دورية أكاديمية
Adverse effect of superoxide-induced mitochondrial damage in granulosa cells on follicular development in mouse ovaries.
| العنوان: | Adverse effect of superoxide-induced mitochondrial damage in granulosa cells on follicular development in mouse ovaries. |
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| المؤلفون: | Hoque SAM; Laboratory of Reproductive Endocrinology, Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan; Department of Animal Breeding and Genetics, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh., Umehara T; Laboratory of Reproductive Biology, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan., Kawai T; Laboratory of Reproductive Biology, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan., Shimada M; Laboratory of Reproductive Endocrinology, Graduate School of Biosphere Science, Hiroshima University, Hiroshima, Japan; Laboratory of Reproductive Biology, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan. Electronic address: mashimad@hiroshima-u.ac.jp. |
| المصدر: | Free radical biology & medicine [Free Radic Biol Med] 2021 Feb 01; Vol. 163, pp. 344-355. Date of Electronic Publication: 2020 Dec 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 8709159 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4596 (Electronic) Linking ISSN: 08915849 NLM ISO Abbreviation: Free Radic Biol Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Tarrytown, NY : Elsevier Science Original Publication: New York : Pergamon, c1987- |
| مواضيع طبية MeSH: | Ovary* , Superoxides*, Animals ; Female ; Follicle Stimulating Hormone ; Granulosa Cells ; Mice ; Ovulation |
| مستخلص: | High mitochondrial oxidative phosphorylation (mt-OXPHOS) levels are required to supply the ATP necessary for follicle-stimulating hormone (FSH)-induced granulosa cell proliferation during the follicular development process. Consequently, excessive reactive oxygen species (ROS) might be generated and have an adverse effect on follicular health. This study aimed to elucidate the negative effects of ROS on mitochondrial functions in FSH-stimulated granulosa cells during the follicular development process and to investigate whether pyrroloquinoline quinone (PQQ) treatment could accelerate this process by ameliorating the adverse effects. To do this, both in vitro and in vivo experiments were performed with granulosa cells from superovulated immature (3-week-old) mice that were pretreated with or without PQQ, and a natural mating study was also performed. The ROS level in FSH-/eCG-stimulated granulosa cells was significantly increased. Moreover, high oxidative stress and mtDNA damage levels were evident in the granulosa cells. PQQ treatment not only reduced the ROS and oxidative stress levels but also ameliorated mtDNA damage, accelerated FSH-/eCG-induced ATP production, and increased the mitochondrial membrane potential and the expression levels of mitochondrial genes (Nd1, Cytb, Cox1, ATPase6) and the mt-ND1 protein. Accordingly, the proliferation and viability of granulosa cells, numbers of healthy preovulatory follicles and ovulated oocytes and serum estrogen level were significantly improved, while the apoptosis of granulosa cells was reduced. However, PQQ treatment did not change the fertility parameters in mature mice with natural cycles but did significantly increased the number of offspring born per delivery. These results revealed that ROS-associated damage in FSH-stimulated granulosa cells adversely affects their physiology and follicular health during the follicular development process. Treatment with PQQ is a beneficial tool to increase both the number of ovulated oocytes and pups per delivery. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Granulosa cells; Mitochondria; Ovarian follicles; Pyrroloquinoline quinone (PQQ); Reactive oxygen species (ROS) |
| المشرفين على المادة: | 11062-77-4 (Superoxides) 9002-68-0 (Follicle Stimulating Hormone) |
| تواريخ الأحداث: | Date Created: 20210101 Date Completed: 20210622 Latest Revision: 20210622 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.freeradbiomed.2020.12.434 |
| PMID: | 33385538 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-4596 |
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| DOI: | 10.1016/j.freeradbiomed.2020.12.434 |