دورية أكاديمية
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Functional Comparison between VP64-dCas9-VP64 and dCas9-VP192 CRISPR Activators in Human Embryonic Kidney Cells.

التفاصيل البيبلوغرافية
العنوان: Functional Comparison between VP64-dCas9-VP64 and dCas9-VP192 CRISPR Activators in Human Embryonic Kidney Cells.
المؤلفون: Javaid N; Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea., Pham TLH; Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea., Choi S; Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea.; S&K Therapeutics, Woncheon Hall 135, Ajou University, Suwon 16499, Korea.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2021 Jan 01; Vol. 22 (1). Date of Electronic Publication: 2021 Jan 01.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: CRISPR-Associated Protein 9* , Transcriptional Activation*, Gene Editing/*methods , Octamer Transcription Factor-3/*genetics , SOXB1 Transcription Factors/*genetics, Gene Expression Regulation ; HEK293 Cells ; Humans ; RNA, Messenger ; Up-Regulation
مستخلص: Reversal in the transcriptional status of desired genes has been exploited for multiple research, therapeutic, and biotechnological purposes. CRISPR/dCas9-based activators can activate transcriptionally silenced genes after being guided by gene-specific gRNA(s). Here, we performed a functional comparison between two such activators, VP64-dCas9-VP64 and dCas9-VP192, in human embryonic kidney cells by the concomitant targeting of POU5F1 and SOX2 . We found 22- and 6-fold upregulations in the mRNA level of POU5F1 by dCas9-VP192 and VP64-dCas9-VP64, respectively. Likewise, SOX2 was up-regulated 4- and 2-fold using dCas9-VP192 and VP64dCas9VP64, respectively. For the POU5F1 protein level, we observed 3.7- and 2.2-fold increases with dCas9-VP192 and VP64-dCas9-VP64, respectively. Similarly, the SOX2 expression was 2.4- and 2-fold higher with dCas9-VP192 and VP64-dCas9-VP64, respectively. We also confirmed that activation only happened upon co-transfecting an activator plasmid with multiplex gRNA plasmid with a high specificity to the reference genes. Our data revealed that dCas9-VP192 is more efficient than VP64-dCas9-VP64 for activating reference genes.
References: Nat Commun. 2014 Jun 26;5:4240. (PMID: 24967838)
Science. 2003 Aug 8;301(5634):798-802. (PMID: 12907790)
Anal Bioanal Chem. 2010 Aug;397(8):3173-8. (PMID: 20549496)
Plant Cell. 2014 Jan;26(1):151-63. (PMID: 24443519)
Science. 2009 Dec 11;326(5959):1509-12. (PMID: 19933107)
Nature. 2007 Jul 19;448(7151):313-7. (PMID: 17554338)
Nat Commun. 2016 Aug 30;7:12642. (PMID: 27572891)
Cell. 2007 Feb 23;128(4):693-705. (PMID: 17320507)
Plant Biotechnol J. 2015 May;13(4):578-89. (PMID: 25400128)
Nat Methods. 2013 Oct;10(10):977-9. (PMID: 23892898)
Cell Stem Cell. 2008 Jan 10;2(1):10-2. (PMID: 18371415)
Science. 2005 Aug 26;309(5739):1369-73. (PMID: 16123299)
Cell Mol Life Sci. 2016 Nov;73(22):4315-4325. (PMID: 27178736)
Sci Rep. 2014 Jun 23;4:5400. (PMID: 24954249)
Nat Methods. 2013 Oct;10(10):973-6. (PMID: 23892895)
Cell. 1994 May 20;77(4):599-608. (PMID: 8187178)
Nat Methods. 2015 Apr;12(4):326-8. (PMID: 25730490)
Genes Dev. 2010 Oct 15;24(20):2239-63. (PMID: 20952534)
EMBO J. 2002 Mar 1;21(5):1121-31. (PMID: 11867540)
Nature. 2014 Oct 16;514(7522):380-4. (PMID: 25119044)
Nat Med. 2002 Dec;8(12):1427-32. (PMID: 12415262)
