Effects of Ruscus extract on muscarinic receptors: Is there a role for endothelium derived relaxing factors on macromolecular permeability protection and microvascular diameter changes?

التفاصيل البيبلوغرافية
العنوان:	Effects of Ruscus extract on muscarinic receptors: Is there a role for endothelium derived relaxing factors on macromolecular permeability protection and microvascular diameter changes?
المؤلفون:	de Souza MDGC; Laboratório de Pesquisas Clínicas e Experimentais em Biologia Vascular, Centro Biomédico, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Cyrino FZGA; Laboratório de Pesquisas Clínicas e Experimentais em Biologia Vascular, Centro Biomédico, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Sicuro FL; Laboratório de Pesquisas Clínicas e Experimentais em Biologia Vascular, Centro Biomédico, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Bouskela E; Laboratório de Pesquisas Clínicas e Experimentais em Biologia Vascular, Centro Biomédico, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
المصدر:	Clinical hemorheology and microcirculation [Clin Hemorheol Microcirc] 2021; Vol. 77 (4), pp. 443-459.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Ios Press Country of Publication: Netherlands NLM ID: 9709206 Publication Model: Print Cited Medium: Internet ISSN: 1875-8622 (Electronic) Linking ISSN: 13860291 NLM ISO Abbreviation: Clin Hemorheol Microcirc Subsets: MEDLINE
أسماء مطبوعة:	Publication: Amsterdam : Ios Press Original Publication: Amsterdam ; Washington, DC : IOS Press, c1997-
مواضيع طبية MeSH:	Endothelial Cells/metabolism , Endothelium-Dependent Relaxing Factors/therapeutic use , Plant Extracts/chemistry , Receptors, Muscarinic/chemistry , Ruscus/*chemistry, Animals ; Endothelium-Dependent Relaxing Factors/pharmacology ; Male ; Mesocricetus ; Nitric Oxide/pharmacology
مستخلص:	Background: Protective effects of Ruscus extract on macromolecular permeability depend on its capacity to stimulate muscarinic receptors on endothelial cells and induce the release of endothelium derived relaxing factors (EDRFs). Objective: To investigate if these effects depend only on activation of muscarinic receptors or if EDRFs release are also necessary. We have also investigated the participation of Ruscus extract on muscarinic-induced release of EDRFs on microvascular diameters. Methods: Hamsters were treated daily during two weeks with Ruscus extract (50, 150 and 450 mg/kg/day) and then macromolecular permeability induced by histamine and arteriolar and venular diameters after cyclooxygenase (COX) and nitric oxide synthase (NOS) inhibitors: indomethacin and Nω-Nitro-L-arginine (LNA), respectively applied topically at 10-8M, 10-6M and 10-4M were observed on the cheek pouch preparation. Results: Ruscus extract decreased macromolecular permeability in a dose-dependent fashion and did not affect microvascular diameters. NOS and COX inhibitors enhanced its effect on microvascular permeability. NOS inhibition reduced arteriolar diameter and COX blocking decreased arteriolar and venular diameters at the lowest dose and increased them at higher doses of Ruscus extract. Conclusion: The protective effect of Ruscus extract on macromolecular permeability seems to be mediated only via muscarinic receptors. Muscarinic activation attenuated vasoconstrictive tone through cyclooxygenase-independent endothelium derived relaxing factors.
فهرسة مساهمة:	Keywords: Ruscus extract; cyclooxygenase; endothelium derived relaxing factors; hamster cheek pouch preparation; macromolecular permeability; microvascular diameter; muscarinic receptors; nitric oxide synthase
المشرفين على المادة:	0 (Endothelium-Dependent Relaxing Factors) 0 (Plant Extracts) 0 (Receptors, Muscarinic) 31C4KY9ESH (Nitric Oxide)
تواريخ الأحداث:	Date Created: 20210118 Date Completed: 20210521 Latest Revision: 20210521
رمز التحديث:	20221213
DOI:	10.3233/CH-201019
PMID:	33459701
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1875-8622
DOI:	10.3233/CH-201019