دورية أكاديمية
Ultra-high-throughput SPE-MALDI workflow: Blueprint for efficient purification and screening of peptide libraries.
| العنوان: | Ultra-high-throughput SPE-MALDI workflow: Blueprint for efficient purification and screening of peptide libraries. |
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| المؤلفون: | Bennett R; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA. Electronic address: raffeal.bennett@merck.com., Pirrone GF; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA. Electronic address: gregory.pirrone@merck.com., Nowak T; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA., Mukherjee D; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA., Shchurik V; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA., Mapelli C; Merck & Co., Inc., MRL, Chemistry Capabilities for Accelerating Therapeutics, Discovery Chemistry, Kenilworth, NJ, 07033, USA., Hickey JL; Merck & Co., Inc., MRL, Chemistry Capabilities for Accelerating Therapeutics, Discovery Chemistry, Kenilworth, NJ, 07033, USA., Regalado EL; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA. Electronic address: erik.regalado@merck.com., Mangion I; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA., Makarov AA; Merck & Co., Inc., MRL, Analytical Research & Development, Rahway, NJ, 07065, USA. |
| المصدر: | Analytica chimica acta [Anal Chim Acta] 2021 Jan 15; Vol. 1142, pp. 10-18. Date of Electronic Publication: 2020 Oct 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Amsterdam. |
| مواضيع طبية MeSH: | Peptide Library* , Peptides*, High-Throughput Screening Assays ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Workflow |
| مستخلص: | At the forefront of synthetic endeavors in the pharmaceutical industry, including drug discovery and high-throughput screening, timelines are tight and large quantities of pure chemical targets are rarely available. In this regard, the development of novel and increasingly challenging chemistries requires a commensurate level of innovation to develop reliable analytical assays and purification workflows with rapid turnaround that enables accelerated pharmacological evaluation. A small-scale automation platform enabling high-throughput analysis and purification to streamline the selection of candidate leads would be a transformative advance. Herein, we introduce an automation-friendly solid-phase extraction-matrix-assisted laser desorption/ionization (SPE-MALDI) platform applied to the high-throughput purification and analysis of peptide libraries. This advance enabled us to purify peptides from microgram levels in less than a day with results comparable to traditional high-performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS). Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: High-throughput analysis; High-throughput screening; MALDI-MS; Peptide; Resin screening; Solid-phase extraction; mRNA display |
| المشرفين على المادة: | 0 (Peptide Library) 0 (Peptides) |
| تواريخ الأحداث: | Date Created: 20201207 Date Completed: 20210514 Latest Revision: 20210514 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.aca.2020.10.045 |
| PMID: | 33280687 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4324 |
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| DOI: | 10.1016/j.aca.2020.10.045 |