دورية أكاديمية
GPVI (Glycoprotein VI) Interaction With Fibrinogen Is Mediated by Avidity and the Fibrinogen αC-Region.
العنوان: | GPVI (Glycoprotein VI) Interaction With Fibrinogen Is Mediated by Avidity and the Fibrinogen αC-Region. |
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المؤلفون: | Xu RG; Discovery and Translational Science Department, Institute of Cardiovascular and Metabolic Medicine (R.-G.X., J.S.G., S.R.B., H.R.M., C.D., R.A.S.A.)., Gauer JS; Discovery and Translational Science Department, Institute of Cardiovascular and Metabolic Medicine (R.-G.X., J.S.G., S.R.B., H.R.M., C.D., R.A.S.A.)., Baker SR; Discovery and Translational Science Department, Institute of Cardiovascular and Metabolic Medicine (R.-G.X., J.S.G., S.R.B., H.R.M., C.D., R.A.S.A.).; Department of Physics, Wake Forest University, Winston Salem, NC (S.R.B.)., Slater A; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, United Kingdom (A.S., E.M.M., S.P.W.)., Martin EM; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, United Kingdom (A.S., E.M.M., S.P.W.)., McPherson HR; Discovery and Translational Science Department, Institute of Cardiovascular and Metabolic Medicine (R.-G.X., J.S.G., S.R.B., H.R.M., C.D., R.A.S.A.)., Duval C; Discovery and Translational Science Department, Institute of Cardiovascular and Metabolic Medicine (R.-G.X., J.S.G., S.R.B., H.R.M., C.D., R.A.S.A.)., Manfield IW; School of Molecular and Cellular Biology, Faculty of Biological Sciences (I.W.M.), University of Leeds, United Kingdom., Bonna AM; Department of Biochemistry, University of Cambridge, United Kingdom (A.M.B.)., Watson SP; Institute of Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, United Kingdom (A.S., E.M.M., S.P.W.)., Ariëns RAS; Discovery and Translational Science Department, Institute of Cardiovascular and Metabolic Medicine (R.-G.X., J.S.G., S.R.B., H.R.M., C.D., R.A.S.A.). |
المصدر: | Arteriosclerosis, thrombosis, and vascular biology [Arterioscler Thromb Vasc Biol] 2021 Mar; Vol. 41 (3), pp. 1092-1104. Date of Electronic Publication: 2021 Jan 21. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 9505803 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1524-4636 (Electronic) Linking ISSN: 10795642 NLM ISO Abbreviation: Arterioscler Thromb Vasc Biol Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 1998- : Baltimore, Md. : Lippincott Williams & Wilkins Original Publication: Dallas, TX : American Heart Association, c1995- |
مواضيع طبية MeSH: | Fibrinogen/*metabolism , Peptide Fragments/*blood , Platelet Membrane Glycoproteins/*metabolism, Animals ; Carrier Proteins/chemistry ; Carrier Proteins/metabolism ; Fibrin Fibrinogen Degradation Products/chemistry ; Fibrin Fibrinogen Degradation Products/metabolism ; Fibrinogen/chemistry ; Humans ; In Vitro Techniques ; Mice ; Microscopy, Atomic Force ; Peptide Fragments/chemistry ; Peptides/chemistry ; Peptides/metabolism ; Platelet Aggregation/physiology ; Platelet Membrane Glycoproteins/chemistry ; Protein Interaction Domains and Motifs ; Protein Structure, Quaternary ; Signal Transduction ; Surface Plasmon Resonance |
مستخلص: | Objective: GPVI (glycoprotein VI) is a key molecular player in collagen-induced platelet signaling and aggregation. Recent evidence indicates that it also plays important role in platelet aggregation and thrombus growth through interaction with fibrin(ogen). However, there are discrepancies in the literature regarding whether the monomeric or dimeric form of GPVI binds to fibrinogen at high affinity. The mechanisms of interaction are also not clear, including which region of fibrinogen is responsible for GPVI binding. We aimed to gain further understanding of the mechanisms of interaction at molecular level and to identify the regions on fibrinogen important for GPVI binding. Approach and Results: Using multiple surface- and solution-based protein-protein interaction methods, we observe that dimeric GPVI binds to fibrinogen with much higher affinity and has a slower dissociation rate constant than the monomer due to avidity effects. Moreover, our data show that the highest affinity interaction of GPVI is with the αC-region of fibrinogen. We further show that GPVI interacts with immobilized fibrinogen and fibrin variants at a similar level, including a nonpolymerizing fibrin variant, suggesting that GPVI binding is independent of fibrin polymerization. Conclusions: Based on the above findings, we conclude that the higher affinity of dimeric GPVI over the monomer for fibrinogen interaction is achieved by avidity. The αC-region of fibrinogen appears essential for GPVI binding. We propose that fibrin polymerization into fibers during coagulation will cluster GPVI through its αC-region, leading to downstream signaling, further activation of platelets, and potentially stimulating clot growth. Graphic Abstract: A graphic abstract is available for this article. |
