دورية أكاديمية
Chemopreventive Effect of 5-Flurouracil Polymeric Hybrid PLGA-Lecithin Nanoparticles against Colon Dysplasia Model in Mice and Impact on p53 Apoptosis.
العنوان: | Chemopreventive Effect of 5-Flurouracil Polymeric Hybrid PLGA-Lecithin Nanoparticles against Colon Dysplasia Model in Mice and Impact on p53 Apoptosis. |
---|---|
المؤلفون: | A Attia M; Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.; Department of Pharmacology, College of Medicine, AlMaarefa University, Riyadh 11597, Saudi Arabia., Enan ET; Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt., Hashish AA; Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt.; Basic Medical Sciences Department, College of Medicine, University of Bisha, Bisha 61922, Saudi Arabia., M H El-Kannishy S; Department of Toxicology, Mansoura Hospital, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.; Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia., Gardouh AR; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.; Department of Pharmacy, Faculty of Pharmacy, Jadara University, Irbid 21110, Jordan., K Tawfik M; Department of Clinical Pharmacology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt., Faisal S; Department of Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia 41522, Egypt., El-Mistekawy A; Department of Internal Medicine, Gastroenterology Division, Faculty of Medicine, Al-Azhar University, Cairo 11651, Egypt., Salama A; Department of Pharmaceutics, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia.; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo 11751, Egypt., Alomar SY; Doping Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh 11495, Saudi Arabia., H Eltrawy A; Department of Anatomy and Embryology, Faculty of Medicine, Alexandria University, Alexandria 22785, Egypt., Yagub Aloyouni S; Health Sciences Research Center, Princess Nourah Bint Abdulrahman University, Riyadh 84428, Saudi Arabia., Zaitone SA; Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia.; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. |
المصدر: | Biomolecules [Biomolecules] 2021 Jan 15; Vol. 11 (1). Date of Electronic Publication: 2021 Jan 15. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101596414 Publication Model: Electronic Cited Medium: Internet ISSN: 2218-273X (Electronic) Linking ISSN: 2218273X NLM ISO Abbreviation: Biomolecules Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, 2011- |
مواضيع طبية MeSH: | Chemoprevention*, Fluorouracil/*pharmacology , Lecithins/*chemistry , Nanoparticles/*chemistry , Polylactic Acid-Polyglycolic Acid Copolymer/*chemistry , Polymers/*chemistry, Animals ; Apoptosis ; Colon/drug effects ; Colon/pathology ; Disease Models, Animal ; Drug Liberation ; Ki-67 Antigen/metabolism ; Lipids/chemistry ; Male ; Mice ; Particle Size ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Static Electricity ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism |
مستخلص: | The use of 5-fluorouracil (5FU) is associated with multifaceted challenges and poor pharmacokinetics. Poly(lactic-co-glycolic acid)-lipid hybrid nanoparticles (PLNs)-based therapy has received attention as efficient carriers for a diversity of drugs. This study evaluated the in vivo chemotherapeutic and anti-proliferative efficacy of 5FU-loaded PLNs against 1,2-dimethylhydrazine (Di-MH) prompted colon dysplasia in mice compared to free 5FU. 5FU PLNs were prepared. Male Swiss albino mice were distributed to six experimental groups. Group 1: Saline group. All the other groups were injected weekly with Di-MH [20 mg/kg, s.c.]. Group 2: Di-MH induced colon dysplasia control group. Groups 3 and 4: Di-MH + free 5FU treated group [2.5 and 5 mg/kg]. Groups 5 and 6: Di-MH + 5FU-PLNs treated group [2.5 and 5 mg/kg]. Free 5FU and 5FU-PLNs doses were administered orally, twice weekly. Treatment with 5FU-PLNs induced a higher cytoprotective effect compared to free 5FU as indicated by lower mucosal histopathologic score and reduction in number of Ki-67 immunpositive proliferating nuclei. Additionally, there was significant upregulation of p53 and caspase 3 genes in colon specimens. Our results support the validity of utilizing the PLNs technique to improve the chemopreventive action of 5FU in treating colon cancer. |
