دورية أكاديمية
Determination of chemical structure and anti- Trypanosoma cruzi activity of extracts from the roots of Lonchocarpus cultratus (Vell.) A.M.G. Azevedo & H.C. Lima.
العنوان: | Determination of chemical structure and anti- Trypanosoma cruzi activity of extracts from the roots of Lonchocarpus cultratus (Vell.) A.M.G. Azevedo & H.C. Lima. |
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المؤلفون: | Bortoluzzi AAM; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., Staffen IV; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., Banhuk FW; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., Griebler A; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., Matos PK; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., Ayala TS; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., da Silva EAA; Center of Exact Sciences and Technology, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., Sarragiotto MH; Center of Exact Sciences, Department of Chemistry, State University of Maringa (UEM), 5790 Colombo Av., Maringa, PR, Brazil., Schuquel ITA; Center of Exact Sciences, Department of Chemistry, State University of Maringa (UEM), 5790 Colombo Av., Maringa, PR, Brazil., Jorge TCM; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil., Menolli RA; Center of Medical and Pharmaceutical Sciences, Western Parana State University (UNIOESTE), 2069 Universitaria St., Cascavel, PR, Brazil. |
المصدر: | Saudi journal of biological sciences [Saudi J Biol Sci] 2021 Jan; Vol. 28 (1), pp. 99-108. Date of Electronic Publication: 2020 Sep 01. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Saudi Biological Society Country of Publication: Saudi Arabia NLM ID: 101543796 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1319-562X (Print) Linking ISSN: 22137106 NLM ISO Abbreviation: Saudi J Biol Sci Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Riyadh : Saudi Biological Society : King Saud University, [2009]- |
مستخلص: | Trypanosoma cruzi is the agent of Chagas disease, an infection that affects around 8 million people worldwide. The search for new anti- T. cruzi drugs are relevant, mainly because the treatment of this disease is limited to two drugs. The objective of this study was to investigate the trypanocidal and cytotoxic activity and elucidate the chemical profile of extracts from the roots of the Lonchocarpus cultratus . Roots from L. cultratus were submitted to successive extractions with hexane, dichloromethane, and methanol, resulting in LCH, LCD, and LCM extracts, respectively. Characterization of extracts was done using 1 H-RMN, 13 C-RMN, CC and TLC. Treatment of T. cruzi forms (epimastigotes, trypomastigotes, and amastigotes) with crescent concentrations of LCH, LCD, and LCM was done for 72, 48, and 48 h, respectively. After this, the percentage of inhibition and IC Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2020 The Authors.) |
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فهرسة مساهمة: | Keywords: ANOVA, Analysis of Variance; BZN, Benznidazole; CC, column chromatography; CC50, Cytotoxic Concentration 50%; CDCl3, Deuterate chloroform; CO2, Carbon dioxide; Chagas disease; Chalcones; DC, DMSO Control; DMSO, Dimethyl Sulfoxide; FBS, Fetal Bovine Serum; IC50, Inhibitory Concentration 50%; LC-1, 2 and 3: Fractions obtained from LCH extract; LC-4 and 5, fractions obtained from LCD extract; LC50, Lethal Concentration 50%; LCD, Extract from L. cultratus obtained by extraction with dichloromethane; LCH, Extract from L. cultratus obtained by extraction with hexane; LCM, Extract from L. cultratus obtained by extraction with methanol; LIT, Liver Infusion Tryptose; Lafepe, Pharmaceutical Laboratory of Pernambuco State; Lonchocarpus; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; NMR, Nuclear Magnetic Resonance; NO, Nitric Oxide; PBS, Phosphate-Buffered Saline; Plant extract; RPMI, Roswell Park Memorial Institute; SI, Selectivity Index; TLC, Thin Layer column; TMS, Tetramethylsilane; Trypanosomiasis; UC, Untreated Control; UEM, State University of Maringa/PR |
تواريخ الأحداث: | Date Created: 20210111 Latest Revision: 20240404 |
رمز التحديث: | 20240404 |
مُعرف محوري في PubMed: | PMC7783657 |
DOI: | 10.1016/j.sjbs.2020.08.036 |
PMID: | 33424286 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1319-562X |
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DOI: | 10.1016/j.sjbs.2020.08.036 |