دورية أكاديمية
DENT-seq for genome-wide strand-specific identification of DNA single-strand break sites with single-nucleotide resolution.
| العنوان: | DENT-seq for genome-wide strand-specific identification of DNA single-strand break sites with single-nucleotide resolution. |
|---|---|
| المؤلفون: | Elacqua JJ; MIT Department of Biological Engineering, Cambridge, Massachusetts 02139, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA., Ranu N; MIT Department of Biological Engineering, Cambridge, Massachusetts 02139, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA., DiIorio SE; MIT Department of Biological Engineering, Cambridge, Massachusetts 02139, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA., Blainey PC; MIT Department of Biological Engineering, Cambridge, Massachusetts 02139, USA.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.; Koch Institute for Integrative Cancer Research at MIT, Cambridge, Massachusetts 02142, USA. |
| المصدر: | Genome research [Genome Res] 2021 Jan; Vol. 31 (1), pp. 75-87. Date of Electronic Publication: 2020 Dec 21. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9518021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-5469 (Electronic) Linking ISSN: 10889051 NLM ISO Abbreviation: Genome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c1995- |
| مواضيع طبية MeSH: | DNA Repair*/genetics, DNA ; Endonucleases/metabolism ; Gene Editing ; Nucleotides |
| مستخلص: | DNA single-strand breaks (SSBs), or "nicks," are the most common form of DNA damage. Oxidative stress, endogenous enzyme activities, and other processes cause tens of thousands of nicks per cell per day. Accumulation of nicks, caused by high rates of occurrence or defects in repair enzymes, has been implicated in multiple diseases. However, improved methods for nick analysis are needed to characterize the mechanisms of these processes and learn how the location and number of nicks affect cells, disease progression, and health outcomes. In addition to natural processes, including DNA repair, leading genome editing technologies rely on nuclease activity, including nick generation, at specific target sites. There is currently a pressing need for methods to study off-target nicking activity genome-wide to evaluate the side effects of emerging genome editing tools on cells and organisms. Here, we developed a new method, DENT-seq, for efficient strand-specific profiling of nicks in complex DNA samples with single-nucleotide resolution and low false-positive rates. DENT-seq produces a single deep sequence data set enriched for reads near nick sites and establishes a readily detectable mutational signal that allows for determination of the nick site and strand with single-base resolution at penetrance as low as one strand per thousand. We apply DENT-seq to profile the off-target activity of the Nb.BsmI nicking endonuclease and an engineered spCas9 nickase. DENT-seq will be useful in exploring the activity of engineered nucleases in genome editing and other biotechnological applications as well as spontaneous and therapeutic-associated strand breaks. (© 2021 Elacqua et al.; Published by Cold Spring Harbor Laboratory Press.) |
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| معلومات مُعتمدة: | DP2 HL141005 United States HL NHLBI NIH HHS |
| المشرفين على المادة: | 0 (Nucleotides) 9007-49-2 (DNA) EC 3.1.- (Endonucleases) |
| تواريخ الأحداث: | Date Created: 20201223 Date Completed: 20211215 Latest Revision: 20240806 |
| رمز التحديث: | 20240806 |
| مُعرف محوري في PubMed: | PMC7849381 |
| DOI: | 10.1101/gr.265223.120 |
| PMID: | 33355294 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1549-5469 |
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| DOI: | 10.1101/gr.265223.120 |