References: | J Thromb Haemost. 2013 Sep;11(9):1751-9. (PMID: 23815599) Anal Biochem. 1993 Aug 1;212(2):457-68. (PMID: 8214588) J Biol Chem. 2002 Nov 29;277(48):46197-204. (PMID: 12356768) J Thromb Haemost. 2005 Aug;3(8):1752-62. (PMID: 16102042) EMBO J. 2001 May 1;20(9):2120-30. (PMID: 11331578) Nanoscale. 2017 Sep 21;9(36):13707-13716. (PMID: 28884176) J Biol Chem. 2006 Apr 14;281(15):10439-47. (PMID: 16455647) J Biol Chem. 1975 Sep 25;250(18):7210-8. (PMID: 126232) J Thromb Haemost. 2018 Feb;16(2):389-404. (PMID: 29210180) Blood. 2011 Mar 24;117(12):3460-8. (PMID: 21224475) J Exp Med. 2001 Feb 19;193(4):459-69. (PMID: 11181698) FEBS Lett. 1998 Jul 31;432(1-2):45-9. (PMID: 9710248) J Biol Chem. 2012 Aug 24;287(35):30000-13. (PMID: 22773837) J Biol Chem. 1999 Oct 8;274(41):29019-24. (PMID: 10506151) Biochemistry. 1975 Mar 11;14(5):940-6. (PMID: 123758) Thromb Haemost. 2020 Jun;120(6):977-993. (PMID: 32492725) Biochemistry. 2001 Jan 23;40(3):801-8. (PMID: 11170397) J Nat Med. 2018 Jan;72(1):32-42. (PMID: 29164507) Platelets. 2019;30(3):281-289. (PMID: 30110193) Eur J Immunol. 2000 Sep;30(9):2692-7. (PMID: 11009104) J Thromb Haemost. 2012 Dec;10(12):2418-27. (PMID: 23020554) Thromb Haemost. 2011 May;105 Suppl 1:S3-12. (PMID: 21479341) Chem Biol. 2009 Sep 25;16(9):990-1000. (PMID: 19778727) Blood Adv. 2017 Aug 15;1(19):1495-1504. (PMID: 29296791) Biochemistry. 1992 Feb 18;31(6):1579-84. (PMID: 1737014) J Thromb Haemost. 2011 Jul;9(7):1423-6. (PMID: 21535392) J Clin Invest. 2018 Aug 1;128(8):3356-3368. (PMID: 29723163) Blood. 2006 Aug 1;108(3):936-42. (PMID: 16861347) Bioinformatics. 2019 Dec 15;35(24):5326-5327. (PMID: 31263867) Biochemistry. 2002 Jun 25;41(25):7907-13. (PMID: 12069579) J Thromb Haemost. 2009 Aug;7(8):1356-63. (PMID: 19552682) Br J Pharmacol. 2013 Apr;168(8):1771-85. (PMID: 23330947) J Cell Biol. 2008 Aug 25;182(4):791-800. (PMID: 18710925) J Thromb Haemost. 2009 Aug;7(8):1344-6. (PMID: 19496922) Eur J Pharm Biopharm. 2019 Jan;134:37-48. (PMID: 30408518) J Clin Invest. 2013 Feb;123(2):908-16. (PMID: 23348738) J Biol Chem. 1993 Jun 25;268(18):13577-85. (PMID: 8514790) J Biol Chem. 2007 Oct 19;282(42):30434-41. (PMID: 17690106) Blood. 2003 Jul 15;102(2):449-61. (PMID: 12649139) Protein Sci. 2007 Mar;16(3):355-61. (PMID: 17322526) Biochemistry. 1996 May 7;35(18):5810-6. (PMID: 8639541) Arterioscler Thromb Vasc Biol. 2012 Mar;32(3):778-85. (PMID: 22155453) Methods Mol Biol. 2017;1654:151-164. (PMID: 28986788) Blood. 2015 Jul 30;126(5):683-91. (PMID: 25977585) Blood. 2015 Sep 24;126(13):1601-8. (PMID: 26282541) J Thromb Haemost. 2009 Jan;7(1):152-61. (PMID: 18983512) Protein Eng Des Sel. 2013 Oct;26(10):635-44. (PMID: 23924760) J Thromb Haemost. 2020 Apr;18(4):802-814. (PMID: 31889430) J Biol Chem. 2003 Sep 26;278(39):37154-9. (PMID: 12853452) J Thromb Haemost. 2017 Mar;15(3):549-564. (PMID: 28058806) Ann N Y Acad Sci. 2001;936:312-27. (PMID: 11460487) Biochemistry. 2007 Aug 7;46(31):9133-42. (PMID: 17630702) Haematologica. 2018 May;103(5):898-907. (PMID: 29472360) Biochemistry. 2002 May 21;41(20):6449-59. (PMID: 12009908) J Thromb Haemost. 2009 Aug;7(8):1347-55. (PMID: 19486274) Biochemistry. 2010 Sep 7;49(35):7643-51. (PMID: 20687529) |
معلومات مُعتمدة: | 204951 United Kingdom WT_ Wellcome Trust; 204951/B/16/Z United Kingdom WT_ Wellcome Trust; 062164/Z00/Z United Kingdom WT_ Wellcome Trust; 204951/Z/16/Z United Kingdom WT_ Wellcome Trust; CH/03/003/15571 United Kingdom BHF_ British Heart Foundation |
فهرسة مساهمة: | Keywords: fibrin; fibrinogen; glycoprotein VI; platelet aggregation; thrombosis |
المشرفين على المادة: | 0 (Carrier Proteins) 0 (Fibrin Fibrinogen Degradation Products) 0 (Peptide Fragments) 0 (Peptides) 0 (Platelet Membrane Glycoproteins) 0 (collagen-related peptide) 0 (fibrin fragment D) 0 (fibrinogen alphaC) 0 (platelet membrane glycoprotein VI) 9001-32-5 (Fibrinogen) |
تواريخ الأحداث: | Date Created: 20210121 Date Completed: 20210315 Latest Revision: 20220302 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC7901536 |
DOI: | 10.1161/ATVBAHA.120.315030 |
PMID: | 33472402 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1524-4636 |
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DOI: | 10.1161/ATVBAHA.120.315030 |