دورية أكاديمية
Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar.
| العنوان: | Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar. |
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| المؤلفون: | Moe Sann WW; Medical Technology Program, Faculty of Associated medical Sciences, Khon Kaen University, Khon Kaen,Thailand. w.waingms@gmail.com., Namwat W; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand. wisnam@kku.ac.th., Faksri K; Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand. kiatichai@kku.ac.th., Swe TL; National TB Control Program, Yangon, Myanmar. thynleiswe@gmail.com., Swe KK; National TB Control Program, Upper Myanmar TB Reference Laboratory, Mandalay Region, Myanmar. drkyikyiswe1986@gmail.com., Thwin T; National TB Control Program, Upper Myanmar TB Reference Laboratory, Mandalay Region, Myanmar. drthandarthwin1996@gmail.com., Sangka A; Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand. arusan@kku.ac.th. |
| المصدر: | Journal of infection in developing countries [J Infect Dev Ctries] 2020 Nov 30; Vol. 14 (11), pp. 1296-1305. Date of Electronic Publication: 2020 Nov 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Open Learning on Enteric Pathogens Country of Publication: Italy NLM ID: 101305410 Publication Model: Electronic Cited Medium: Internet ISSN: 1972-2680 (Electronic) Linking ISSN: 19722680 NLM ISO Abbreviation: J Infect Dev Ctries Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Italy?] : Open Learning on Enteric Pathogens |
| مواضيع طبية MeSH: | Genetic Variation*, Bacterial Typing Techniques/*methods , Minisatellite Repeats/*genetics , Molecular Typing/*methods , Mycobacterium tuberculosis/*classification , Mycobacterium tuberculosis/*genetics, Adolescent ; Adult ; Aged ; Cluster Analysis ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Myanmar ; Phylogeny ; Tuberculosis/microbiology ; Tuberculosis/transmission ; Young Adult |
| مستخلص: | Introduction: MIRU-VNTR typing and Spoligotyping are the useful molecular tools for TB epidemiology study. Information regarding genetic diversity and tuberculosis (TB) transmission in Upper Myanmar only is scares. Methodology: We determined the genetic diversity of Mycobacterium tuberculosis (Mtb) and TB transmission from Upper Myanmar TB Reference Laboratory, Mandalay Region, including Mandalay (72), Shan (22), Magway (15), Sagaing (13), Nay Pyi Taw (8), Kachin (7), Chin (2) and Kayah (1). One hundred and forty Mtb isolates were genotyped using 24-locus MIRU-VNTR typing and spoligotyping. Lineage classification and TB transmission analysis were performed. Results: 24-locus MIRU-VNTR typing identified 135 unique profiles and two clusters compared to 35 spoligotyping profiles which contained 12 clusters and 23 unique isolates, Beijing (n=100, 71.4%) was found to be prominent lineage by combine two methods. The expected proportion attributable to recent transmission based on clustering rate was 2.1%. One cluster case was more likely to be in MDR patient. Conclusions: Our findings showed Beijing genotypes were dominant in Upper Myanmar. The usage and analysis of 24-locus MIRU-VNTR typing might prove useful for our broader understanding of TB outbreaks and epidemiology than spoligotyping. The genotypic pattern of this combined method suggests that the lower transmission rate may be due to a higher possibility of reactivation cases in Upper Myanmar. Competing Interests: No Conflict of Interest is declared (Copyright (c) 2020 Waing Waing Moe Sann, Wises Namwat, Kiatichai Faksri, Thyn Lei Swe, Kyi Kyi Swe, Thandar Thwin, Arunnee Sangka.) |
| فهرسة مساهمة: | Keywords: Beijing; HGDI; Lineages; MIRU-VNTR; spoligotyping |
| تواريخ الأحداث: | Date Created: 20201209 Date Completed: 20210902 Latest Revision: 20210902 |
| رمز التحديث: | 20231215 |
| DOI: | 10.3855/jidc.12998 |
| PMID: | 33296343 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1972-2680 |
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| DOI: | 10.3855/jidc.12998 |