دورية أكاديمية
Afucosylated IgG characterizes enveloped viral responses and correlates with COVID-19 severity.
| العنوان: | Afucosylated IgG characterizes enveloped viral responses and correlates with COVID-19 severity. |
|---|---|
| المؤلفون: | Larsen MD; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., de Graaf EL; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Sonneveld ME; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Plomp HR; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands., Nouta J; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands., Hoepel W; Department of Rheumatology and Clinical Immunology, Amsterdam UMC, Amsterdam Rheumatology and Immunology Center, Amsterdam, Netherlands.; Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Chen HJ; Department of Medical Biochemistry, Experimental Vascular Biology, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.; Department of Cardiovascular Sciences, Amsterdam Infection and Immunity Institute, University of Amsterdam, Amsterdam, Netherlands., Linty F; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Visser R; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Brinkhaus M; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Šuštić T; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., de Taeye SW; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Bentlage AEH; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Toivonen S; Finnish Red Cross Blood Service, Helsinki, Finland., Koeleman CAM; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands., Sainio S; Finnish Red Cross Blood Service, Helsinki, Finland., Kootstra NA; Department of Medical Microbiology, Amsterdam UMC, Amsterdam Infection and Immunity Institute, University of Amsterdam, Amsterdam, Netherlands., Brouwer PJM; Department of Medical Microbiology, Amsterdam UMC, Amsterdam Infection and Immunity Institute, University of Amsterdam, Amsterdam, Netherlands., Geyer CE; Department of Rheumatology and Clinical Immunology, Amsterdam UMC, Amsterdam Rheumatology and Immunology Center, Amsterdam, Netherlands.; Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Derksen NIL; Department of Immunopathology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Wolbink G; Amsterdam Rheumatology and Immunology Center, Reade, Amsterdam, Netherlands., de Winther M; Department of Medical Biochemistry, Experimental Vascular Biology, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.; Department of Cardiovascular Sciences, Amsterdam Infection and Immunity Institute, University of Amsterdam, Amsterdam, Netherlands., Sanders RW; Department of Medical Microbiology, Amsterdam UMC, Amsterdam Infection and Immunity Institute, University of Amsterdam, Amsterdam, Netherlands.; Weill Medical College, Cornell University, New York, USA., van Gils MJ; Department of Medical Microbiology, Amsterdam UMC, Amsterdam Infection and Immunity Institute, University of Amsterdam, Amsterdam, Netherlands., de Bruin S; Department of Intensive Care Medicine, Amsterdam UMC (Location AMC), University of Amsterdam, Amsterdam, Netherlands., Vlaar APJ; Department of Intensive Care Medicine, Amsterdam UMC (Location AMC), University of Amsterdam, Amsterdam, Netherlands., Rispens T; Department of Immunopathology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., den Dunnen J; Department of Rheumatology and Clinical Immunology, Amsterdam UMC, Amsterdam Rheumatology and Immunology Center, Amsterdam, Netherlands.; Department of Experimental Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Zaaijer HL; Department of Blood-borne Infections, Sanquin, Amsterdam, Netherlands., Wuhrer M; Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, Netherlands., Ellen van der Schoot C; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands., Vidarsson G; Department of Experimental Immunohematology, Sanquin Research, Amsterdam, Netherlands. g.vidarsson@sanquin.nl.; Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands. |
| مؤلفون مشاركون: | Amsterdam UMC COVID-19, biobank study group |
| المصدر: | Science (New York, N.Y.) [Science] 2021 Feb 26; Vol. 371 (6532). Date of Electronic Publication: 2020 Dec 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: New York, N.Y. : [s.n.] 1880- |
| مواضيع طبية MeSH: | Antibodies, Viral/*immunology , COVID-19/*immunology , Immunoglobulin G/*immunology , SARS-CoV-2/*immunology, Adult ; Aged ; Antibodies, Viral/blood ; Antibodies, Viral/chemistry ; COVID-19/physiopathology ; Cells, Cultured ; Critical Illness ; Cytomegalovirus/immunology ; Female ; Fucose/analysis ; Glycosylation ; HIV/immunology ; Hepatitis B Vaccines/immunology ; Humans ; Immunoglobulin Fc Fragments/chemistry ; Immunoglobulin Fc Fragments/immunology ; Immunoglobulin G/blood ; Immunoglobulin G/chemistry ; Inflammation ; Interleukin-6/biosynthesis ; Interleukin-6/immunology ; Macrophages/immunology ; Male ; Middle Aged ; Parvovirus B19, Human/immunology ; Severity of Illness Index ; Spike Glycoprotein, Coronavirus/immunology ; Vaccines, Subunit/immunology ; Young Adult |
