دورية أكاديمية
أعرض في EDS

Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation.

التفاصيل البيبلوغرافية
العنوان: Skin Equivalent Models: Protocols for In Vitro Reconstruction for Dermal Toxicity Evaluation.
المؤلفون: do Nascimento Pedrosa T; Skin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil., Catarino CM; Skin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil., Pennacchi PC; Skin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil., de Moraes Barros SB; Skin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil., Maria-Engler SS; Skin Biology and Melanoma Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Butantã, SP, Brazil. silvya@usp.br.
المصدر: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2240, pp. 31-41.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
مواضيع طبية MeSH: Epidermis/*drug effects , Primary Cell Culture/*methods , Skin Irritancy Tests/*methods, Cells, Cultured ; Humans
مستخلص: This chapter presents the protocols for developing of skin equivalents (SE) and reconstructed human epidermis (RHE) models for dermal toxicity evaluation as an alternative method to animal use in research. It provides a detailed protocol for the in vitro reconstruction of human skin from primary keratinocytes, melanocytes, and fibroblasts obtained from foreskin biopsies, including the procedures for reconstruction of a stratified epidermis on a polyester membrane. SE and RHE developed through these methods have been proven suitable not only for dermal toxicity studies, but also for investigating of pathological conditions in the skin, such as diabetes and invasion of melanoma.
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فهرسة مساهمة: Keywords: Human primary cells; Primary culture; Reconstructed human epidermis (RHE); Skin equivalent (SE)
تواريخ الأحداث: Date Created: 20210110 Date Completed: 20210326 Latest Revision: 20210326
رمز التحديث: 20240628
DOI: 10.1007/978-1-0716-1091-6_3
PMID: 33423224
قاعدة البيانات: MEDLINE
الوصف
تدمد:1940-6029
DOI:10.1007/978-1-0716-1091-6_3