دورية أكاديمية
أعرض في EDS

Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray.

التفاصيل البيبلوغرافية
العنوان: Analysis of SARS-CoV-2 antibodies in COVID-19 convalescent blood using a coronavirus antigen microarray.
المؤلفون: de Assis RR; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Jain A; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Nakajima R; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Jasinskas A; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Felgner J; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Obiero JM; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Norris PJ; Vitalant Research Institute, San Francisco, CA, USA.; Department of Laboratory Medicine, University of California, San Francisco, CA, USA., Stone M; Vitalant Research Institute, San Francisco, CA, USA.; Department of Laboratory Medicine, University of California, San Francisco, CA, USA., Simmons G; Vitalant Research Institute, San Francisco, CA, USA.; Department of Laboratory Medicine, University of California, San Francisco, CA, USA., Bagri A; Cerus Corporation, Concord, CA, USA., Irsch J; Cerus Corporation, Concord, CA, USA., Schreiber M; Department of Surgery, Oregon Health & Science University, Portland, OR, USA., Buser A; Division of Infectious Diseases & Hospital Epidemiology, University Hospital Basel, University of Basel, Basel, Switzerland., Holbro A; Department of Surgery, Oregon Health & Science University, Portland, OR, USA., Battegay M; Division of Infectious Diseases & Hospital Epidemiology, University Hospital Basel, University of Basel, Basel, Switzerland., Hosimer P; Ortho Clinical Diagnostics, Rochester, NY, USA., Noesen C; Ortho Clinical Diagnostics, Rochester, NY, USA., Adenaiye O; Institute for Applied Environmental Health, School of Public Health, University of Maryland, College Park, MD, USA., Tai S; Institute for Applied Environmental Health, School of Public Health, University of Maryland, College Park, MD, USA., Hong F; Institute for Applied Environmental Health, School of Public Health, University of Maryland, College Park, MD, USA., Milton DK; Institute for Applied Environmental Health, School of Public Health, University of Maryland, College Park, MD, USA., Davies DH; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Contestable P; Ortho Clinical Diagnostics, Rochester, NY, USA., Corash LM; Cerus Corporation, Concord, CA, USA., Busch MP; Vitalant Research Institute, San Francisco, CA, USA.; Department of Laboratory Medicine, University of California, San Francisco, CA, USA., Felgner PL; Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA, USA., Khan S; Division of Infectious Diseases, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. saahirkh@usc.edu.
المصدر: Nature communications [Nat Commun] 2021 Jan 04; Vol. 12 (1), pp. 6. Date of Electronic Publication: 2021 Jan 04.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Antibodies, Viral/*blood , Antigens, Viral/*blood , COVID-19/*immunology , SARS-CoV-2/*immunology, Antibodies, Viral/immunology ; Antigens, Viral/immunology ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19 Testing ; Humans ; Immunoglobulin G/blood ; Immunoglobulin G/immunology ; Immunoglobulin M/blood ; Immunoglobulin M/immunology ; Microarray Analysis/methods ; Middle East Respiratory Syndrome Coronavirus/immunology ; Neutralization Tests ; Severe acute respiratory syndrome-related coronavirus/immunology ; Spike Glycoprotein, Coronavirus/immunology
مستخلص: The current practice for diagnosis of COVID-19, based on SARS-CoV-2 PCR testing of pharyngeal or respiratory specimens in a symptomatic patient at high epidemiologic risk, likely underestimates the true prevalence of infection. Serologic methods can more accurately estimate the disease burden by detecting infections missed by the limited testing performed to date. Here, we describe the validation of a coronavirus antigen microarray containing immunologically significant antigens from SARS-CoV-2, in addition to SARS-CoV, MERS-CoV, common human coronavirus strains, and other common respiratory viruses. A comparison of antibody profiles detected on the array from control sera collected prior to the SARS-CoV-2 pandemic versus convalescent blood specimens from virologically confirmed COVID-19 cases demonstrates near complete discrimination of these two groups, with improved performance from use of antigen combinations that include both spike protein and nucleoprotein. This array can be used as a diagnostic tool, as an epidemiologic tool to more accurately estimate the disease burden of COVID-19, and as a research tool to correlate antibody responses with clinical outcomes.
التعليقات: Update of: bioRxiv. 2020 May 08;:. (PMID: 32511302)
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معلومات مُعتمدة: KL2 TR001416 United States TR NCATS NIH HHS
المشرفين على المادة: 0 (Antibodies, Viral)
0 (Antigens, Viral)
0 (Immunoglobulin G)
0 (Immunoglobulin M)
0 (Spike Glycoprotein, Coronavirus)
تواريخ الأحداث: Date Created: 20210105 Date Completed: 20210113 Latest Revision: 20221207
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7782488
DOI: 10.1038/s41467-020-20095-2
PMID: 33397903
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-020-20095-2