References: | Eur J Pharm Biopharm. 2013 Nov;85(3 Pt A):427-43. (PMID: 23872180) EMBO J. 2016 Feb 15;35(4):402-13. (PMID: 26783364) Cancer Sci. 2004 Jun;95(6):475-80. (PMID: 15182426) Nutr Cancer. 2013;65(1):92-8. (PMID: 23368918) PLoS One. 2015 Sep 14;10(9):e0137888. (PMID: 26368019) Int J Nanomedicine. 2014 Feb 21;9:1083-96. (PMID: 24591829) Nanomedicine. 2013 May;9(4):474-91. (PMID: 23261500) Trends Biotechnol. 2017 Aug;35(8):770-784. (PMID: 28645529) Gastroenterology. 2020 Jul;159(1):335-349.e15. (PMID: 32247694) Phytother Res. 2013 Jul;27(7):999-1005. (PMID: 22899565) Biomed Pharmacother. 2016 Aug;82:106-16. (PMID: 27470345) Int J Pharm. 2011 Jun 30;412(1-2):132-41. (PMID: 21507344) J Control Release. 2014 Dec 10;195:63-71. (PMID: 25108151) PLoS One. 2012;7(10):e47115. (PMID: 23077553) Curr Opin Cell Biol. 2001 Jun;13(3):332-7. (PMID: 11343904) Sci Rep. 2019 Aug 23;9(1):12288. (PMID: 31444363) Cancer Chemother Pharmacol. 2011 Dec;68(6):1449-57. (PMID: 21479885) Toxicol Res (Camb). 2020 Mar 30;9(1):2-18. (PMID: 32440334) Chem Biol Interact. 2018 Nov 1;295:52-63. (PMID: 29678497) Int J Nanomedicine. 2011;6:1685-97. (PMID: 21980233) J Pharmacol Exp Ther. 2006 Jun;317(3):1372-81. (PMID: 16547167) Biomater Res. 2016 Oct 31;20:34. (PMID: 27807476) Front Pharmacol. 2018 Nov 02;9:1260. (PMID: 30450050) Oncogene. 2013 Sep 19;32(38):4529-38. (PMID: 23108402) Int J Nanomedicine. 2017 Mar 08;12:1853-1862. (PMID: 28331310) Artif Cells Nanomed Biotechnol. 2016;44(1):334-49. (PMID: 25237838) PLoS One. 2014 Jun 27;9(6):e100562. (PMID: 24971882) Int J Nanomedicine. 2019 Jul 05;14:4961-4974. (PMID: 31308666) Molecules. 2015 Jan 08;20(1):879-99. (PMID: 25580685) Expert Opin Drug Deliv. 2016;13(5):691-707. (PMID: 26866382) J Carcinog. 2009;8:5. (PMID: 19332896) Surg Clin North Am. 2002 Oct;82(5):891-904. (PMID: 12507199) AAPS PharmSciTech. 2007 Oct 19;8(4):E86. (PMID: 18181547) Expert Opin Drug Deliv. 2016 Sep;13(9):1301-9. (PMID: 27110648) Oncol Rep. 2016 Oct;36(4):1875-85. (PMID: 27509880) Mol Pharm. 2009 Jul-Aug;6(4):1041-51. (PMID: 19366234) Pharm Res. 2006 Jul;23(7):1574-85. (PMID: 16786442) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) World J Gastroenterol. 2003 Dec;9(12):2642-9. (PMID: 14669304) Biomaterials. 2009 Mar;30(8):1627-34. (PMID: 19111339) Lancet Oncol. 2014 Dec;15(13):1481-1492. (PMID: 25456367) Int J Nanomedicine. 2019 Mar 19;14:1937-1952. (PMID: 30936695) Toxicol Mech Methods. 2021 Feb;31(2):138-149. (PMID: 33190582) Trends Pharmacol Sci. 2017 Feb;38(2):127-142. (PMID: 27916280) Cancer Lett. 2010 Dec 18;299(1):63-71. (PMID: 20826046) Int J Nanomedicine. 2012;7:95-107. (PMID: 22275826) J Biomed Biotechnol. 2011;2011:473964. (PMID: 21253581) J Natl Cancer Inst. 2001 Oct 17;93(20):1534-40. (PMID: 11604476) Molecules. 2020 Sep 23;25(19):. (PMID: 32977707) Expert Opin Drug Deliv. 2019 May;16(5):507-524. (PMID: 30957577) Adv Drug Deliv Rev. 2014 Feb;66:2-25. (PMID: 24270007) Drug Discov Today. 2015 Jan;20(1):95-104. (PMID: 25277320) BMC Cancer. 2020 Apr 28;20(1):354. (PMID: 32345258) Gastroenterology. 2003 Mar;124(3):762-77. (PMID: 12612914) |
فهرسة مساهمة: | Keywords: 5-flurouracil; PLGA-lecithin; apoptosis; experimental colon dysplasia; mouse; polymeric lipid hybrid nanoparticles |
المشرفين على المادة: | 0 (Ki-67 Antigen) 0 (Lecithins) 0 (Lipids) 0 (Polymers) 0 (RNA, Messenger) 0 (Tumor Suppressor Protein p53) 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer) U3P01618RT (Fluorouracil) |
تواريخ الأحداث: | Date Created: 20210120 Date Completed: 20210623 Latest Revision: 20210623 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC7830948 |
DOI: | 10.3390/biom11010109 |
PMID: | 33467560 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2218-273X |
---|---|
DOI: | 10.3390/biom11010109 |