دورية أكاديمية
أعرض في EDS

Scalar nanostructure of the Candida albicans cell wall; a molecular, cellular and ultrastructural analysis and interpretation.

التفاصيل البيبلوغرافية
العنوان: Scalar nanostructure of the Candida albicans cell wall; a molecular, cellular and ultrastructural analysis and interpretation.
المؤلفون: Lenardon MD; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK., Sood P; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK., Dorfmueller HC; Molecular Microbiology, School of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland, UK., Brown AJP; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK., Gow NAR; Aberdeen Fungal Group, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK.
المصدر: Cell surface (Amsterdam, Netherlands) [Cell Surf] 2020 Nov 08; Vol. 6, pp. 100047. Date of Electronic Publication: 2020 Nov 08 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101728565 Publication Model: eCollection Cited Medium: Internet ISSN: 2468-2330 (Electronic) Linking ISSN: 24682330 NLM ISO Abbreviation: Cell Surf Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier, [2018]-
مستخلص: Despite the importance of fungal cell walls as the principle determinant of fungal morphology and the defining element determining fungal interactions with other cells, few scalar models have been developed that reconcile chemical and microscopic attributes of its structure. The cell wall of the fungal pathogen Candida albicans is comprised of an amorphous inner skeletal layer of β(1,3)- and β(1,6)-glucan and chitin and an outer fibrillar layer thought to be dominated by highly mannosylated cell wall proteins. The architecture of these two layers can be resolved at the electron microscopy level, but the visualised structure of the wall has not yet been defined precisely in chemical terms. We have therefore examined the precise structure, location and molecular sizes of the cell wall components using transmission electron microscopy and tomography and tested predictions of the cell wall models using mutants and agents that perturb the normal cell wall structure. We demonstrate that the fibrils are comprised of a frond of N -linked outer chain mannans linked to a basal layer of GPI-proteins concentrated in the mid-wall region and that the non-elastic chitin microfibrils are cantilevered with sufficient lengths of non-fibrillar chitin and/or β-glucan to enable the chitin-glucan cage to flex, e.g. during morphogenesis and osmotic swelling. We present the first three-dimensional nano-scalar model of the C. albicans cell wall which can be used to test hypotheses relating to the structure-function relationships that underpin the pathobiology of this fungal pathogen.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2020 The Authors.)
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معلومات مُعتمدة: MR/N006364/2 United Kingdom MRC_ Medical Research Council; MR/J008230/1 United Kingdom MRC_ Medical Research Council; MR/M026663/1 United Kingdom MRC_ Medical Research Council; MR/N006364/1 United Kingdom MRC_ Medical Research Council; MR/M026663/2 United Kingdom MRC_ Medical Research Council; 109357/Z/15/Z United Kingdom WT_ Wellcome Trust
فهرسة مساهمة: Keywords: 2D, two dimensions; 2°, secondary; 3D, three dimensions; 3°, tertiary; 6xHis, hexahistidine tag; AFM, atomic force microscopy; BSA, bovine serum albumin; CWPs, cell wall proteins; Cell wall proteins; ChBD, chitin binding domain; Chitin; EndoH, endoglycosidase H; Fc-dectin-1, soluble chimeric form of dectin-1; Fungal cell wall ultrastructure; GPI, glycosylphosphatidylinositol; HPF/FS, high pressure freezing/freeze substitution; HuCκ, human kappa light chain; N-mannan; NMR, nuclear magnetic resonance; OD600, optical density at 600 nm; PAMPs, pathogen associated molecular patterns; PBS, phosphate buffered saline; PRRs, pattern recognition receptors; SEM, scanning electron microscopy; TEM, transmission electron microscopy; WGA, wheat germ agglutinin; rpm, revolutions per minute; scAb, single chain antibody; β-glucan
تواريخ الأحداث: Date Created: 20201209 Latest Revision: 20240306
رمز التحديث: 20240306
مُعرف محوري في PubMed: PMC7691183
DOI: 10.1016/j.tcsw.2020.100047
PMID: 33294751
قاعدة البيانات: MEDLINE
الوصف
تدمد:2468-2330
DOI:10.1016/j.tcsw.2020.100047