دورية أكاديمية
Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles.
| العنوان: | Semi-Solid Pharmaceutical Formulations for the Delivery of Papain Nanoparticles. |
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| المؤلفون: | Lima CSA; Nuclear and Energy Research Institute, University of São Paulo, São Paulo 05508-000, Brazil., Varca JPRO; Nuclear and Energy Research Institute, University of São Paulo, São Paulo 05508-000, Brazil., Nogueira KM; Nuclear and Energy Research Institute, University of São Paulo, São Paulo 05508-000, Brazil., Fazolin GN; Nuclear and Energy Research Institute, University of São Paulo, São Paulo 05508-000, Brazil., Freitas LF; Nuclear and Energy Research Institute, University of São Paulo, São Paulo 05508-000, Brazil., Souza EW; Department of Polymers, Technology College (Fatec), São Paulo 03694-000, Brazil., Lugão AB; Nuclear and Energy Research Institute, University of São Paulo, São Paulo 05508-000, Brazil., Varca GHC; Nuclear and Energy Research Institute, University of São Paulo, São Paulo 05508-000, Brazil. |
| المصدر: | Pharmaceutics [Pharmaceutics] 2020 Dec 01; Vol. 12 (12). Date of Electronic Publication: 2020 Dec 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101534003 Publication Model: Electronic Cited Medium: Print ISSN: 1999-4923 (Print) Linking ISSN: 19994923 NLM ISO Abbreviation: Pharmaceutics Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI |
| مستخلص: | Papain is a therapeutic enzyme with restricted applications due to associated allergenic reactions. Papain nanoparticles have shown to be safe for biomedical use, although a method for proper drug loading and release remains to be developed. Thus, the objective of this work was to develop and assess the stability of papain nanoparticles in a prototype semi-solid formulation suitable for dermatological or topical administrations. Papain nanoparticles of 7.0 ± 0.1 nm were synthesized and loaded into carboxymethylcellulose- and poly(vinyl alcohol)-based gels. The formulations were then assayed for preliminary stability, enzyme activity, cytotoxicity studies, and characterized according to their microstructures and protein distribution. The formulations were suitable for papain nanoparticle loading and provided a stable environment for the nanoparticles. The enzyme distribution along the gel matrix was homogeneous for all the formulations, and the proteolytic activity was preserved after the gel preparation. Both gels presented a slow release of the papain nanoparticles for four days. Cell viability assays revealed no potential cytotoxicity, and the presence of the nanoparticles did not alter the microstructure of the gel. The developed systems presented a potential for biomedical applications, either as drug delivery systems for papain nanoparticles and/or its complexes. |
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| معلومات مُعتمدة: | 2019/01315-1 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2015/13979-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; CRPF 22064 International Atomic Energy Agency; 402887/2013-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 442527/2019-5 Sistema Nacional de Laboratórios em Nanotecnologias (SisNano) |
| فهرسة مساهمة: | Keywords: gel; nanopapain; nanoparticles; semi-solid formulation; therapeutic enzymes |
| تواريخ الأحداث: | Date Created: 20201204 Latest Revision: 20201226 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7761214 |
| DOI: | 10.3390/pharmaceutics12121170 |
| PMID: | 33271859 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1999-4923 |
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| DOI: | 10.3390/pharmaceutics12121170 |