| مستخلص: | Immunoglobulin G (IgG) antibodies are crucial for protection against invading pathogens. A highly conserved N-linked glycan within the IgG-Fc tail, which is essential for IgG function, shows variable composition in humans. Afucosylated IgG variants are already used in anticancer therapeutic antibodies for their increased activity through Fc receptors (FcγRIIIa). Here, we report that afucosylated IgG (approximately 6% of total IgG in humans) are specifically formed against enveloped viruses but generally not against other antigens. This mediates stronger FcγRIIIa responses but also amplifies brewing cytokine storms and immune-mediated pathologies. Critically ill COVID-19 patients, but not those with mild symptoms, had high concentrations of afucosylated IgG antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), amplifying proinflammatory cytokine release and acute phase responses. Thus, antibody glycosylation plays a critical role in immune responses to enveloped viruses, including COVID-19. (Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.) |
| References: | Lancet. 1987 Oct 17;2(8564):921. (PMID: 2889120) Science. 2002 Dec 13;298(5601):2199-202. (PMID: 12481138) Blood. 2014 Jan 23;123(4):471-80. (PMID: 24243971) J Immunol. 2019 Dec 15;203(12):3126-3135. (PMID: 31748349) J Infect. 2020 Jun;80(6):607-613. (PMID: 32283152) Transfusion. 2015 Mar;55(3):553-62. (PMID: 25234110) J Proteome Res. 2013 Feb 1;12(2):821-31. (PMID: 23298168) Proc Natl Acad Sci U S A. 2011 Aug 2;108(31):12669-74. (PMID: 21768335) Proteomics. 2007 Nov;7(22):4070-81. (PMID: 17994628) J Med Virol. 2020 May;92(5):522-528. (PMID: 32027036) Br J Haematol. 2014 Sep;166(6):936-45. (PMID: 24909983) Br J Haematol. 2016 Jul;174(2):310-20. (PMID: 27017954) Sci Rep. 2016 Nov 22;6:36964. (PMID: 27872474) JCI Insight. 2019 Feb 21;4(4):. (PMID: 30830861) Br J Haematol. 2017 Feb;176(4):651-660. (PMID: 27891581) Front Immunol. 2014 Oct 20;5:520. (PMID: 25368619) Front Immunol. 2017 Aug 02;8:877. (PMID: 28824618) J Proteome Res. 2016 Jun 3;15(6):1853-61. (PMID: 27161864) Lupus. 2016 Jul;25(8):934-42. (PMID: 27252272) J Proteome Res. 2009 Feb;8(2):450-6. (PMID: 18942870) Mol Cell Proteomics. 2012 Apr;11(4):M111.014563. (PMID: 22184099) J Immunol. 2020 Dec 15;205(12):3491-3499. (PMID: 33127820) Lancet. 2020 Feb 15;395(10223):497-506. (PMID: 31986264) Front Immunol. 2018 Mar 19;9:553. (PMID: 29616041) Adv Virus Res. 2017;97:187-243. (PMID: 28057259) J Clin Invest. 2013 May;123(5):2183-92. (PMID: 23563315) Medicine (Baltimore). 2016 Jul;95(28):e4112. (PMID: 27428197) Methods Mol Biol. 2017;1503:31-47. (PMID: 27743357) Front Immunol. 2019 Dec 11;10:2887. (PMID: 31921150) J Biol Chem. 2002 Jul 26;277(30):26733-40. (PMID: 11986321) Science. 2020 Aug 7;369(6504):643-650. (PMID: 32540902) MAbs. 2016;8(2):197-204. (PMID: 26651519) Cell. 2015 Jul 2;162(1):160-9. (PMID: 26140596) J Gerontol A Biol Sci Med Sci. 2014 Jul;69(7):779-89. (PMID: 24325898) Intensive Care Med. 2020 Apr;46(4):579-582. (PMID: 32103284) Nat Rev Drug Discov. 2009 Mar;8(3):226-34. (PMID: 19247305) Science. 2017 Jan 27;355(6323):395-398. (PMID: 28126818) |
| فهرسة مساهمة: | Investigator: M van Agtmael; AG Algera; F van Baarle; D Bax; D van de Beek; M Beudel; HJ Bogaard; PI Bonta; M Bomers; L Bos; M Botta; G de Bree; MC Brouwer; J Brabander; S de Bruin; M Bugiani; E Bulle; O Chouchane; A Cloherty; P Elbers; L Fleuren; S Geerlings; B Geerts; T Geijtenbeek; A Girbes; B Goorhuis; MP Grobusch; F Hafkamp; L Hagens; J Hamann; V Harris; R Hemke; SM Hermans; L Heunks; MW Hollmann; J Horn; JW Hovius; M de Jong; R Koning; N van Mourik; J Nellen; EJ Nossent; F Paulus; E Peters; T van der Poll; B Preckel; JM Prins; J Raasveld; T Rijnders; M Schinkel; M Schultz; AR Schuurmans; K Sigaloff; M Smit; CS Stijnis; W Stilma; C Teunissen; P Thoral; A Tsonas; M van der Valk; D Veelo; APJ Vlaar; H de Vries; M van Vugt; WJ Wiersinga; D Wouters; AHK Zwinderman |
| المشرفين على المادة: | 0 (Antibodies, Viral) 0 (Hepatitis B Vaccines) 0 (IL6 protein, human) 0 (Immunoglobulin Fc Fragments) 0 (Immunoglobulin G) 0 (Interleukin-6) 0 (Spike Glycoprotein, Coronavirus) 0 (Vaccines, Subunit) 0 (spike protein, SARS-CoV-2) 28RYY2IV3F (Fucose) |
| تواريخ الأحداث: | Date Created: 20201228 Date Completed: 20210305 Latest Revision: 20210521 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7919849 |
| DOI: | 10.1126/science.abc8378 |
| PMID: | 33361116 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9203 |
|---|---|
| DOI: | 10.1126/science.abc8378 |