دورية أكاديمية
أعرض في EDS

Effect of Quercetin Supplementation in Extender on Sperm Kinematics, Extracellular Enzymes Release, and Oxidative Stress of Egyptian Buffalo Bulls Frozen-Thawed Semen.

التفاصيل البيبلوغرافية
العنوان: Effect of Quercetin Supplementation in Extender on Sperm Kinematics, Extracellular Enzymes Release, and Oxidative Stress of Egyptian Buffalo Bulls Frozen-Thawed Semen.
المؤلفون: El-Khawagah ARM; Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt., Kandiel MMM; Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Toukh, Egypt., Samir H; Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
المصدر: Frontiers in veterinary science [Front Vet Sci] 2020 Dec 14; Vol. 7, pp. 604460. Date of Electronic Publication: 2020 Dec 14 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2014]-
مستخلص: Buffalo spermatozoa are more sensitive for cryopreservation compared to other species. This study aimed to evaluate the consequences of quercetin against cryodamage of buffalo frozen-thawed spermatozoa characteristics. Semen of Egyptian bulls ( n = 4) was extended in OptiXcell extender incorporated with quercetin at 0 (control), 2.5, 5.0, 10.0, 20.0, 40.0, and 80.0 μM before cryopreservation. Frozen-thawed semen was evaluated for sperm motility by computer-assisted sperm analyzer (CASA), viability, morphology, membrane, and acrosome integrities. The kinematics parameters including average path velocity (VAP; μm/s), straight linear velocity (VSL; μm/s), curvilinear velocity (VCL; μm/s), amplitude of lateral head displacement (ALH; μm), beat cross frequency (BCF; Hz), linearity [LIN, (VSL/VCL) × 100], and straightness [STR, (VSL/VAP) × 100] were assessed. The sperm-free extender was evaluated for aspartate aminotransferase (AST), alanine aminotransferase (ALT), and H 2 O 2 . Homogenized sperm cells were evaluated for oxidative stress biomarkers [superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX)], and lipid peroxidation [malondialdehyde (MDA)]. The highest values of total motility, progressive motility, viability, intact acrosome, and membrane integrity substantially improved with 10 μM of quercetin. STR (%) was substantially low ( P < 0.01), and VCL (μm/s) and ALH (μm) were markedly high ( P < 0.05) in 10 μM of quercetin. The outflow of ALT enzyme to extracellular fluid was lower with 10 μM of quercetin ( P < 0.001) and higher at 2.5 μM of quercetin. The spermatozoa leaked AST was markedly lower at 5.0, 10 ( P < 0.001) and 20 μM ( P < 0.05) of quercetin. The activity of antioxidant enzymes was eminently low at all quercetin concentrations, and this was accompanied by the decrease in H 2 O 2 in the media. SOD activity at 10-80 μM, CAT at 5.0-40 μM, and GPX at 2.5-80.0 μM of quercetin in spermatozoa were substantially low. MDA level significantly ( P < 0.001) decreased at all quercetin concentrations. In conclusion, the incorporation of quercetin at the level of 10 μM is promising in improving buffalo semen characteristics and lower the freezing-thawing oxidative stress.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2020 El-Khawagah, Kandiel and Samir.)
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فهرسة مساهمة: Keywords: CASA; Egyptian buffalo; frozen semen; oxidative stress markers; quercetin; sperm kinetics
تواريخ الأحداث: Date Created: 20201231 Latest Revision: 20210101
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7768016
DOI: 10.3389/fvets.2020.604460
PMID: 33381536
قاعدة البيانات: MEDLINE
الوصف
تدمد:2297-1769
DOI:10.3389/fvets.2020.604460