دورية أكاديمية
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro.
| العنوان: | Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro. |
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| المؤلفون: | Soares MF; Department of Surgery and Animal Reproduction, São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil., Melo LM; Department of Surgery and Animal Reproduction, São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil., Bragato JP; Department of Surgery and Animal Reproduction, São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil., Furlan AO; Department of Support, Production and Animal Health, São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil., Scaramele NF; Department of Support, Production and Animal Health, São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil., Lopes FL; Department of Support, Production and Animal Health, São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil., Lima VMF; Department of Surgery and Animal Reproduction, São Paulo State University (UNESP), School of Veterinary Medicine, Araçatuba, São Paulo, Brazil. Electronic address: valeria.lima@unesp.br. |
| المصدر: | Research in veterinary science [Res Vet Sci] 2021 Jan; Vol. 134, pp. 58-63. Date of Electronic Publication: 2020 Dec 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: British Veterinary Association Country of Publication: England NLM ID: 0401300 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2661 (Electronic) Linking ISSN: 00345288 NLM ISO Abbreviation: Res Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : British Veterinary Association Original Publication: Oxford. |
| مواضيع طبية MeSH: | Leishmania infantum*, Dog Diseases/*parasitology , Leishmaniasis, Visceral/*veterinary , Leukocytes, Mononuclear/*metabolism , MicroRNAs/*metabolism, Animals ; Cells, Cultured ; Dog Diseases/blood ; Dog Diseases/genetics ; Dog Diseases/immunology ; Dogs ; Down-Regulation ; Leishmaniasis, Visceral/blood ; Leishmaniasis, Visceral/genetics ; Leishmaniasis, Visceral/immunology ; MAP Kinase Signaling System |
| مستخلص: | Visceral Leishmaniasis (VL) is a neglected tropical disease, caused by L. infantum in the New World, where dogs are the main reservoir. These parasites can regulate host immune response through miRNA differential expression in the early stages of infection; however such early response has not yet been investigated in the canine model. PBMC from healthy dogs were exposed to L. infantum in vitro and microarray analysis showed an upregulation of miR-206, miR-302d, miR-433, miR-214, miR-493, miR-514, miR-1835, miR-210, miR-539, miR-432, miR-188, miR-345 and downregulation of miR-489 and miR-503 in comparison to non-exposed control cells, at 24 h post-exposure. In silico target prediction showed that the upregulated miRNAs target 1541 genes, which can modulate important pathways involved in the early immune responses, like the "MAPK signaling pathway", one of the most relevant pathways to Leishmania survival inside host cells. These findings shed light on parasite modulation of host immunity following Leishmania infection, which in turn can be explored for drug development. (Copyright © 2020. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Dog; Leishmania; MAPK; miRNA |
| المشرفين على المادة: | 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20201210 Date Completed: 20210322 Latest Revision: 20210322 |
| رمز التحديث: | 20240513 |
| DOI: | 10.1016/j.rvsc.2020.11.021 |
| PMID: | 33302213 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2661 |
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| DOI: | 10.1016/j.rvsc.2020.11.021 |