دورية أكاديمية
أعرض في EDS

Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?

التفاصيل البيبلوغرافية
العنوان: Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?
المؤلفون: Tozetto-Mendoza TR; LIM-52, Institute of Tropical Medicine, School of Medicine, Clinical Hospital, University of São Paulo, Brazil. Electronic address: tozetto@usp.br., Kanunfre KA; LIM-48, Clinical Hospital, School of Medicine, University of São Paulo, Brazil; Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, School of Medicine, University of São Paulo, Brazil., Vilas-Boas LS; LIM-52, Institute of Tropical Medicine, School of Medicine, Clinical Hospital, University of São Paulo, Brazil., Sanchez Espinoza EP; LIM-49, Institute of Tropical Medicine, School of Medicine, University of São Paulo, Brazil; Department of Infection Diseases, Clinical Hospital, School of Medicine, University of São Paulo, Brazil., Paião HGO; LIM-52, Institute of Tropical Medicine, School of Medicine, Clinical Hospital, University of São Paulo, Brazil; Department of Infection Diseases, Clinical Hospital, School of Medicine, University of São Paulo, Brazil., Rocha MC; LIM-48, Clinical Hospital, School of Medicine, University of São Paulo, Brazil; Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, School of Medicine, University of São Paulo, Brazil., de Paula AV; LIM-52, Institute of Tropical Medicine, School of Medicine, Clinical Hospital, University of São Paulo, Brazil., de Oliveira MS; Department of Infection control- Clinical Hospital, School of Medicine, University of São Paulo, Brazil; Hospital Sírio Libanês, Brazil., Zampelli DB; Hospital Sírio Libanês, Brazil., Vieira JM Jr; Hospital Sírio Libanês, Brazil., Buss L; Department of Infection Diseases, Clinical Hospital, School of Medicine, University of São Paulo, Brazil., Costa SF; LIM-49, Institute of Tropical Medicine, School of Medicine, University of São Paulo, Brazil; Department of Infection Diseases, Clinical Hospital, School of Medicine, University of São Paulo, Brazil., Sabino EC; Department of Infection Diseases, Clinical Hospital, School of Medicine, University of São Paulo, Brazil; LIM-46, Institute of Tropical Medicine, School of Medicine, University of São Paulo, Brazil., Witkin SS; LIM-52, Institute of Tropical Medicine, School of Medicine, Clinical Hospital, University of São Paulo, Brazil; Department of Obstetrics and Gynecology, Weill Cornell Medicine, New York, New York, United States., Okay TS; Laboratory of Seroepidemiology and Immunobiology, Institute of Tropical Medicine, School of Medicine, University of São Paulo, Brazil; Department of Pediatrics, School of Medicine, University of São Paulo, Brazil., Mendes-Correa MC; LIM-52, Institute of Tropical Medicine, School of Medicine, Clinical Hospital, University of São Paulo, Brazil; Department of Infection Diseases, Clinical Hospital, School of Medicine, University of São Paulo, Brazil.
المصدر: Journal of virological methods [J Virol Methods] 2021 Apr; Vol. 290, pp. 114064. Date of Electronic Publication: 2021 Jan 13.
نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980-
مواضيع طبية MeSH: COVID-19 Serological Testing* , Enzyme-Linked Immunosorbent Assay*, COVID-19/*diagnosis , Coronavirus Nucleocapsid Proteins/*immunology , Immunoglobulin G/*blood , SARS-CoV-2/*isolation & purification, Adult ; Aged ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Female ; Humans ; Immunoglobulin G/immunology ; Male ; Middle Aged ; Phosphoproteins/immunology ; SARS-CoV-2/immunology ; Sensitivity and Specificity
مستخلص: Objectives: We evaluated the performance of a nucleoprotein-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies to SARS-CoV-2.
Methods: The ELISA was based on serum IgG reactivity to a 46-kDa protein derived from the recombinant SARS-CoV2 nucleoprotein. Assay sensitivity was assessed using serum samples from 134 COVID-19 confirmed cases obtained > 15 days after symptom onset. Specificity was determined by testing sera from 94 healthy controls. Cross-reactivity was evaluated with sera from 96 individuals with previous dengue or zika virus-confirmed infections, with 44 sera from individuals with confirmed infections to other respiratory viruses or with bacterial and fungal infections that cause pneumonia and with 40 sera negative for SARS-CoV-2 nucleoprotein by commercial ELISA kits.
Results: The majority of subjects were male and ≥ 60 years old. Assay sensitivity was 90.3 % (95 % confidence interval 84.1 %-94.2 %) and specificity was 97.9 % (92.6 %-99.4 %). There was no cross-reactivity with sera from individuals diagnosed with dengue, zika virus, influenza virus, rhinovirus, adenovirus, respiratory syncytial virus, seasonal coronavirus, Mycobacterium tuberculosis, Staphylococcus (S. aureus and coagulase-negative), Streptococcus pneumoniae, Klebsiella pneumoniae and the fungus Aspergillus fumigatus. The level of concordance of our test with results from commercial ELISA kits was 100 %.
Conclusion: The nucleoprotein-based ELISA was specific for detection of IgG anti-nucleoprotein antibodies to SARS-CoV-2. It utilizes a frequently employed low expense assay protocol and is easier to perform than other currently available commercial SARS-CoV2 antibody detection tests.
(Copyright © 2021. Published by Elsevier B.V.)
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فهرسة مساهمة: Keywords: COVID-19; ELISA; IgG antibody; Nucleoprotein antigen; SARS-CoV-2
المشرفين على المادة: 0 (Antibodies, Viral)
0 (Coronavirus Nucleocapsid Proteins)
0 (Immunoglobulin G)
0 (Phosphoproteins)
0 (nucleocapsid phosphoprotein, SARS-CoV-2)
تواريخ الأحداث: Date Created: 20210116 Date Completed: 20210325 Latest Revision: 20221216
رمز التحديث: 20221216
مُعرف محوري في PubMed: PMC7804377
DOI: 10.1016/j.jviromet.2021.114064
PMID: 33453299
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0984
DOI:10.1016/j.jviromet.2021.114064