Cell Reprogram. 2017 Jun;19(3):189-198. (PMID: 28557624)
Nat Methods. 2012 Feb 19;9(4):363-6. (PMID: 22343343)
Nat Biotechnol. 2003 Mar;21(3):269-74. (PMID: 12592412)
Biomed Res Int. 2015;2015:326042. (PMID: 26697483)
Nat Plants. 2017 Dec;3(12):930-936. (PMID: 29158545)
Nature. 2015 Jan 29;517(7536):583-8. (PMID: 25494202)
Front Cell Dev Biol. 2015 Feb 02;3:2. (PMID: 25699255)
Cell Res. 2011 Jan;21(1):196-204. (PMID: 20956998)
Mol Pharmacol. 2004 Dec;66(6):1361-71. (PMID: 15340042)
Science. 2014 Jan 3;343(6166):84-87. (PMID: 24336571)
Nature. 2008 Jan 10;451(7175):141-6. (PMID: 18157115)
Nature. 2015 Nov 5;527(7576):110-3. (PMID: 26524520)
Nat Methods. 2013 Mar;10(3):243-5. (PMID: 23396285)
Cell Stem Cell. 2018 Feb 1;22(2):252-261.e4. (PMID: 29358044)
Cell. 2006 Aug 25;126(4):663-76. (PMID: 16904174)
Nat Biotechnol. 2015 May;33(5):510-7. (PMID: 25849900)
Nucleic Acids Res. 2012 Jul;40(12):5368-77. (PMID: 22387464)
Cell. 2015 Jan 15;160(1-2):339-50. (PMID: 25533786)
Science. 2001 Apr 6;292(5514):110-3. (PMID: 11283354)
Adv Drug Deliv Rev. 2009 Jul 2;61(7-8):513-26. (PMID: 19394375)
Nat Biotechnol. 2014 Apr;32(4):347-55. (PMID: 24584096)
Nat Biotechnol. 2014 Mar;32(3):267-73. (PMID: 24535568)
Nature. 2007 Nov 22;450(7169):497-502. (PMID: 18004281)
Nucleic Acids Res. 2013 Aug;41(15):7429-37. (PMID: 23761437)
Cell. 2013 Dec 19;155(7):1479-91. (PMID: 24360272)
Cell. 2016 Sep 22;167(1):233-247.e17. (PMID: 27662091)
Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1495-500. (PMID: 10660690)
Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):E2579-86. (PMID: 22949671)
Nat Methods. 2016 Jul;13(7):563-567. (PMID: 27214048)
Nat Commun. 2017 Nov 9;8(1):1375. (PMID: 29123204)
Cell Stem Cell. 2007 Jun 7;1(1):55-70. (PMID: 18371336)
Nat Biotechnol. 2008 Jan;26(1):101-6. (PMID: 18059259)
Nat Commun. 2014 Aug 14;5:4657. (PMID: 25118589)
Nature. 2007 Jul 19;448(7151):318-24. (PMID: 17554336)
Nat Biotechnol. 2014 Aug;32(8):819-21. (PMID: 24880488)
Nat Biotechnol. 2003 Jun;21(6):635-7. (PMID: 12754523)
Nat Protoc. 2013 Nov;8(11):2180-96. (PMID: 24136345)
Nature. 2012 Sep 6;489(7414):101-8. (PMID: 22955620)
Trends Biotechnol. 2013 Jul;31(7):397-405. (PMID: 23664777)
Virus Res. 2018 Jan 15;244:296-303. (PMID: 28456574)
Nat Methods. 2013 Mar;10(3):239-42. (PMID: 23377379)
Nature. 2016 Nov 17;539(7629):384-389. (PMID: 27820943)
In Vitro Cell Dev Biol Anim. 1995 May;31(5):347-51. (PMID: 7633672)
Mol Ther Nucleic Acids. 2014 Dec 16;3:e216. (PMID: 25514649)
Nat Biotechnol. 2003 Oct;21(10):1208-14. (PMID: 12960965)
Methods Mol Biol. 2000;130:147-53. (PMID: 10589428)
Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):2883-8. (PMID: 18287077)
Annu Rev Biochem. 2016 Jun 2;85:227-64. (PMID: 27145843)
Nat Biotechnol. 2008 Nov;26(11):1269-75. (PMID: 18849973)
Cell Stem Cell. 2016 Sep 1;19(3):406-14. (PMID: 27524438)
Nat Commun. 2015 Mar 10;6:6413. (PMID: 25752527)
Science. 2009 Dec 11;326(5959):1501. (PMID: 19933106)
Nucleic Acids Res. 2014 Oct 29;42(19):e147. (PMID: 25122746)
Cell Rep. 2015 Apr 7;11(1):51-60. (PMID: 25818303)
Nat Methods. 2014 Sep;11(9):915-8. (PMID: 25108687)
Cell Res. 2013 Oct;23(10):1163-71. (PMID: 23979020)
Oncogene. 2007 Apr 26;26(19):2791-8. (PMID: 17057734)
Genes (Basel). 2017 Aug 07;8(8):. (PMID: 28783137)
Bone. 2014 Dec;69:118-25. (PMID: 25260929)
