التفاصيل البيبلوغرافية
العنوان: |
SARS-CoV-2 RNA reverse-transcribed and integrated into the human genome. |
المؤلفون: |
Zhang L, Richards A, Khalil A, Wogram E, Ma H, Young RA, Jaenisch R |
المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2020 Dec 13. Date of Electronic Publication: 2020 Dec 13. |
نوع المنشور: |
Preprint |
اللغة: |
English |
بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
مستخلص: |
Prolonged SARS-CoV-2 RNA shedding and recurrence of PCR-positive tests have been widely reported in patients after recovery, yet these patients most commonly are non-infectious. Here we investigated the possibility that SARS-CoV-2 RNAs can be reverse-transcribed and integrated into the human genome and that transcription of the integrated sequences might account for PCR-positive tests. In support of this hypothesis, we found chimeric transcripts consisting of viral fused to cellular sequences in published data sets of SARS-CoV-2 infected cultured cells and primary cells of patients, consistent with the transcription of viral sequences integrated into the genome. To experimentally corroborate the possibility of viral retro-integration, we describe evidence that SARS-CoV-2 RNAs can be reverse transcribed in human cells by reverse transcriptase (RT) from LINE-1 elements or by HIV-1 RT, and that these DNA sequences can be integrated into the cell genome and subsequently be transcribed. Human endogenous LINE-1 expression was induced upon SARS-CoV-2 infection or by cytokine exposure in cultured cells, suggesting a molecular mechanism for SARS-CoV-2 retro-integration in patients. This novel feature of SARS-CoV-2 infection may explain why patients can continue to produce viral RNA after recovery and suggests a new aspect of RNA virus replication. |
معلومات مُعتمدة: |
T32 EB016652 United States EB NIBIB NIH HHS |
تواريخ الأحداث: |
Date Created: 20201217 Latest Revision: 20220209 |
رمز التحديث: |
20240628 |
مُعرف محوري في PubMed: |
PMC7743078 |
DOI: |
10.1101/2020.12.12.422516 |
PMID: |
33330870 |
قاعدة البيانات: |
MEDLINE |