دورية أكاديمية
Identification and characterization of resistance and pathogenicity of Enterococcus spp . in samples of donor breast milk.
| العنوان: | Identification and characterization of resistance and pathogenicity of Enterococcus spp . in samples of donor breast milk. |
|---|---|
| المؤلفون: | Santana LAM; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., Andrade NNN; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., da Silva LSC; Campus Soane Nazaré de Andrade, Universidade Estadual de Santa Cruz, Ilhéus 45662-900, Bahia, Brazil., Oliveira CNT; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., de Brito BB; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., de Melo FF; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., Souza CL; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., Marques LM; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil., Oliveira MV; Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia, Vitória da Conquista 45029-094, Bahia, Brazil.freiremelo@yahoo.com.br. |
| المصدر: | World journal of clinical pediatrics [World J Clin Pediatr] 2020 Nov 19; Vol. 9 (3), pp. 53-62. Date of Electronic Publication: 2020 Nov 19 (Print Publication: 2020). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Baishideng Publishing Group Country of Publication: United States NLM ID: 101627548 Publication Model: eCollection Cited Medium: Print ISSN: 2219-2808 (Print) Linking ISSN: 22192808 NLM ISO Abbreviation: World J Clin Pediatr Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2014- : Pleasanton, CA : Baishideng Publishing Group Original Publication: Hong Kong, China : Baishideng Publishing Group Co., Limited, [2012]- |
| مستخلص: | Background: Breast milk is the primary source of nutrition for newborns. Hospitalized babies frequently need nutritional support from Human Milk Banks. As bacterial species of the genus Enterococcus are part of the microbiota of healthy donors, they may contaminate samples of pumped breast milk. Aim: To identify and characterize the bacterial virulence and resistance in samples isolated from the nipple-areolar region, hands, and breast milk aliquots from donors at the Human Milk Bank of Municipal Hospital Esaú Matos in the city of Vitória da Conquista, Bahia State, Brazil. Methods: The personal hygiene and sanitation of donors were analyzed with the aim of identifying possible reasons for contamination of pumped milk. Cutaneous samples as well as aliquots of unpasteurized and pasteurized milk from 30 participants were obtained. Each Enterococcus spp . isolate underwent a disk diffusion susceptibility test and molecular biology techniques to determine resistance and virulence genes. Results: Enterococcus spp . were identified in 30% of donors ( n = 9), and 11 specimens were isolated. Resistance to tetracycline was highly prevalent, being detectable in 63% of the isolates ( n = 7) and followed by intermediate sensitivity to ciprofloxacin, observed in 27% of the specimens ( n = 3). The efa A gene was found in 63% ( n = 7) of the isolates, while the ace gene was detected in 27% ( n = 3). Conclusion: This study illustrates the importance of microbiological monitoring by Human Milk Banks and the need for alternatives to prevent the presence of Enterococcus spp . in hospital settings. Competing Interests: Conflict-of-interest statement: The authors of this manuscript have no conflicts of interest to disclose. (©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.) |
| References: | Braz J Infect Dis. 2014 Mar-Apr;18(2):129-36. (PMID: 24216155) J Pediatr (Rio J). 2004 Nov;80(5 Suppl):S119-25. (PMID: 15583761) Eur J Clin Microbiol Infect Dis. 2014 Jun;33(6):933-9. (PMID: 24463723) J Clin Microbiol. 1995 Jan;33(1):24-7. (PMID: 7699051) Antimicrob Agents Chemother. 1999 Apr;43(4):947-9. (PMID: 10103206) J Clin Microbiol. 2004 Oct;42(10):4473-9. (PMID: 15472296) Rev Saude Publica. 2003 Dec;37(6):775-9. (PMID: 14666308) Appl Environ Microbiol. 2001 Apr;67(4):1628-35. (PMID: 11282615) Mem Inst Oswaldo Cruz. 2007 Nov;102(7):853-9. (PMID: 18060316) Antimicrob Agents Chemother. 1999 Oct;43(10):2513-6. (PMID: 10508034) J Pediatr. 2003 Dec;143(6):754-8. (PMID: 14657823) J Pediatr (Rio J). 2018 Jul - Aug;94(4):399-403. (PMID: 28859913) J Pediatr (Rio J). 2019 May - Jun;95(3):298-305. (PMID: 29530663) Food Microbiol. 2010 Oct;27(7):955-61. (PMID: 20688238) Int J Food Microbiol. 2003 Dec 1;88(2-3):291-304. (PMID: 14597001) Appl Environ Microbiol. 2002 Mar;68(3):1381-91. (PMID: 11872491) Res Microbiol. 2007 Jan-Feb;158(1):31-7. (PMID: 17224259) Cad Saude Publica. 2001 May-Jun;17(3):713-7. (PMID: 11395808) Food Microbiol. 2008 Aug;25(5):668-75. (PMID: 18541165) Infect Immun. 1999 Jan;67(1):193-200. (PMID: 9864215) |
| فهرسة مساهمة: | Keywords: Antimicrobial resistance; Breast milk; Enterococcus spp.; Human milk; Pasteurization; Virulence |
| تواريخ الأحداث: | Date Created: 20210114 Latest Revision: 20210115 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7769778 |
| DOI: | 10.5409/wjcp.v9.i3.53 |
| PMID: | 33442535 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2219-2808 |
|---|---|
| DOI: | 10.5409/wjcp.v9.i3.53 |