Curr Opin Neurobiol. 2002 Oct;12(5):566-73. (PMID: 12367637)
Nat Biotechnol. 2011 Feb;29(2):149-53. (PMID: 21248753)
Curr Biol. 2001 Oct 2;11(19):1553-8. (PMID: 11591326)
Oncotarget. 2017 Apr 18;8(16):26027-26040. (PMID: 28212528)
J Biomol Tech. 2010 Jul;21(2):66-72. (PMID: 20592869)
Biochem Cell Biol. 2005 Jun;83(3):270-85. (PMID: 15959555)
Plant Biotechnol J. 2017 Feb;15(2):257-268. (PMID: 27510362)
Cell Stem Cell. 2010 Dec 3;7(6):651-5. (PMID: 21112560)
Stem Cells. 2003;21(6):632-7. (PMID: 14595122)
Nature. 1997 Feb 27;385(6619):810-3. (PMID: 9039911)
Sci Adv. 2017 Aug 04;3(8):eaao0027. (PMID: 28808686)
Nat Cell Biol. 2012 Feb 19;14(3):318-28. (PMID: 22344029)
Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):E2739-E2747. (PMID: 28292896)
PLoS Genet. 2013;9(9):e1003742. (PMID: 24039596)
Nat Biotechnol. 2016 May;34(5):528-30. (PMID: 27088723)
Cell. 2013 Feb 28;152(5):1173-83. (PMID: 23452860)
Science. 2014 Mar 14;343(6176):1247997. (PMID: 24505130)
Plant Biotechnol J. 2018 Nov;16(11):1918-1927. (PMID: 29604159)
Nat Rev Genet. 2012 Sep;13(9):613-26. (PMID: 22868264)
Plant Biotechnol J. 2019 May;17(5):858-868. (PMID: 30291759)
Cell Rep. 2015 Sep 8;12(10):1668-77. (PMID: 26321643)
Cell Stem Cell. 2013 Dec 5;13(6):659-62. (PMID: 24315440)
Stem Cell Reports. 2014 Dec 9;3(6):940-7. (PMID: 25448066)
Plant Biotechnol J. 2019 Feb;17(2):362-372. (PMID: 29972722)
Nature. 1958 Jul 5;182(4627):64-5. (PMID: 13566187)
Science. 2014 Jan 3;343(6166):80-4. (PMID: 24336569)
Stem Cells. 2018 Dec;36(12):1851-1862. (PMID: 30270540)
Sci Rep. 2016 Jan 19;6:19648. (PMID: 26782778)
Nature. 2014 Dec 18;516(7531):423-7. (PMID: 25337876)
Cell. 2007 Nov 30;131(5):861-72. (PMID: 18035408)
Science. 2012 Aug 17;337(6096):816-21. (PMID: 22745249)
Stem Cell Reports. 2015 Sep 8;5(3):448-59. (PMID: 26352799)
Cell. 2014 Mar 27;157(1):77-94. (PMID: 24679528)
Nat Rev Microbiol. 2015 Nov;13(11):722-36. (PMID: 26411297)
EMBO J. 1988 May;7(5):1395-402. (PMID: 3409869)
Front Mol Neurosci. 2018 Aug 15;11:282. (PMID: 30158855)
Plant Biotechnol J. 2019 Oct;17(10):1905-1913. (PMID: 30839150)
Biochem Biophys Res Commun. 2014 Aug 8;450(4):1422-6. (PMID: 25044113)
Nucleic Acids Res. 2014 Apr;42(7):4375-90. (PMID: 24500196)
Nature. 1998 Jul 23;394(6691):369-74. (PMID: 9690471)
Nat Commun. 2015 Jun 11;6:7391. (PMID: 26067104)
Sci Rep. 2014 Jun 23;4:5396. (PMID: 24953798)
Curr Opin Genet Dev. 2013 Apr;23(2):124-31. (PMID: 23541525)
Cell Stem Cell. 2013 Dec 5;13(6):653-8. (PMID: 24315439)
Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14628-33. (PMID: 9843940)
Nat Methods. 2006 Sep;3(9):707-14. (PMID: 16929316)
Science. 2007 Dec 21;318(5858):1917-20. (PMID: 18029452)
معلومات مُعتمدة: NRF-2019M3A9A8065098, 2020R1F1A1071517, 2019M3D1A1078940, and 2019R1A6A1A11051471 National Research Foundation of Korea
فهرسة مساهمة: Keywords: CRISPR activator; CRISPR/dCas9; gene activation; reprogramming factor
المشرفين على المادة: 0 (Octamer Transcription Factor-3)
0 (POU5F1 protein, human)
0 (RNA, Messenger)
0 (SOX2 protein, human)
0 (SOXB1 Transcription Factors)
EC 3.1.- (CRISPR-Associated Protein 9)
تواريخ الأحداث: Date Created: 20210106 Date Completed: 20210324 Latest Revision: 20210324
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC7795359
DOI: 10.3390/ijms22010397
PMID: 33401508
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms22010397