دورية أكاديمية
Cell Sorting Using Electrokinetic Deterministic Lateral Displacement.
| العنوان: | Cell Sorting Using Electrokinetic Deterministic Lateral Displacement. |
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| المؤلفون: | Ho BD; Division of Solid State Physics and NanoLund, Physics Department, Lund University, PO Box 118, 22100 Lund, Sweden., Beech JP; Division of Solid State Physics and NanoLund, Physics Department, Lund University, PO Box 118, 22100 Lund, Sweden., Tegenfeldt JO; Division of Solid State Physics and NanoLund, Physics Department, Lund University, PO Box 118, 22100 Lund, Sweden. |
| المصدر: | Micromachines [Micromachines (Basel)] 2020 Dec 30; Vol. 12 (1). Date of Electronic Publication: 2020 Dec 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101640903 Publication Model: Electronic Cited Medium: Print ISSN: 2072-666X (Print) Linking ISSN: 2072666X NLM ISO Abbreviation: Micromachines (Basel) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2010]- |
| مستخلص: | We show that by combining deterministic lateral displacement (DLD) with electrokinetics, it is possible to sort cells based on differences in their membrane and/or internal structures. Using heat to deactivate cells, which change their viability and structure, we then demonstrate sorting of a mixture of viable and non-viable cells for two different cell types. For Escherichia coli, the size change due to deactivation is insufficient to allow size-based DLD separation. Our method instead leverages the considerable change in zeta potential to achieve separation at low frequency. Conversely, for Saccharomyces cerevisiae (Baker's yeast) the heat treatment does not result in any significant change of zeta potential. Instead, we perform the sorting at higher frequency and utilize what we believe is a change in dielectrophoretic mobility for the separation. We expect our work to form a basis for the development of simple, low-cost, continuous label-free methods that can separate cells and bioparticles based on their intrinsic properties. |
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| معلومات مُعتمدة: | 607350 Seventh Framework Programme; 801367 Horizon 2020 Framework Programme; 634890 Horizon 2020 Framework Programme; 2016-05739 Vetenskapsrådet |
| فهرسة مساهمة: | Keywords: charge-based separation; dielectrophoresis; electrokinetic deterministic lateral displacement |
| تواريخ الأحداث: | Date Created: 20210105 Latest Revision: 20210218 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7823954 |
| DOI: | 10.3390/mi12010030 |
| PMID: | 33396630 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2072-666X |
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| DOI: | 10.3390/mi12010030 |