دورية أكاديمية
Gelatin-Based 3D Microgels for In Vitro T Lineage Cell Generation.
| العنوان: | Gelatin-Based 3D Microgels for In Vitro T Lineage Cell Generation. |
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| المؤلفون: | Suraiya AB; Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Wellington Road, Clayton, Melbourne 3800, Australia.; Department of Anatomy and Developmental Biology, Biomedicine Discovery Institute, Monash University, Wellington Road, Clayton, Melbourne 3800, Australia., Hun ML; Department of Anatomy and Developmental Biology, Biomedicine Discovery Institute, Monash University, Wellington Road, Clayton, Melbourne 3800, Australia., Truong VX; Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Wellington Road, Clayton, Melbourne 3800, Australia., Forsythe JS; Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Wellington Road, Clayton, Melbourne 3800, Australia., Chidgey AP; Department of Anatomy and Developmental Biology, Biomedicine Discovery Institute, Monash University, Wellington Road, Clayton, Melbourne 3800, Australia. |
| المصدر: | ACS biomaterials science & engineering [ACS Biomater Sci Eng] 2020 Apr 13; Vol. 6 (4), pp. 2198-2208. Date of Electronic Publication: 2020 Mar 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101654670 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2373-9878 (Electronic) Linking ISSN: 23739878 NLM ISO Abbreviation: ACS Biomater Sci Eng Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, [2015]- |
| مواضيع طبية MeSH: | Cell Differentiation* , Gelatin* , Microgels* , T-Lymphocytes*, Animals ; Epithelial Cells ; Mice |
| مستخلص: | T cells are predominantly produced by the thymus and play a significant role in maintaining our adaptive immune system. Physiological involution of the thymus occurs gradually with age, compromising naive T cell output, which can have severe clinical complications. Also, T cells are utilized as therapeutic agents in cancer immunotherapies. Therefore, there is an increasing need for strategies aimed at generating naive T cells. The majority of in vitro T cell generation studies are performed in two-dimensional (2D) cultures, which ignore the physiological thymic microenvironment and are not scalable; therefore, we applied a new three-dimensional (3D) approach. Here, we use a gelatin-based 3D microgel system for T lineage induction by co-culturing OP9-DL4 cells and mouse fetal-liver-derived hematopoietic stem cells (HSCs). Flow cytometric analysis revealed that microgel co-cultures supported T lineage induction similar to 2D cultures while providing a 3D environment. We also encapsulated mouse embryonic thymic epithelial cells (TECs) within the microgels to provide a defined 3D culture platform. The microgel system supported TEC maintenance and retained their phenotype. Together, these data show that our microgel system has the capacity for TEC maintenance and induction of in vitro T lineage differentiation with potential for scalability. |
| فهرسة مساهمة: | Keywords: 3D co-cultures; OP9-DL4 cell line; T cells; cell encapsulation; microgels; thymic epithelial cells |
| المشرفين على المادة: | 0 (Microgels) 9000-70-8 (Gelatin) |
| تواريخ الأحداث: | Date Created: 20210118 Date Completed: 20210510 Latest Revision: 20210510 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1021/acsbiomaterials.9b01610 |
| PMID: | 33455336 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2373-9878 |
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| DOI: | 10.1021/acsbiomaterials.9b01610 |