دورية أكاديمية
Influences of the 3D microenvironment on cancer cell behaviour and treatment responsiveness: A recent update on lung, breast and prostate cancer models.
| العنوان: | Influences of the 3D microenvironment on cancer cell behaviour and treatment responsiveness: A recent update on lung, breast and prostate cancer models. |
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| المؤلفون: | Costard LS; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland., Hosn RR; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland; Trinity Centre of Bioengineering (TCBE), Trinity College Dublin (TCD), Dublin, Ireland; Dept. of Mechanical and Manufacturing Engineering, School of Engineering, Trinity College Dublin (TCD), the University of Dublin, Ireland., Ramanayake H; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland., O'Brien FJ; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland; Trinity Centre of Bioengineering (TCBE), Trinity College Dublin (TCD), Dublin, Ireland; Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland., Curtin CM; Tissue Engineering Research Group (TERG), Department of Anatomy and Regenerative Medicine, Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland; Trinity Centre of Bioengineering (TCBE), Trinity College Dublin (TCD), Dublin, Ireland; Advanced Materials and Bioengineering Research Centre (AMBER), RCSI and TCD, Dublin, Ireland. Electronic address: carolinecurtin@rcsi.ie. |
| المصدر: | Acta biomaterialia [Acta Biomater] 2021 Sep 15; Vol. 132, pp. 360-378. Date of Electronic Publication: 2021 Jan 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101233144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-7568 (Electronic) Linking ISSN: 17427061 NLM ISO Abbreviation: Acta Biomater Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Kidlington, Oxford, UK : Elsevier, c2004- |
| مواضيع طبية MeSH: | Prostatic Neoplasms* , Tumor Microenvironment*, Animals ; Breast ; Cell Line, Tumor ; Humans ; Lung ; Male |
| مستخلص: | The majority of in vitro studies assessing cancer treatments are performed in two-dimensional (2D) monolayers and are subsequently validated in in vivo animal models. However, 2D models fail to accurately model the tumour microenvironment. Furthermore, animal models are not directly applicable to mimic the human scenario. Three-dimensional (3D) culture models may help to address the discrepancies of 2D and animal models. When cancer cells escape the primary tumour, they can invade at distant organs building secondary tumours, called metastasis. The development of metastasis leads to a dramatic decrease in the life expectancy of patients. Therefore, 3D systems to model the microenvironment of metastasis have also been developed. Several studies have demonstrated changes in cell behaviour and gene expression when cells are cultured in 3D compared to 2D and concluded a better comparability to cells in vivo. Of special importance is the effect seen in response to anti-cancer treatments as models are built primarily to serve as drug-testing platforms. This review highlights these changes between cancer cells grown in 2D and 3D models for some of the most common cancers including lung, breast and prostate tumours. In addition to models aiming to mimic the primary tumour site, the effects of 3D cell culturing in bone metastasis models are also described. STATEMENT OF SIGNIFICANCE: Most in vitro studies in cancer research are performed in 2D and are subsequently validated in in vivo animal models. However, both models possess numerous limitations: 2D models fail to accurately model the tumour microenvironment while animal models are expensive, time-consuming and can differ considerably from humans. It is accepted that the cancer microenvironment plays a critical role in the disease, thus, 3D models have been proposed as a potential solution to address the discrepancies of 2D and animal models. This review highlights changes in cell behaviour, including proliferation, gene expression and chemosensitivity, between cancer cells grown in 2D and 3D models for some of the most common cancers including lung, breast and prostate cancer as well as bone metastasis. Competing Interests: Declaration of Competing Interest The authors declare no competing financial interests. (Copyright © 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 3D cancer models; Bone metastasis; Breast cancer; Extracellular matrix; Lung cancer; Prostate cancer |
| تواريخ الأحداث: | Date Created: 20210123 Date Completed: 20210920 Latest Revision: 20210920 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.actbio.2021.01.023 |
| PMID: | 33484910 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-7568 |
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| DOI: | 10.1016/j.actbio.2021.01.023 |