دورية أكاديمية
Effects of biperiden (cholinergic muscarinic m1/m4 receptor antagonist) on ethanol conditioned place preference in mice.
| العنوان: | Effects of biperiden (cholinergic muscarinic m1/m4 receptor antagonist) on ethanol conditioned place preference in mice. |
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| المؤلفون: | Palombo P; Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil., Engi SA; Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil., Yokoyama TS; Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil., Bezerra AG; Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil., Curado DF; Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil., Anésio A; Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil., Leão RM; Laboratório de Farmacologia, Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia - UFU, Uberlândia, Brazil., Santos PCJL; Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil., Cruz FC; Departamento de Farmacologia, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address: f.cruz@unifesp.br., Galduróz JCF; Departamento de Psicobiologia, Universidade Federal de São Paulo, São Paulo, Brazil. |
| المصدر: | Neuroscience letters [Neurosci Lett] 2021 Feb 06; Vol. 745, pp. 135551. Date of Electronic Publication: 2020 Dec 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Publishers Ireland Country of Publication: Ireland NLM ID: 7600130 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7972 (Electronic) Linking ISSN: 03043940 NLM ISO Abbreviation: Neurosci Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Scientific Publishers Ireland Original Publication: Amsterdam, Elsevier/North-Holland. |
| مواضيع طبية MeSH: | Biperiden/*administration & dosage , Conditioning, Classical/*drug effects , Ethanol/*administration & dosage , Muscarinic Antagonists/*administration & dosage , Receptor, Muscarinic M1/*antagonists & inhibitors , Receptor, Muscarinic M4/*antagonists & inhibitors, Animals ; Conditioning, Classical/physiology ; Dopamine/metabolism ; Dose-Response Relationship, Drug ; Homovanillic Acid/metabolism ; Injections, Intraperitoneal ; Male ; Mice ; Nucleus Accumbens/drug effects ; Nucleus Accumbens/metabolism ; Receptor, Muscarinic M1/metabolism ; Receptor, Muscarinic M4/metabolism |
| مستخلص: | Background: Previous studies suggest that muscarinic cholinergic receptors might act upon the dopamine release in the mesolimbic system and alter drug-reinforcing values related to drug craving. Aims: We examined the effects of systemic biperiden administration, a muscarinic cholinergic (M1/M4) receptor antagonist, on ethanol (dose of 2 g/Kg) conditioned place preference (CPP), neuronal activation, dopamine and its metabolites levels in the nucleus accumbens. Methods: Thirty minutes before the ethanol-induced CPP test, mice received saline or biperiden at doses of 1.0, 5.0, or 10.0 mg/kg. The time spent in each compartment was recorded for 15 min. After the CPP protocol, animals were euthanized, and we investigated the activation of the nucleus accumbens by immunohistochemistry for Fos. We also quantified dopamine, homovanillic acid (HVA), and dihydroxyphenylacetic acid (DOPAC) levels in the nucleus accumbens by high-performance liquid chromatography (HPLC). Additionally, the rotarod was employed to evaluate the effects of biperiden on motor coordination. Results: Biperiden at different doses (1.0, 5.0, and 10.0 mg/kg) blocked the expression of ethanol-induced CPP. These biperiden doses increased the number of Fos-positive cells and the dopamine turnover in the nucleus accumbens. None of the doses affected the motor coordination evaluated by the rotarod. Conclusions: Our results show that biperiden can modulate the effect of alcohol reward, and its mechanism of action may involve a change in dopamine and cholinergic mesolimbic neurotransmission. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Biperiden; Conditioned place preference; Dopamine turnover; Ethanol; Fos; Muscarinic receptor antagonist |
| المشرفين على المادة: | 0 (Muscarinic Antagonists) 0 (Receptor, Muscarinic M1) 0 (Receptor, Muscarinic M4) 0FRP6G56LD (Biperiden) 3K9958V90M (Ethanol) VTD58H1Z2X (Dopamine) X77S6GMS36 (Homovanillic Acid) |
| تواريخ الأحداث: | Date Created: 20201221 Date Completed: 20210429 Latest Revision: 20210429 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.neulet.2020.135551 |
| PMID: | 33346074 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7972 |
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| DOI: | 10.1016/j.neulet.2020.135551 |