دورية أكاديمية
Global mapping of translation initiation sites by TIS profiling in budding yeast.
| العنوان: | Global mapping of translation initiation sites by TIS profiling in budding yeast. |
|---|---|
| المؤلفون: | Hollerer I; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.; California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, CA 94158, USA., Powers EN; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.; California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, CA 94158, USA., Brar GA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.; California Institute for Quantitative Biosciences (QB3), University of California, San Francisco, CA 94158, USA.; Center for Computational Biology, University of California, Berkeley, Berkeley, CA 94720, USA. |
| المصدر: | STAR protocols [STAR Protoc] 2020 Dec 30; Vol. 2 (1), pp. 100250. Date of Electronic Publication: 2020 Dec 30 (Print Publication: 2021). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101769501 Publication Model: eCollection Cited Medium: Internet ISSN: 2666-1667 (Electronic) Linking ISSN: 26661667 NLM ISO Abbreviation: STAR Protoc |
| أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2020]- |
| مواضيع طبية MeSH: | Codon, Initiator* , Open Reading Frames* , Peptide Chain Initiation, Translational* , Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae Proteins*/biosynthesis , Saccharomyces cerevisiae Proteins*/genetics, Ribosomes/*metabolism, Ribosomes/genetics |
| مستخلص: | Translation initiation site (TIS) profiling allows for the genome-wide identification of TISs in vivo by exclusively capturing mRNA fragments within ribosomes that have just completed translation initiation. It leverages translation inhibitors, such as harringtonine and lactimidomycin (LTM), that preferentially capture ribosomes at start codon positions, protecting TIS-derived mRNA fragments from nuclease digestion. Here, we describe a step-by-step protocol for TIS profiling in LTM-treated budding yeast that we developed to identify TISs and open reading frames in vegetative and meiotic cells. For complete details on the use and execution of this protocol, please refer to Eisenberg et al. (2020). Competing Interests: The authors declare no competing interests. (© 2020 The Author(s).) |
| References: | Nat Chem Biol. 2010 Mar;6(3):209-217. (PMID: 20118940) Methods Mol Biol. 2021;2252:89-125. (PMID: 33765272) Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):E2424-32. (PMID: 22927429) Cell Syst. 2020 Aug 26;11(2):145-160.e5. (PMID: 32710835) Mol Cell. 2015 Dec 3;60(5):816-827. (PMID: 26638175) Nat Protoc. 2012 Jul 26;7(8):1534-50. (PMID: 22836135) Nat Rev Mol Cell Biol. 2015 Nov;16(11):651-64. (PMID: 26465719) |
| معلومات مُعتمدة: | DP2 GM119138 United States GM NIGMS NIH HHS; R35 GM134886 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: Cell biology; Genomics; High-throughput screening; Model organisms; Molecular biology |
| المشرفين على المادة: | 0 (Codon, Initiator) 0 (Saccharomyces cerevisiae Proteins) |
| تواريخ الأحداث: | Date Created: 20210118 Date Completed: 20220317 Latest Revision: 20240330 |
| رمز التحديث: | 20240330 |
| مُعرف محوري في PubMed: | PMC7797918 |
| DOI: | 10.1016/j.xpro.2020.100250 |
| PMID: | 33458709 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2666-1667 |
|---|---|
| DOI: | 10.1016/j.xpro.2020.100250 |