دورية أكاديمية
ARHGAP21 Acts as an Inhibitor of the Glucose-Stimulated Insulin Secretion Process.
| العنوان: | ARHGAP21 Acts as an Inhibitor of the Glucose-Stimulated Insulin Secretion Process. |
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| المؤلفون: | Ferreira SM; Obestity and Comorbidities Research Center/Biology Institute, University State of Campinas (UNICAMP), Campinas, Brazil., Costa-Júnior JM; Obestity and Comorbidities Research Center/Biology Institute, University State of Campinas (UNICAMP), Campinas, Brazil., Kurauti MA; Departament Physiological Sciences, University State of Maringá (UEM), Maringá, Brazil., Leite NC; Obestity and Comorbidities Research Center/Biology Institute, University State of Campinas (UNICAMP), Campinas, Brazil., Ortis F; Department of Cellular Biology and Development, Institute of Biomedical Sciences, University State of São Paulo (USP), São Paulo, Brazil., Rezende LF; Departament of Physiopathology, University State of Montes Claros (UNIMONTES), Montes Claros, Brazil., Barbosa HC; Obestity and Comorbidities Research Center/Biology Institute, University State of Campinas (UNICAMP), Campinas, Brazil., Boschero AC; Obestity and Comorbidities Research Center/Biology Institute, University State of Campinas (UNICAMP), Campinas, Brazil., Santos GJ; Departament of Physiological Sciences, Center for Biological Sciences, University Federal of Santa Catarina (UFSC), Florianópolis, Brazil. |
| المصدر: | Frontiers in endocrinology [Front Endocrinol (Lausanne)] 2020 Nov 26; Vol. 11, pp. 599165. Date of Electronic Publication: 2020 Nov 26 (Print Publication: 2020). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101555782 Publication Model: eCollection Cited Medium: Print ISSN: 1664-2392 (Print) Linking ISSN: 16642392 NLM ISO Abbreviation: Front Endocrinol (Lausanne) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Lausanne : Frontiers Research Foundation] |
| مواضيع طبية MeSH: | Insulin Secretion*, GTPase-Activating Proteins/*physiology , Gene Expression Regulation/*drug effects , Glucose/*pharmacology , Hyperinsulinism/*prevention & control , Insulin-Secreting Cells/*drug effects, Animals ; Homeostasis ; Hyperinsulinism/metabolism ; Hyperinsulinism/pathology ; Insulin-Secreting Cells/metabolism ; Insulin-Secreting Cells/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Sweetening Agents/pharmacology |
| مستخلص: | ARHGAP21 is a RhoGAP protein implicated in the modulation of insulin secretion and energy metabolism. ARHGAP21 transient-inhibition increase glucose-stimulated insulin secretion (GSIS) in neonatal islets; however, ARHGAP21 heterozygote mice have a reduced insulin secretion. These discrepancies are not totally understood, and it might be related to functional maturation of beta cells and peripheral sensitivity. Here, we investigated the real ARHGAP21 role in the insulin secretion process using an adult mouse model of acute ARHGAP21 inhibition, induced by antisense. After ARHGAP21 knockdown induction by antisense injection in 60-day old male mice, we investigated glucose and insulin tolerance test, glucose-induced insulin secretion, glucose-induced intracellular calcium dynamics, and gene expression. Our results showed that ARHGAP21 acts negatively in the GSIS of adult islet. This effect seems to be due to the modulation of important points of insulin secretion process, such as the energy metabolism (PGC1α), Ca 2+ signalization (SYTVII), granule-extrusion (SNAP25), and cell-cell interaction (CX36). Therefore, based on these finds, ARHGAP21 may be an important target in Diabetes Mellitus (DM) treatment. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2020 Ferreira, Costa-Júnior, Kurauti, Leite, Ortis, Rezende, Barbosa, Boschero and Santos.) |
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| فهرسة مساهمة: | Keywords: ARHGAP21; Ob/Ob mice; calcium influx; insulin secretion; type 2 diabetes |
| المشرفين على المادة: | 0 (Arhgap21 protein, mouse) 0 (GTPase-Activating Proteins) 0 (Sweetening Agents) IY9XDZ35W2 (Glucose) |
| تواريخ الأحداث: | Date Created: 20201216 Date Completed: 20210603 Latest Revision: 20210603 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7726208 |
| DOI: | 10.3389/fendo.2020.599165 |
| PMID: | 33324349 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-2392 |
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| DOI: | 10.3389/fendo.2020.599165 |