دورية أكاديمية
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Overexpression of SARS-CoV-2 protein ORF6 dislocates RAE1 and NUP98 from the nuclear pore complex.

التفاصيل البيبلوغرافية
العنوان: Overexpression of SARS-CoV-2 protein ORF6 dislocates RAE1 and NUP98 from the nuclear pore complex.
المؤلفون: Kato K; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan., Ikliptikawati DK; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan., Kobayashi A; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan. Electronic address: akoba@staff.kanazawa-u.ac.jp., Kondo H; Division of Transdisciplinary Sciences, Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan., Lim K; WPI-Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan., Hazawa M; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Division of Transdisciplinary Sciences, Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; WPI-Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan., Wong RW; School of Natural System, College of Science and Engineering, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Cell-Bionomics Research Unit, Institute for Frontier Science Initiative (INFINITI), Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; Division of Transdisciplinary Sciences, Graduate School of Frontier Science Initiative, Kanazawa University, Kanazawa, Ishikawa, 920-1192, Japan; WPI-Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192, Japan. Electronic address: rwong@staff.kanazawa-u.ac.jp.
المصدر: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Jan 15; Vol. 536, pp. 59-66. Date of Electronic Publication: 2020 Dec 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
أسماء مطبوعة: Publication: <2002- >: San Diego, CA : Elsevier
Original Publication: New York, Academic Press.
مواضيع طبية MeSH: Cell Nucleus Size*, Nuclear Matrix-Associated Proteins/*metabolism , Nuclear Pore Complex Proteins/*metabolism , Nucleocytoplasmic Transport Proteins/*metabolism , Viral Proteins/*metabolism, Active Transport, Cell Nucleus ; Cell Nucleus/virology ; HEK293 Cells ; Heterogeneous Nuclear Ribonucleoprotein A1/metabolism ; Humans ; SARS-CoV-2
مستخلص: The novel human betacoronavirus SARS-CoV-2 has caused an unprecedented pandemic in the 21st century. Several studies have revealed interactions between SARS-CoV-2 viral proteins and host nucleoporins, yet their functions are largely unknown. Here, we demonstrate that the open-reading frame 6 (ORF6) of SARS-CoV-2 can directly manipulate localization and functions of nucleoporins. We found that ORF6 protein disrupted nuclear rim staining of nucleoporins RAE1 and NUP98. Consequently, this disruption caused aberrant nucleocytoplasmic trafficking and led to nuclear accumulation of mRNA transporters such as hnRNPA1. Ultimately, host cell nucleus size was reduced and cell growth was halted.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:
(Copyright © 2020 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: NUP98; RAE1; SARS-CoV-2 ORF6; hnRNPA1
المشرفين على المادة: 0 (Heterogeneous Nuclear Ribonucleoprotein A1)
0 (Nuclear Matrix-Associated Proteins)
0 (Nuclear Pore Complex Proteins)
0 (Nucleocytoplasmic Transport Proteins)
0 (ORF6 protein, SARS-CoV-2)
0 (RAE1 protein, human)
0 (Viral Proteins)
0 (hnRNPA1 protein, human)
0 (nuclear pore complex protein 98)
تواريخ الأحداث: Date Created: 20201228 Date Completed: 20210115 Latest Revision: 20210115
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7733692
DOI: 10.1016/j.bbrc.2020.11.115
PMID: 33360543
قاعدة البيانات: MEDLINE
الوصف
تدمد:1090-2104
DOI:10.1016/j.bbrc.2020.11.115