دورية أكاديمية
أعرض في EDS

Natterin an aerolysin-like fish toxin drives IL-1β-dependent neutrophilic inflammation mediated by caspase-1 and caspase-11 activated by the inflammasome sensor NLRP6.

التفاصيل البيبلوغرافية
العنوان: Natterin an aerolysin-like fish toxin drives IL-1β-dependent neutrophilic inflammation mediated by caspase-1 and caspase-11 activated by the inflammasome sensor NLRP6.
المؤلفون: Lima C; Immunoregulation Unit of the Laboratory of Applied Toxinology (CETICs/FAPESP), Butantan Institute, Vital Brazil Avenue, 1500. Butantan, 05503-009 São Paulo. Brazil. Electronic address: carla.lima@butantan.gov.br., Falcao MAP; Immunoregulation Unit of the Laboratory of Applied Toxinology (CETICs/FAPESP), Butantan Institute, Vital Brazil Avenue, 1500. Butantan, 05503-009 São Paulo. Brazil., Andrade-Barros AI; Immunoregulation Unit of the Laboratory of Applied Toxinology (CETICs/FAPESP), Butantan Institute, Vital Brazil Avenue, 1500. Butantan, 05503-009 São Paulo. Brazil., Seni-Silva AC; Immunoregulation Unit of the Laboratory of Applied Toxinology (CETICs/FAPESP), Butantan Institute, Vital Brazil Avenue, 1500. Butantan, 05503-009 São Paulo. Brazil., Grund LZ; Immunoregulation Unit of the Laboratory of Applied Toxinology (CETICs/FAPESP), Butantan Institute, Vital Brazil Avenue, 1500. Butantan, 05503-009 São Paulo. Brazil., Balogh E; Research Centre for Molecular Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei Krt 98, 4012 Debrecen, Hungary., Conceiçao K; Peptide Biochemistry Laboratory, UNIFESP, São José dos Campos. Brazil., Queniaux VF; Allergy and Lung Inflammation Unit of the Molecular and Experimental Immunology and Neurogenetics (INEM, UMR7355, CNRS and University Orléans), Orléans, 45071 Orléans Cedex 2, France., Ryffel B; Allergy and Lung Inflammation Unit of the Molecular and Experimental Immunology and Neurogenetics (INEM, UMR7355, CNRS and University Orléans), Orléans, 45071 Orléans Cedex 2, France., Lopes-Ferreira M; Immunoregulation Unit of the Laboratory of Applied Toxinology (CETICs/FAPESP), Butantan Institute, Vital Brazil Avenue, 1500. Butantan, 05503-009 São Paulo. Brazil.
المصدر: International immunopharmacology [Int Immunopharmacol] 2021 Feb; Vol. 91, pp. 107287. Date of Electronic Publication: 2020 Dec 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam ; New York : Elsevier Science, c2001-
مواضيع طبية MeSH: Caspase 1/*metabolism , Caspases, Initiator/*metabolism , Fish Venoms/*pharmacology , Inflammasomes/*metabolism , Inflammation/*immunology , Interleukin-1beta/*metabolism , Lung/*drug effects , Neutrophil Infiltration/*drug effects , Neutrophils/*drug effects , Peritonitis/*chemically induced , Receptors, Cell Surface/*metabolism, Animals ; Apoptosis Regulatory Proteins/genetics ; Apoptosis Regulatory Proteins/metabolism ; CARD Signaling Adaptor Proteins/genetics ; CARD Signaling Adaptor Proteins/metabolism ; Calcium-Binding Proteins/genetics ; Calcium-Binding Proteins/metabolism ; Caspase 1/genetics ; Caspases, Initiator/genetics ; Female ; Inflammasomes/immunology ; Inflammation Mediators/metabolism ; Interleukin-1beta/genetics ; Intracellular Signaling Peptides and Proteins/genetics ; Intracellular Signaling Peptides and Proteins/metabolism ; Lung/enzymology ; Lung/immunology ; Male ; Mice, Inbred C57BL ; Mice, Knockout ; Neutrophils/enzymology ; Neutrophils/immunology ; Peritonitis/enzymology ; Peritonitis/genetics ; Peritonitis/immunology ; Phosphate-Binding Proteins/genetics ; Phosphate-Binding Proteins/metabolism ; Pore Forming Cytotoxic Proteins ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/immunology ; Signal Transduction ; Mice
مستخلص: Natterin is an aerolysin-like pore-forming toxin responsible for the toxic effects of the venom of the medically significant fish Thalassophryne nattereri. Using a combination of pharmacologic and genetic loss-of-function approaches we conduct a systematic investigation of the regulatory mechanisms that control Natterin-induced neutrophilic inflammation in the peritonitis model. Our data confirmed the capacity of Natterin to induce a strong and sustained neutrophilic inflammation leading to systemic inflammatory lung infiltration and revealed overlapping regulatory paths in its control. We found that Natterin induced the extracellular release of mature IL-1β and the sustained production of IL-33 by bronchial epithelial cells. We confirmed the dependence of both ST2/IL-33 and IL-17A/IL-17RA signaling on the local and systemic neutrophils migration, as well as the crucial role of IL-1α, caspase-1 and caspase-11 for neutrophilic inflammation. The inflammation triggered by Natterin was a gasdermin-D-dependent inflammasome process, despite the cells did not die by pyroptosis. Finally, neutrophilic inflammation was mediated by non-canonical NLRP6 and NLRC4 adaptors through ASC interaction, independent of NLRP3. Our data highlight that the inflammatory process dependent on non-canonical inflammasome activation can be a target for pharmacological intervention in accidents by T. nattereri, which does not have adequate specific therapy.
(Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Aerolysin; Caspase-11/caspase-1; IL-1α/β; NLPR6/NLRC4; Natterin; Neutrophilia
المشرفين على المادة: 0 (Apoptosis Regulatory Proteins)
0 (CARD Signaling Adaptor Proteins)
0 (Calcium-Binding Proteins)
0 (Fish Venoms)
0 (Gsdmd protein, mouse)
0 (IL1B protein, mouse)
0 (Inflammasomes)
0 (Inflammation Mediators)
0 (Interleukin-1beta)
0 (Intracellular Signaling Peptides and Proteins)
0 (Ipaf protein, mouse)
0 (Nod-like receptor pyrin domain-containing protein 6, mouse)
0 (Phosphate-Binding Proteins)
0 (Pore Forming Cytotoxic Proteins)
0 (Pycard protein, mouse)
0 (Receptors, Cell Surface)
0 (natterin)
EC 3.4.22.- (Casp4 protein, mouse)
EC 3.4.22.- (Caspases, Initiator)
EC 3.4.22.36 (Casp1 protein, mouse)
EC 3.4.22.36 (Caspase 1)
تواريخ الأحداث: Date Created: 20201230 Date Completed: 20210526 Latest Revision: 20240226
رمز التحديث: 20240226
DOI: 10.1016/j.intimp.2020.107287
PMID: 33378723
قاعدة البيانات: MEDLINE
الوصف
تدمد:1878-1705
DOI:10.1016/j.intimp.2020.107287