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Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.

التفاصيل البيبلوغرافية
العنوان: Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse.
المؤلفون: Mota TR; Department of Biochemistry, State University of Maringá, UEM, Maringá, Paraná, 87020-900, Brazil., Oliveira DM; Department of Biochemistry, State University of Maringá, UEM, Maringá, Paraná, 87020-900, Brazil., Simister R; Centre for Novel Agricultural Products, Department of Biology, CNAP, University of York, York YO10 5DD, United Kingdom., Whitehead C; Centre for Novel Agricultural Products, Department of Biology, CNAP, University of York, York YO10 5DD, United Kingdom., Lanot A; Centre for Novel Agricultural Products, Department of Biology, CNAP, University of York, York YO10 5DD, United Kingdom., Dos Santos WD; Department of Biochemistry, State University of Maringá, UEM, Maringá, Paraná, 87020-900, Brazil., Rezende CA; Institute of Chemistry, University of Campinas, UNICAMP, Campinas, São Paulo 13083‑970, Brazil., McQueen-Mason SJ; Centre for Novel Agricultural Products, Department of Biology, CNAP, University of York, York YO10 5DD, United Kingdom. Electronic address: simon.mcqueen-mason@york.ac.uk., Gomez LD; Centre for Novel Agricultural Products, Department of Biology, CNAP, University of York, York YO10 5DD, United Kingdom. Electronic address: leonardo.gomez@york.ac.uk.
المصدر: Bioresource technology [Bioresour Technol] 2021 Feb; Vol. 321, pp. 124499. Date of Electronic Publication: 2020 Dec 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
مواضيع طبية MeSH: Saccharum*, Cellulose ; Hydrolysis ; Lignin
مستخلص: To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1-1 mol/L, temperature: 100-220 °C, and time: 20-80 min) and enzymatic saccharification (enzyme loading: 2.5-17.5%, and reaction volume: 550-850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25-110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
التعليقات: Erratum in: Bioresour Technol. 2021 Apr;325:124763. (PMID: 33516595)
فهرسة مساهمة: Keywords: Alkaline pretreatment; Biomass pretreatment; Central composite orthogonal design; Fractional factorial design; Lignocellulose
المشرفين على المادة: 9004-34-6 (Cellulose)
9005-53-2 (Lignin)
9006-97-7 (bagasse)
تواريخ الأحداث: Date Created: 20201214 Date Completed: 20201229 Latest Revision: 20210131
رمز التحديث: 20240628
DOI: 10.1016/j.biortech.2020.124499
PMID: 33310387
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2976
DOI:10.1016/j.biortech.2020.124499