دورية أكاديمية
Simultaneous Discovery of Positive and Negative Interactions Among Rhizosphere Bacteria Using Microwell Recovery Arrays.
| العنوان: | Simultaneous Discovery of Positive and Negative Interactions Among Rhizosphere Bacteria Using Microwell Recovery Arrays. |
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| المؤلفون: | Barua N; Tim Taylor Department of Chemical Engineering, Kansas State University, Manhattan, KS, United States., Herken AM; Division of Biology, Kansas State University, Manhattan, KS, United States., Stern KR; Tim Taylor Department of Chemical Engineering, Kansas State University, Manhattan, KS, United States., Reese S; Powers and Zahr, Augusta, KS, United States., Powers RL; Powers and Zahr, Augusta, KS, United States., Morrell-Falvey JL; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States., Platt TG; Division of Biology, Kansas State University, Manhattan, KS, United States., Hansen RR; Tim Taylor Department of Chemical Engineering, Kansas State University, Manhattan, KS, United States. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2021 Jan 05; Vol. 11, pp. 601788. Date of Electronic Publication: 2021 Jan 05 (Print Publication: 2020). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Understanding microbe-microbe interactions is critical to predict microbiome function and to construct communities for desired outcomes. Investigation of these interactions poses a significant challenge due to the lack of suitable experimental tools available. Here we present the microwell recovery array (MRA), a new technology platform that screens interactions across a microbiome to uncover higher-order strain combinations that inhibit or promote the function of a focal species. One experimental trial generates 10 4 microbial communities that contain the focal species and a distinct random sample of uncharacterized cells from plant rhizosphere. Cells are sequentially recovered from individual wells that display highest or lowest levels of focal species growth using a high-resolution photopolymer extraction system. Interacting species are then identified and putative interactions are validated. Using this approach, we screen the poplar rhizosphere for strains affecting the growth of Pantoea sp. YR343, a plant growth promoting bacteria isolated from Populus deltoides rhizosphere. In one screen, we montiored 3,600 microwells within the array to uncover multiple antagonistic Stenotrophomonas strains and a set of Enterobacter strains that promoted YR343 growth. The later demonstrates the unique ability of the platform to discover multi-membered consortia that generate emergent outcomes, thereby expanding the range of phenotypes that can be characterized from microbiomes. This knowledge will aid in the development of consortia for Populus production, while the platform offers a new approach for screening and discovery of microbial interactions, applicable to any microbiome. Competing Interests: RH and TP have filed a patent application on this technology. RP and SR were employed by the company Powers & Zhar. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Barua, Herken, Stern, Reese, Powers, Morrell-Falvey, Platt and Hansen.) |
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| فهرسة مساهمة: | Keywords: consortia; high throughput screening; microbial communities; microbial interactions; microbiome; microdevice; microwell; plant growth promoting rhizobacteria |
| تواريخ الأحداث: | Date Created: 20210120 Latest Revision: 20240330 |
| رمز التحديث: | 20240330 |
| مُعرف محوري في PubMed: | PMC7813777 |
| DOI: | 10.3389/fmicb.2020.601788 |
| PMID: | 33469450 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2020.601788 |