environmental Biotechnology, Bangor University, Bangor, Gwynedd, LL57 2UW, UK., Chernikova TN; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.; Centre for Environmental Biotechnology, Bangor University, Bangor, Gwynedd, LL57 2UW, UK., Bargiela R; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.; Centre for Environmental Biotechnology, Bangor University, Bangor, Gwynedd, LL57 2UW, UK., Lunev EA; Institute of Living Systems, Immanuel Kant Baltic Federal University, Kaliningrad, Russia., Korzhenkov AA; Kurchatov Center for Genome Research, National Research Center 'Kurchatov Institute', Moscow, Russian Federation., Toshchakov SV; Winogradsky Institute of Microbiology, FRC Biotechnology, Russian Academy of Sciences, Moscow, Russian Federation., Rojo D; Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad CEU San Pablo, Campus Montepríncipe, Madrid, Spain., Barbas C; Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad CEU San Pablo, Campus Montepríncipe, Madrid, Spain., Ferrer M; Institute of Catalysis, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain., Golyshina OV; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.; Centre for Environmental Biotechnology, Bangor University, Bangor, Gwynedd, LL57 2UW, UK., Golyshin PN; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.; Centre for Environmental Biotechnology, Bangor University, Bangor, Gwynedd, LL57 2UW, UK., Jones DL; School of Natural Sciences, Bangor University, Bangor, Gwynedd, LL57 2UW, UK.; UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia.
المصدر: FEMS microbiology ecology [FEMS Microbiol Ecol] 2021 Feb 05; Vol. 97 (2).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 8901229 Publication Model: Print Cited Medium: Internet ISSN: 1574-6941 (Electronic) Linking ISSN: 01686496 NLM ISO Abbreviation: FEMS Microbiol Ecol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2015- : Oxford Oxford University Press
Original Publication: [Amsterdam] : Elsevier Science Publishers on behalf of the Federation of European Microbiological Societies, [1985-
مواضيع طبية MeSH: Microbiota* , Organic Chemicals*, Carbon ; Fresh Water ; RNA, Ribosomal, 16S/genetics ; Rivers
مستخلص: Filterable microorganisms participate in dissolved organic carbon (DOC) cycling in freshwater systems, however their exact functional role remains unknown. We determined the taxonomic identity and community dynamics of prokaryotic microbiomes in the 0.22 µm-filtered fraction and unfiltered freshwater from the Conwy River (North Wales, UK) in microcosms and, using targeted metabolomics and 14C-labelling, examined their role in the utilization of amino acids, organic acids and sugars spiked at environmentally-relevant (nanomolar) concentrations. To identify changes in community structure, we used 16S rRNA amplicon and shotgun sequencing. Unlike the unfiltered water samples where the consumption of DOC was rapid, the filtered fraction showed a 3-day lag phase before the consumption started. Analysis of functional categories of clusters of orthologous groups of proteins (COGs) showed that COGs associated with energy production increased in number in both fractions with substrate addition. The filtered fraction utilized low-molecular-weight (LMW) DOC at much slower rates than the whole community. Addition of nanomolar concentrations of LMW DOC did not measurably influence the composition of the microbial community nor the rate of consumption across all substrate types in either fraction. We conclude that due to their low activity, filterable microorganisms play a minor role in LMW DOC processing within a short residence time of lotic freshwater systems.
(© The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.)
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فهرسة مساهمة: Keywords: 14C-radioisotope tracking; 16S rRNA amplicon sequencing; dissolved organic matter (DOM); filterable microorganisms; freshwater; metabolomics; microbial ecology; shotgun sequencing
المشرفين على المادة: 0 (Organic Chemicals)
0 (RNA, Ribosomal, 16S)
7440-44-0 (Carbon)
تواريخ الأحداث: Date Created: 20201202 Date Completed: 20210420 Latest Revision: 20210420
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7864478
DOI: 10.1093/femsec/fiaa244
PMID: 33264383
قاعدة البيانات: MEDLINE
الوصف
تدمد:1574-6941
DOI:10.1093/femsec/fiaa244