دورية أكاديمية
أعرض في EDS

Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomes.

التفاصيل البيبلوغرافية
العنوان: Host transcriptomic profiling of COVID-19 patients with mild, moderate, and severe clinical outcomes.
المؤلفون: Jain R; Al Jalila Genomics Center, Al Jalila Children's Hospital, Dubai, United Arab Emirates., Ramaswamy S; Al Jalila Genomics Center, Al Jalila Children's Hospital, Dubai, United Arab Emirates., Harilal D; Al Jalila Genomics Center, Al Jalila Children's Hospital, Dubai, United Arab Emirates., Uddin M; College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates.; The Centre for Applied Genomics (TCAG), The Hospital for Sick Children, Toronto, ON, Canada., Loney T; College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates., Nowotny N; College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates.; Institute of Virology, University of Veterinary Medicine Vienna, Vienna, Austria., Alsuwaidi H; College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates., Varghese R; Microbiology and Infection Control Unit, Pathology and Genetics Department, Latifa Women and Children Hospital, Dubai Health Authority, Dubai, United Arab Emirates., Deesi Z; Microbiology and Infection Control Unit, Pathology and Genetics Department, Latifa Women and Children Hospital, Dubai Health Authority, Dubai, United Arab Emirates., Alkhajeh A; Medical Education & Research Department, Dubai Health Authority, Dubai, United Arab Emirates., Khansaheb H; Medical Education & Research Department, Dubai Health Authority, Dubai, United Arab Emirates., Alsheikh-Ali A; College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates., Abou Tayoun A; Al Jalila Genomics Center, Al Jalila Children's Hospital, Dubai, United Arab Emirates.; College of Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates.
المصدر: Computational and structural biotechnology journal [Comput Struct Biotechnol J] 2020 Dec 17; Vol. 19, pp. 153-160. Date of Electronic Publication: 2020 Dec 17 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology Country of Publication: Netherlands NLM ID: 101585369 Publication Model: eCollection Cited Medium: Print ISSN: 2001-0370 (Print) Linking ISSN: 20010370 NLM ISO Abbreviation: Comput Struct Biotechnol J Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology
Original Publication: Gothenburg, Sweden : Research Network of Computational and Structural Biotechnology
مستخلص: Characterizing key molecular and cellular pathways involved in COVID-19 is essential for disease prognosis and management. We perform shotgun transcriptome sequencing of human RNA obtained from nasopharyngeal swabs of patients with COVID-19, and identify a molecular signature associated with disease severity. Specifically, we identify globally dysregulated immune related pathways, such as cytokine-cytokine receptor signaling, complement and coagulation cascades, JAK-STAT, and TGF- β signaling pathways in all, though to a higher extent in patients with severe symptoms. The excessive release of cytokines and chemokines such as CCL2 , CCL22 , CXCL9 and CXCL12 and certain interferons and interleukins related genes like IFIH1 , IFI44 , IFIT1 and IL10 were significantly higher in patients with severe clinical presentation compared to mild and moderate presentations. Differential gene expression analysis identified a small set of regulatory genes that might act as strong predictors of patient outcome. Our data suggest that rapid transcriptome analysis of nasopharyngeal swabs can be a powerful approach to quantify host molecular response and may provide valuable insights into COVID-19 pathophysiology.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2020 The Author(s).)
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فهرسة مساهمة: Keywords: COVID-19; Disease severity; Expression signature; Nasopharyngeal swabs; Transcriptome sequencing
تواريخ الأحداث: Date Created: 20210111 Latest Revision: 20240330
رمز التحديث: 20240330
مُعرف محوري في PubMed: PMC7773686
DOI: 10.1016/j.csbj.2020.12.016
PMID: 33425248
قاعدة البيانات: MEDLINE
الوصف
تدمد:2001-0370
DOI:10.1016/j.csbj.2020.12.016